岭南铁角蕨镇咳活性成分分析
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篇名: 岭南铁角蕨镇咳活性成分分析
TITLE: Analysis of Antitussive Active Ingredients of Asplenium sampsoni
摘要: 目的:研究岭南铁角蕨的化学成分并初步探讨其镇咳作用。方法:以硅胶柱色谱对岭南铁角蕨75%乙醇提取物进行分离纯化,根据理化性质和波谱(质谱、氢谱、碳谱)数据分析鉴定所得化合物的结构。将昆明种小鼠随机分为阴性对照组、测试组(分离所得7种单体化合物的剂量均为0.12g/kg)和阳性对照组(同剂量磷酸苯丙哌林),每组10只;采用浓氨水引咳法评价各化合物的镇咳效果。结果:从岭南铁角蕨75%乙醇提取物的乙酸乙酯部位和石油醚部位中共分离和鉴定出13个化合物,分别为4-羟基-苯乙酮(Ⅰ)、4-羟基-苯乙酸乙酯(Ⅱ)、木犀草素(Ⅲ)、芹菜素(Ⅳ)、槲皮素(Ⅴ)、芒柄花素(Ⅵ)、刺槐黄素(Ⅶ)、原儿茶酸(Ⅷ)、异迷迭香酸苷(Ⅸ)、槲皮素-3-O-β-D-吡喃葡萄糖苷(Ⅹ)、异鼠李素-3-O-β-D-吡喃葡萄糖苷(Ⅺ)、棕榈酸甲酯(Ⅻ)和欧前胡素(XⅢ)。其中木犀草素可显著延长小鼠的咳嗽潜伏期并减少小鼠的咳嗽次数(P<0.05)。结论:13种化合物均为首次从该植物中分离得到;木犀草素具有一定的镇咳作用,可能是该植物的镇咳活性成分。
ABSTRACT: OBJECTIVE:To study chemical constitue nts of Asplenium sampsoni ,and to investigate its antitussive effects preliminarily. METHODS : The 75% ethanol extract was isolated from A. sampsoni and purified by silica gel column chromatography. The structures of the compounds were identified by physicochemical properties and spectral data (mass spectrum , hydrogen spectrum and carbon spectrum ). Kunming mice were randomly divided into negative control group ,test group (the doses of 7 monomers were 0.12 g/kg),and positive control group (same dose of benproperine phosphate ),with 10 mice in each group. The antitussive effect of each compound was evaluated by the method of inducing cough with concentrated ammonia water. RESULTS:Totally 13 compounds were isolated and identified from ethyl acetate and petroleum ether parts of 75% ethanol extract of A. sampsoni ,i.e. 4-hydroxy-acetophenone(Ⅰ),4-hydroxy-ethyl phenylacetate (Ⅱ),luteolin(Ⅲ),apigenin(Ⅳ),quercetin (Ⅴ),formononetin(Ⅵ),acacetin(Ⅶ),protocatechuic acid (Ⅷ),isorosmarinoside(Ⅸ),quercetin-3-O-β-D-glucopyranoside (Ⅹ),isorhamnetin-3-O-β-D-glucopyranoside(Ⅺ),methyl palmitate (Ⅻ)and imperatorin (XⅢ). Luteolin could significantly prolong the cough latency and reduce the times of cough in mice (P<0.05). CONCLUSIONS :Thirteen compounds are isolated from this plant for the first time ;luteolin has certain antitussive effect ,and may be the antitussive active component of the plant.
期刊: 2021年第32卷第15期
作者: 李君,梁晓
AUTHORS: LI Jun ,LIANG Xiao
关键字: 岭南铁角蕨;化学成分;结构鉴定;镇咳作用
KEYWORDS: Asplenium sampsoni ;Chemical constituents ;Structure identification ;Antitussive effect
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