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HPLC法同时测定不同基原崖桑皮和桑白皮中7种活性成分含量
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| 篇名： | HPLC法同时测定不同基原崖桑皮和桑白皮中7种活性成分含量 |
| TITLE： | Simultaneous Determination of 7 Active Components in Mori Australis Cortex and Mori Cortex from Different Sources by HPLC |
| 摘要： | 目的：建立同时测定重庆地区不同基原崖桑皮和桑白皮中7种活性成分含量的方法，为完善崖桑皮和桑白皮的质量控制标准以及比较两者质量等同性提供参考。方法：采用高效液相色谱（HPLC）法测定58批崖桑皮和桑白皮药材中新绿原酸、桑皮苷A、绿原酸、紫云英苷、山柰酚、桑辛素和异槲皮素的含量，色谱柱为DiamonsilC18，流动相为0.1％甲酸溶液-乙腈（梯度洗脱），流速为1.0mL/min，检测波长为280nm，柱温为30℃，进样量为10μL。利用SPSS22.0软件，采用独立样本t检验、主成分分析和聚类分析方法分析58批崖桑皮和桑白皮药材中上述7种活性成分的含量差异。结果：上述7种活性成分在各自质量浓度范围内与峰面积的线性关系良好（r≥0.9990），精密度、稳定性（24h）、重复性、耐用性和加样回收率试验的RSD均小于3％。崖桑皮中新绿原酸、桑皮苷A、绿原酸、紫云英苷、山柰酚、桑辛素和异槲皮素的平均含量分别为0.304、22.462、1.730、1.308、1.593、2.842、0.657mg/g，而桑白皮中上述7种活性成分的平均含量分别为0.305、22.995、2.486、2.438、2.916、4.158、1.264mg/g。独立样本t检验结果显示，崖桑皮和桑白皮的上述7种活性成分中只有山柰酚的含量差异具有统计学意义（P＜0.05）；主成分分析和聚类分析结果均显示崖桑皮中上述7种活性成分的含量与桑白皮无明显差别。结论：所建HPLC法简便、灵敏、准确度高，可为完善崖桑皮和桑白皮的质量控制标准提供参考。崖桑皮和桑白皮在主要活性成分上具有一定的质量等同性，以华桑和鸡桑为来源的崖桑皮可作为桑白皮的代替品使用。 |
| ABSTRACT： | OBJECTIVE：To establish a meth od for the simultaneous determination of 7 active components in Mori Australis Cortex and Mori Cortex from different sources in Chongqing area ，so as to provide reference for improving the quality control standards of Mori Australis Cortex and Mori Cortex and comparing the equivalence of their quality. METHODS ：HPLC method was used to determine the contents of neochlorogenic acid ，mulberroside A ，chlorogenic acid ，astragalin，kaempferol，morusin and isoquercetin in 58 batches of Mori Australis Cortex and Mori Cortex. The chromatographic column was Diamonsil C 18 with mobile phase consisted of 0.1％ formic acid solution-acetonitrile （gradient elution ） at the flow rate of 1.0 mL/min. The detection wavelength was 280 nm，column temperature was 30 ℃，and the injection volume was 10 μL. Using SPSS 22.0 software， independent sample t-test，principal component analysis and cluster analysis were used to analyze the content difference of the above-mentioned 7 active components in Mori Australis Cortex and Mori Cortex. RESULTS ：There was a good linear relationship between the peak area and the concentration of the above 7 active components （r≥0.999 0）. The RSDs of precision ，stability（24 h），repeatability，durability and recovery were less than 3％. The average contents of neochlorogenic acid ，mulberroside A ， chlorogenic acid ， astragalin， kaempferol， morusin and 023-58576130。E-mail：1025473978@qq.com isoquercetin in Mori Australis Cortex were 0.304，22.462， 1.730，1.308，1.593，2.842 and 0.657 mg/g，respectively. Those of Mori Cortex were 0.305，22.995，2.486，2.438， 2.916，4.158 and 1.264 mg/g，respectively. The results of independent sample t-test showed that only the content of kaempferol in the above 7 active components of Mori Australis Cortex and Mori Cortex had significant difference （P＜0.05）. The results of principal component analysis and cluster analysis showed that there was no significant difference in the contents of above 7 active components between Mori Australis Cortex and Mori Cortex. CONCLUSIONS：The established HPLC method is simple ，sensitive and accurate ，which can provide a reference for improving the quality control standard of Mori Australis Cortex and Mori Cortex. Mori Australis Cortex and Mori Cortex have certain quality equivalence in main active components ，and the Mori Australis Cortex from M. australis and M. cathayana can be used as a substitute for the Mori Cortex. |
| 期刊： | 2021年第32卷第15期 |
| 作者： | 黎海灵，黄艳萍，陈旭冰，谷文超，周游，张德全，周浓 |
| AUTHORS： | LI Hailing ，HUANG Yanping ，CHEN Xubing ，GU Wenchao ，ZHOU You，ZHANG Dequan ，ZHOU Nong |
| 关键字： | 崖桑皮；桑白皮；新绿原酸；桑皮苷A；绿原酸；紫云英苷；山柰酚；桑辛素；异槲皮素；含量；基原 |
| KEYWORDS： | Mori Australis Cortex ；Mori Cortex ；Neochlorogenic acid ；Mulberroside A ；Chlorogenic acid ；Astragalin； |
| 阅读数： | 331 次 |
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