返回 
加入收藏
左金果胶胶囊的处方优化及其对胃溃疡模型大鼠的保护作用
x
请在关注微信后，向客服人员索取文件
| 篇名： | 左金果胶胶囊的处方优化及其对胃溃疡模型大鼠的保护作用 |
| TITLE： | Formulation Optimization of Zuojin Pectin Capsule and Its Protective Effect on Gastric Ulcer Model Rats |
| 摘要： | 目的：优化左金果胶胶囊的处方，制备对胃溃疡具有保护作用的现代左金果胶胶囊剂。方法：以果胶、可溶性淀粉、糊精含量为考察因素，以胶囊剂中颗粒的成型性、吸湿性、流动性为考察指标，采用正交实验优化左金果胶胶囊的处方。以左金提取物浸膏粉末为原料，采用湿法制粒填充法制备左金果胶胶囊。采用高效液相色谱法测定左金果胶胶囊中盐酸巴马汀、盐酸小檗碱、吴茱萸碱、吴茱萸次碱的含量，并采用转篮法考察该胶囊剂在0.1mol/L盐酸溶液中的体外释药行为。通过灌胃75％乙醇复制胃溃疡大鼠模型，以胃溃疡指数、胃溃疡抑制率和胃组织病理切片结果为指标，初步考察左金果胶胶囊（剂量分别为54、108、216mg/kg）的胃溃疡保护作用。结果：左金果胶胶囊的最优处方为果胶含量45％、可溶性淀粉含量12％、糊精含量27％、木糖醇含量1％。体外释放结果表明，1h时左金果胶胶囊中盐酸巴马汀和盐酸小檗碱分别释放了53.76％、54.82％，二者均在8h左右释放完全，且均符合零级释放行为。不同剂量左金果胶胶囊均能不同程度地改善胃溃疡模型大鼠胃组织的溃疡损伤，显著降低其胃溃疡指数（P＜0.01），显著升高其胃溃疡抑制率和碘酸雪夫氏染色阳性表达面积百分比（P＜0.01）。结论：成功制得对胃溃疡具有保护作用并具有一定缓释效应的左金果胶胶囊。 |
| ABSTRACT： | OBJECTIVE：To optimize the form ulation of Zuojin pectin c apsules，and to prepare modern Zuojin pectin capsules with protective effects against gastric ulcers. METHODS ：The formulation of Zuojin pectin capsules was optimized with orthogonal test with the contents of pectin ，soluble starch and dextrin as factors ，using formability ，moisture absorption and flow ability as indicators. Zuojin pectin capsule was prepared by wet granulation filling method with Zuojin extract powder as raw material. The contents of palmatine hydrochloride ，berberine hydrochloride ，evodiamine and rutaecarpin were evaluated by HPLC. Basket method was used to investigate the release behavior of the capsule in 0.1 mol/L HCl solution. The gastric ulcer model of rats was established by intragastric administration of 75％ ethanol. Gastric ulcer index ，the inhibition rate of gastric ulcer and the pathological sections were used as indexes to investigate the protective effect of Zuojin pectin capsules （the doses were 54，108， 216 mg/kg）on gastric ulcer. RESULTS ：The optimal formulation of Zuojin pectin capsules included 45％ pectin，12％ soluble starch，27％ dextrin and 1％ xylitol. Results of in vitro drug ， release showed that palmatine hydrochloride and berberine， hydrochloride in Zuojin pectin capsules released 53.76％ and No.54.82％ respectively within 1 h，completely released at about 8 h， and conformed to the zero-order release behavior. 2492109374@qq.com Different doses of Zuojin pectin capsule could improve the ulcer injury of gastric tissue in gastric ulcer model rats to different extent ，and significantly reduced the gastric ulcer index（P＜0.01），significantly increased the inhibition rate of gastric ulcer and the percentage of positive expression area of Schiff ’s iodate staining （P＜0.01）. CONCLUSIONS ：Zuojin pectin capsule with protective effect on gastric ulcer and certain sustained- release effect is successfully prepared. |
| 期刊： | 2021年第32卷第19期 |
| 作者： | 杨贵前，刘文，陶玲，沈祥春，宋朔尧，张环，李和蓉，王守莉，石惠云 |
| AUTHORS： | YANG Guiqian，LIU Wen， TAO Ling，SHEN Xiangchun，SONG Shuoyao，ZHANG Huan，LI Herong，WANG Shouli，SHI Huiyun |
| 关键字： | 左金果胶胶囊；胃溃疡；正交实验；缓释效应；大鼠 |
| KEYWORDS： | Zuojin pectin capsule ；Gastric ulcer ；Orthogonal test ；Sustained-release effect ；Rat |
| 阅读数： | 218 次 |
| 本月下载数： | 4 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
