蒙药那如-3味的醇提工艺优化
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篇名: 蒙药那如-3味的醇提工艺优化
TITLE: Optimization of Ethanol Extraction Technology of Mongolian Medicine Naru- 3
摘要: 目的:优化蒙药那如-3味的醇提工艺。方法:以苯甲酰乌头原碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱、乌头碱、新乌头碱、次乌头碱、胡椒碱和没食子酸含量的综合评分为考察指标,料液比、乙醇体积分数、提取时间为考察因素,采用L(934)正交实验设计优化蒙药那如-3味的醇提工艺。结果:最优醇提工艺为料液比1∶10(g/mL)、乙醇体积分数75%、提取时间1.5h。经3次实验验证,那如-3味醇提物中上述8种成分的平均含量分别为1.69、1.48、14.69、0.28、0.05、0.08、26.01、17.33mg/g(RSD为0~4.96%,n=3),平均综合评分为19.03分(RSD=1.42%,n=3)。结论:优化所得那如-3味醇提工艺稳定、可行。
ABSTRACT: OBJECTIVE:To optim ize ethanol extraction technology of Mongolian medicine Naru- 3. METHODS :The L 9(34) orthogonal design was used to optimize ethanol extraction technology of Mongolian medicine Naru- 3 with solid-liquid ratio ,ethanol volume fraction and extraction time as factors ,using comprehensive scores for the contents of benzoylaconitine ,benzoylneoaconitine, benzoylhypoaconitine,aconitine,neoaconitine,hypoaconitine,piperine and gallic acid as indexes. RESULTS :The optimal ethanol extraction technology was that solid-liquid ratio of 1∶10(g/mL),ethanol volume fraction of 75%,extracting for 1.5 h. After 3 times of validation tests ,average contents of above 8 components in ethanol extract from Naru- 3 were 1.69,1.48,14.69,0.28, 0.05,0.08,26.01,17.33 mg/g(RSDs were 0-4.96%,n=3),respectively. Average comprehensive score was 19.03(RSD=1.42%, n=3). CONCLUSIONS :The optimal ethanol extraction technology of Mongolian medicine Naru- 3 is stable and feasible.
期刊: 2021年第32卷第16期
作者: 李君,顾艳丽,祖文,刘洋,白图雅,胡玉霞,张梦迪
AUTHORS: LI Jun,GU Yanli,ZU Wen,LIU Yang,BAI Tuya,HU Yuxia,ZHANG Mengdi
关键字: 那如-3味;蒙药;醇提工艺;工艺优化;综合评分
KEYWORDS: Naru-3;Mongolian me dicine;Ethanol extracti on technology ;Technology optimization ;Comprehensive score
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