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和肤止痒方的UPLC指纹图谱建立及8种成分的含量测定
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| 篇名： | 和肤止痒方的UPLC指纹图谱建立及8种成分的含量测定 |
| TITLE： | Establishment of UPLC Fingerprint of Hefu Zhiyang Decoction and Content Determination of 8 Components |
| 摘要： | 目的：建立和肤止痒方的指纹图谱并进行聚类分析，建立该方中8种成分的含量测定方法，为其质量控制提供参考。方法：采用超高效液相色谱法（UPLC）。以ACQUITYUPLCBEHC18为色谱柱，以乙腈-0.1％冰醋酸溶液为流动相进行梯度洗脱，流速为0.25mL/min，柱温为30℃，检测波长为236nm，进样量为5μL。使用《中药色谱指纹图谱相似度评价系统（2012版）》建立10批和肤止痒方的指纹图谱，与对照品比对指认色谱峰，并进行相似度评价。使用SPSS21.0软件对10批样品进行聚类分析。采用上述UPLC法测定10批样品中没食子酸等8种成分的含量。结果：从10批和肤止痒方的UPLC指纹图谱中标定共有峰34个，指认出峰1为没食子酸、峰10为阿魏酸、峰13为落新妇苷、峰23为丹皮酚、峰28为白鲜碱、峰31为黄柏酮、峰32为梣酮、峰34为蛇床子素，与对照指纹图谱的相似度为0.923～0.979。10批样品中，S1、S2、S5、S6、S7、S8、S10聚为一类，S3、S4、S9聚为一类。上述8种成分的平均含量分别为0.596～0.714、0.262～0.321、7.647～9.859、0.113～0.644、0.170～0.202、0.854～1.281、0.631～0.857、3.243～3.548mg/g。结论：成功建立了和肤止痒方的UPLC指纹图谱和8种成分的含量测定方法。 |
| ABSTRACT： | OBJECTIVE：To establish th e fingerp rint and c onduct cluster analysis of Hefu zhiyang decoction （HFZYD），and establish the method for content determination of 8 components，so as to provide reference for the quality control of HFZYD. METHODS：UPLC method was adopted. The determination was performed on ACQUITY UPLC BEH C 18 column with mobile phase consisted of acetonitrile- 0.1％ glacial acetic acid solution （gradient elution ）at the flow rate of 0.25 mL/min. The column temperature maintained at 30 ℃，and detection wavelength was 236 nm. The sample size was 5 μL. The fingerprint of 10 batches of HFZYD was established with Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 edition）. Compared with substance control ，the common peaks were identified. The similarity evaluation was conducted. SPSS 21.0 software was used to conduct cluster analysis of 10 batches of samples. The contents of 8 components such as gallic acid in 10 batches of samples were determined by above UPLC. RESULTS ：There were 34 common peaks in the UPLC fingerprint of 10 batches of HFZYD ， identified as peak 1 was gallic acid ，peak 10 was ferulic acid ，peak 13 was astilbin ，peak 23 was paeonol ，peak 28 was dictamnine，peak 31 was obacunone ，peak 32 was fraxinellone ，and peak 34 was osthole. Its similarity with the control fingerprint was 0.923-0.979. Among 10 batches of samples ，S1，S2，S5，S6，S7，S8 and S 10 were clustered into one category ，and S 3，S4 and S 9 were clustered into one category. The average contents of above 8 components were 0.596-0.714，0.262-0.321，7.647-9.859， 0.113-0.644，0.170-0.202，0.854-1.281，0.631-0.857，3.243-3.548 mg/g，respectively. CONCLUSIONS ：UPLC fingerprint and the method for content determination of 8 components in HFZYD are established successfully. |
| 期刊： | 2021年第32卷第16期 |
| 作者： | 刘丽娟，赵亚，吴亚运，赵瑞芝，卢传坚 |
| AUTHORS： | LIU Lijuan ，ZHAO Ya，WU Yayun ，ZHAO Ruizhi ，LU Chuanjian |
| 关键字： | 和肤止痒方；超高效液相色谱法；指纹图谱；含量测定；聚类分析 |
| KEYWORDS： | Hefu zhiyang decoction ；UPLC；Fingerprint； |
| 阅读数： | 276 次 |
| 本月下载数： | 4 次 |
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