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桑根酮C对游离脂肪酸诱导人肝癌HepG2细胞脂质蓄积的改善作用
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| 篇名： | 桑根酮C对游离脂肪酸诱导人肝癌HepG2细胞脂质蓄积的改善作用 |
| TITLE： | Improvement Effects of Sanggenone C on Lipid Accumulation in FFA-induced Human Liver Cancer HepG 2 Cells |
| 摘要： | 目的：研究桑根酮C对游离脂肪酸诱导的人肝癌HepG2细胞脂质蓄积的改善作用。方法：将HepG2细胞分为对照组、模型组、非诺贝特组（10μmol/L）和桑根酮C低、中、高浓度组（2、4、8μmol/L），除对照组外，其余各组细胞加入1mmol/L游离脂肪酸以诱导建立脂质蓄积模型，且各给药组加入相应含药培养基进行培养。采用油红O染色法观察细胞中脂质蓄积情况，并测定脂质水平和三酰甘油（TG）含量；采用实时荧光定量聚合酶链式反应法和Westernblot法检测细胞中过氧化物酶体增殖物激活受体α（PPARα）、肉碱棕榈酰转移酶1（CPT-1）、成脂甾醇元件结合蛋白固醇调节元件结合蛋白1c（SREBP-1c）、脂肪酸合成酶（FAS）、沉默信息调节因子1（SIRT1）、过氧化物酶体增殖物激活受体γ辅助激活物1α（PGC-1α）的mRNA和蛋白表达水平。结果：与对照组比较，模型组细胞核明显萎缩、体积变小，脂滴数明显增加；细胞中脂质水平、TG含量以及SREBP-1c、FAS的mRNA和蛋白表达水平均显著升高（P＜0.05或P＜0.01），PPARα、CPT-1、SIRT1、PGC-1α的mRNA和蛋白表达水平均显著降低（P＜0.01）。与模型组比较，桑根酮C各浓度组细胞核萎缩不明显、体积大小正常，脂滴数明显减少；细胞中脂质水平、TG含量以及上述PPARα通路相关基因的mRNA和蛋白（桑根酮C低浓度组的SREBP-1c蛋白除外）表达水平均显著逆转（P＜0.05或P＜0.01）。结论：桑根酮C可改善HepG2细胞的脂质蓄积；其作用机制可能与调节PPARα信号通路、提高细胞脂质氧化能力、抑制脂质合成有关。 |
| ABSTRACT： | OBJECTIVE：To study the improvement ef fects of sanggenone C on lipid accumulation in human liver cancer HepG2 cells induced by free fatty acid （FFA）. METHODS ：HepG2 cells were divided into control group ，model group ， fenofibrate group （10 μmol/L），sangerone C low ，medium and high concentration groups （2，4，8 μmol/L）. Except for control group，other groups were treated with 1 mmol/L FFA to induce lipid accumulation model ，and administration groups were cultured with relevant medium containing drugs. The lipid accumulation was observed by oil red O staining ，and lipid level and triglyceride （TG） content were also determined. Real-time PCR and Western blot assay were adopted to detect the mRNA and protein expression of PPARα，CPT-1，SREBP-1c，FAS，SIRT1 and PGC- 1α in HepG2 cells. RESULTS ：Compared with control group ， the nucleus was atrophied significantly and the volume became smaller ，and the number of lipid droplets was significantly increased；the level of lipid ，TG content ，mRNA and protein expression of SREBP- 1c and FAS were significantly increased （P＜ 0.05 or P＜0.01），mRNA and protein expression of PPARα，CPT-1，SIRT1 and PGC- 1α were decreased significantly（P＜0.01）. Compared with model group ，no obvious nucleus atrophy and normal volume were observed in sangerone C groups ，and the number of lipid droplets was significantly reduced ；the levels of lipid ，TG content ，mRNA and protein expression of PPARα pathway related genes （except for SREBP- 1c protein in saggenone C low concentration group ）were significantly reversed （P＜ 0.05 or P＜0.01）. CONCLUSIONS ：Sangenone C can significantly improve the lipid accumulation of HepG 2 cells，and its mechanism may associated with regulating PPAR α signaling pathway，improving cell lipid oxidation ability and inhibiting lipid synthesis. |
| 期刊： | 2021年第32卷第15期 |
| 作者： | 邢菊玲，刘芬，冯萌，郝梦娇，黄天来，周欣欣，汤湘江 |
| AUTHORS： | XING Juling，LIU Fen，FENG Meng，HAO Mengjiao ，HUANG Tianlai ，ZHOU Xinxin ，TANG Xiangjiang |
| 关键字： | 桑根酮C；HepG2细胞；脂质蓄积；PPARα信号通路 |
| KEYWORDS： | Sanggenone C ；HepG2 cells；Lipid accumu- |
| 阅读数： | 323 次 |
| 本月下载数： | 4 次 |
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