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β-乳香酸对海马神经元细胞氧糖剥夺损伤的改善作用
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| 篇名： | β-乳香酸对海马神经元细胞氧糖剥夺损伤的改善作用 |
| TITLE： | Improvement Effects of β-boswellic Acid on Hippocampal Neurons Cells Injury Induced by Oxygen-glucose Deprivation |
| 摘要： | 目的：研究β-乳香酸对大鼠海马神经元细胞氧糖剥夺损伤的改善作用。方法：将大鼠海马神经元细胞分为正常对照组、模型组和β-乳香酸低、中、高浓度组（1、10、100μmol/L），除正常对照组外，其余各组细胞加入相应含药培养基，并进行氧糖剥夺培养以复制氧糖剥夺损伤模型。采用MTT法检测细胞的存活率，采用化学比色法检测细胞上清液中乳糖脱氢酶（LDH）活性，采用Hoechst-PI染色法观察细胞形态的变化，采用流式细胞仪检测细胞早期凋亡率，采用Westernblot法检测细胞中凋亡相关蛋白[B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、激活型胱天蛋白酶3（cleavedcaspase-3）]的表达。结果：与模型组比较，β-乳香酸中、高浓度组细胞存活率、Bcl-2蛋白表达水平均显著升高（P＜0.01），细胞上清液中LDH活性、细胞早期凋亡率和细胞中cleavedcas-pase-3、Bax蛋白表达水平均显著降低（P＜0.05或P＜0.01）；细胞核致密浓染、碎片状现象明显减少。结论：β-乳香酸可改善大鼠海马神经元细胞氧糖剥夺损伤，其作用机制可能与下调cleavedcaspase-3、Bax蛋白表达，上调Bcl-2蛋白表达有关。 |
| ABSTRACT： | OBJECTIVE：To study the imp rovement effects of β-boswellic acid on hippocampal neurons cells injury of rats induced by oxygen-glucose deprivation. METHODS ：The hippocampal neurons cell of rats were divided into normal control group ， model group and β-boswellic acid low-concentration ，medium-concentration and high-concentration groups （1，10，100 μmol/L）. Except for normal control group ，other groups were cultured with relevant medium and given oxygen glucose deprivation to induce oxygen-glucose deprivation induced injury model. MTT assay was adopted to detect cell viability. Chemical colorimetry was used to detect LDH activity in cell culture supernatant. Hoechst-PI staining was used to detect the morphology change of cells. Flow cytometry was used to detect early apoptosis rate of cells. The expression of apoptosis-related protein （Bcl-2，Bax and cleaved caspase-3） were detected by Western blot. RESULTS ：Compared with model group ，the survival rate of cells and protein expression of Bcl- 2 were increased significantly in β-boswellic acid medium-concentration and high-concentration groups （P＜ 0.01），while LDH activity ，early apoptosis rate ，protein expression of cleaved caspase- 3 and Bax were all decreased significantly （P＜0.05 or P＜0.01）. The densely stained nuclei and fragmentation decreased significantly. CONCLUSIONS ：β-boswellic acid can relieve oxygen-glucose deprivation induced injury of hippocampal neurons cells ，the mechanism of which may be associated with down-regulating the protein expression of cleaved caspase- 3 and Bax and up-regulating the protein expression of Bcl- 2. |
| 期刊： | 2021年第32卷第11期 |
| 作者： | 王明明，王磊，窦芳，李韦韦，文爱东，王婧雯 |
| AUTHORS： | WANG Mingming，WANG Lei，DOU Fang，LI Weiwei，WEN Aidong，WANG Jingwen |
| 关键字： | β-乳香酸；海马神经元细胞；氧糖剥夺；细胞凋亡 |
| KEYWORDS： | β-boswellic acid ；Hippocampal neurons cells ；Oxygen-glucose deprivation ；Cell apoptosis |
| 阅读数： | 657 次 |
| 本月下载数： | 6 次 |
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