烟管头草水提物对前列腺癌PC3细胞增殖、迁移与侵袭的影响
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篇名: 烟管头草水提物对前列腺癌PC3细胞增殖、迁移与侵袭的影响
TITLE: Effects of Water Extract from Carpesium cernuum on Proliferation ,Metastasis and Invasion of Prostate Cancer PC3 Cells
摘要: 目的:研究烟管头草水提物(AECC)对前列腺癌PC3细胞增殖、迁移与侵袭的影响。方法:将细胞分为对照组和AECC不同质量浓度组(5、10、20、40、80μg/L),加入相应药物或培养基培养不同时间(24、48、72h)后,检测细胞存活率。将细胞分为对照组和AECC低、中、高质量浓度组(20、40、80μg/L),同法培养24h后,采用Hoechst33258染色法和流式细胞仪检测各组细胞的凋亡情况;采用Transwell实验检测细胞迁移数和侵袭数;采用实时荧光定量聚合酶链式反应和Westernblot法检测AECC低、中质量浓度组细胞中β-连环蛋白(β-catenin)信号通路相关迁移与凋亡蛋白[β-catenin、基质金属蛋白酶7(MMP-7)、髓细胞瘤病毒致癌基因(c-Myc)、胱天蛋白酶3(caspase-3)、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)]的mRNA和蛋白表达水平。结果:随着给药浓度的增加和作用时间的延长,AECC不同质量浓度组细胞存活率均显著低于对照组(P<0.05或P<0.01),且呈不断降低趋势。与对照组比较,AECC各质量浓度组细胞的早期凋亡率(中质量浓度组除外),细胞迁移数和侵袭数,以及AECC低、中质量浓度组细胞中MMP-7、c-Myc(低质量浓度组除外)、Bcl-2(低质量浓度组mRNA除外)的mRNA和蛋白表达水平均显著降低(P<0.05或P<0.01);AECC各质量浓度组细胞的晚期凋亡率,AECC低、中质量浓度组细胞中β-catenin、caspases-3(低质量浓度组除外)、Bax(低质量浓度组mRNA除外)的mRNA和蛋白表达水平均显著升高(P<0.05或P<0.01)。结论:AECC可抑制PC3细胞的增殖、迁移与侵袭,其作用机制可能与调控β-catenin信号通路相关的迁移与凋亡因子的表达有关。
ABSTRACT: OBJECTIVE:To study the effe cts of the water extract from Carpesium cernuum (AECC)on the proliferation , metastasis and invasion of prostate cancer PC 3 cells. METHODS :Cells were divided into control group and different concentration groups of AECC (5,10,20,40,80 μg/L),and then treated with relevant medicine or medium for different time (24,48,72 h). The survival rates of cells were detected. Cells were divided into control group ,and AECC low ,medium and high concentration groups(20,40,80 μg/L). After cultured for 24 h,Hoechst 33258 staining and flow cytometry were used to detect the apoptosis of cells. The number of cell metastasis and invasion were detected by Transwell assay. RT-qPCR and Western blot assay were applied to detect the mRNA and protein expression of β-catenin signaling pathway related migration and apoptosis proteins (β-catenin, MMP-7,c-Myc,caspase-3,Bcl-2 and Bax )in AECC low and medium concentration groups. RESULTS :With the increase of the concentration and culture time ,the survival rates of cells in AECC different concentration groups were significantly lower than control group (P<0.05 or P<0.01),and showed a decreasing trend. Compared with control group ,the early apoptosis rate (except the medium concentration group )and the number of cell metastasis and invasion in AECC groups ,the mRNA and protein expression of MMP- 7,c-Myc(except for the low concentration group )and Bcl- 2(except for mRNA of the low concentration group)in AECC low and medium concentration groups were decreased significantly (P<0.05 or P<0.01). Late apoptosis rate of AECC groups ,the mRNA and protein expression of β-catenin,caspases-3(except for the low concentration group ),Bax(except for mRNA of the low concentration group )in AECC low and medium concentration groups were increased significantly (P<0.05 or P<0.01). CONCLUSIONS :AECC could inhibit the proliferation ,metastasis and invasion of PC 3 cells;the mechanism of which may be associated with regulating the expression of β-catenin signaling pathway related migration and apoptotic factors.
期刊: 2021年第32卷第15期
作者: 刘波,余佳,王坤,晏晨,黄丽荣,骆衡
AUTHORS: LIU Bo,YU Jia,WANG Kun,YAN Chen,HUANG Lirong ,LUO Heng
关键字: 烟管头草;前列腺癌PC3细胞;β-连环蛋白;增殖;迁移;侵袭
KEYWORDS: Carpesium cernuum ;Prostate cancer PC 3 cells;β-catenin;Proliferation;Metastasis;Invasion
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