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半夏野生品和栽培品中8种核苷类成分的含量测定方法建立及差异分析
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| 篇名： | 半夏野生品和栽培品中8种核苷类成分的含量测定方法建立及差异分析 |
| TITLE： | Method Establishment for Content Determination of 8 Nucleosides in Wild and Cultivated Pinellia ternata and the Difference Analysis |
| 摘要： | 目的：建立半夏野生品和栽培品中8种核苷类成分含量测定的方法，并进行两者的差异分析。方法：采用高效液相色谱法测定20批半夏药材（野生品YS1～YS8、栽培品ZP1～ZP12）中尿嘧啶、尿苷、肌苷、黄嘌呤、腺嘌呤、鸟苷、β-胸苷和腺苷的含量；以上述8种核苷类成分含量为基础，采用聚类分析、主成分分析（PCA）、偏最小二乘法-判别分析（OPLS-DA）等化学模式识别法对半夏野生品和栽培品进行综合评价。结果：20批半夏药材中尿嘧啶、黄嘌呤、尿苷、肌苷、腺嘌呤、鸟苷、β-胸苷和腺苷的含量分别为0.02～0.24、0.01～0.24、0.06～0.37、0.02～0.14、0.04～0.22、0.14～0.42、0.01～0.09、0～0.32mg/g。经聚类分析、PCA和OPLS-DA发现，8批野生品（YS1～YS8）聚为一类，12批栽培品（ZP1～ZP12）聚为一类；半夏野生品的主要特征标志物为鸟苷、尿苷、腺苷、腺嘌呤，半夏栽培品的主要特征标志物为尿嘧啶、黄嘌呤、肌苷、β-胸苷。结论：建立了半夏药材中8种核苷类成分的含量测定方法；以核苷类成分为质量标志物可有效区分半夏野生品和栽培品，且野生品质量优于栽培品。 |
| ABSTRACT： | OBJECTIVE：To establish the metho d for the content determination of 8 nucleosides in wild and cultivated Pinellia ternate，and to conduct the difference analysis. METHODS ：The contents of uracil ，uridine，inosine，xanthine，adenine， guanosine，β-thymidine and adenosine in 20 batches of P. ternate （wild product YS 1-YS8，cultivated product ZP 1-ZP12）were determined by HPLC method. Based on the contents of the above 8 nucleosides，cluster analysis ，principal component analysis （PCA），partial least squares analysis （OPLS-DA）were used to comprehensively evaluate the wild and cultivated P. ternata . RESULTS：The contents of uracil ，uridine，inosine，xanthine，adenine，guanosine，β-thymidine and adenosine in 20 batches of P. ternate were 0.02-0.24，0.01-0.24，0.06-0.37，0.02-0.14，0.04-0.22，0.14-0.42，0.01-0.09，0-0.32 mg/g，respectively. Cluster analysis，PCA and OPLS-DA showed that 8 batches of wild P. ternate （YS1-YS8）were clustered into one category ，and 12 batches of cultivated P. ternate （ZP1-ZP12）were clustered into one category. Main characteristic markers of wild P. ternate were guanosine，uridine，adenosine and adenine ，while the main characteristic markers of cultivated P. ternate were urinine ，xanthine， inosine，and β-thymidine. CONCLUSIONS：The method for the content determination of 8 nucleosides in P. ternate is established. Nucleosides as quality markers can effectively distinguish wild and cultivated P. ternata ，and the quality of the wild P. ternate was better than that of cultivated P. ternate . |
| 期刊： | 2021年第32卷第13期 |
| 作者： | 张勇，李传峰，高桂花，马瑜，任强 |
| AUTHORS： | ZHANG Yong，LI Chuanfeng，GAO Guihua，MA Yu，REN Qiang |
| 关键字： | 半夏；野生品；栽培品；核苷类；含量测定；化学模式识别法 |
| KEYWORDS： | Pinellia ternata ；Wild product ；Cultivated product ；Nucleoside；Content determination ；Chemical pattern |
| 阅读数： | 235 次 |
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