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黄芪甲苷对变应性鼻炎模型小鼠改善作用的机制初探
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| 篇名： | 黄芪甲苷对变应性鼻炎模型小鼠改善作用的机制初探 |
| TITLE： | Preliminary Study on the Improvement Effects of Astragaloside Ⅳ on Allergic Rhinitis Model Mice |
| 摘要： | 目的：初步研究黄芪甲苷对变应性鼻炎（AR）模型小鼠改善作用的可能机制。方法：将C57/BL6小鼠随机分为空白组、模型组和黄芪甲苷组，每组10只。除空白组外，其余大鼠均于第0、7、14、21～27天以卵清蛋白为致敏源进行攻击以复制AR模型。于造模第15～27天，黄芪甲苷组小鼠按0.02mL/g腹腔注射黄芪甲苷40mg/kg（第21～27天均于攻击致敏前1h给药），空白组和模型组小鼠腹腔注射等体积生理盐水，每天1次。末次攻击致敏24h后，观察各组小鼠鼻黏膜组织中炎症细胞的浸润情况，检测其鼻腔灌洗液中白细胞介素4（IL-4）、IL-5、干扰素γ（IFN-γ）含量，鼻黏膜和脾脏组织中活性氧簇（ROS）水平和磷酸化Janus激酶2（p-JAK2）、磷酸化信号转导及转录激活蛋白6（p-STAT6）阳性细胞计数，以及脾脏组织中JAK2、STAT6蛋白的磷酸化水平（即p-JAK2/JAK2比值、p-STAT6/STAT6比值）。结果：与空白组比较，模型组小鼠鼻黏膜组织中炎症细胞（嗜酸粒细胞和肥大细胞）计数，鼻腔灌洗液中IL-4、IL-5含量，鼻黏膜和脾脏组织中ROS水平和p-JAK2、p-STAT6阳性细胞计数，脾脏组织中p-JAK2/JAK2比值、p-STAT6/STAT6比值均显著升高（P＜0.05），鼻腔灌洗液中INF-γ含量显著降低（P＜0.05）。与模型组比较，黄芪甲苷组小鼠鼻黏膜组织中炎症细胞计数，鼻腔灌洗液中IL-4、IL-5含量，鼻黏膜和脾脏组织中ROS水平和p-JAK2、p-STAT6阳性细胞数量，脾脏组织中p-JAK2/JAK2比值、p-STAT6/STAT6比值均显著降低（P＜0.05），鼻腔灌洗液中INF-γ含量显著增加（P＜0.05）。结论：黄芪甲苷能够有效改善AR模型小鼠变应性炎症反应，其机制可能与下调JAK2/STAT6信号通路及ROS水平有关。 |
| ABSTRACT： | OBJECTIVE：To preliminarily s tudy the potential mechanism of astragaloside Ⅳ on allergic rhinitis （AR）model mice. METHODS ：C57/BL6 mice were randomly divided into blank group ，model group and astragaloside Ⅳ group，with 10 mice in each group. Except for blank group ，AR model was prepared by sensitization and challenge with ovalbumin on day 0，7，14 and 21-27. Astragaloside Ⅳ group was given astragaloside Ⅳ 40 mg/kg intraperitoneally at the dose of 0.02 mL/g on the 15th to 27th day of modeling （given the drug 1 h before challenge sensitization on the 21st to 27th day ）. Blank group and model group were given constant volume of normal saline intraperitoneally ，once a day. Twenty-four hours after sensitization from the last challenge ， the infiltration of inflammatory cells in the nasal mucosa of each group was observed ，and the contents of interleukin 4（IL-4）， IL-5 and interferon gamma （IFN-γ）in the nasal lavage fluid were measured. The levels of reactive oxygen species （ROS），and the count of phosphorylated Janus kinase 2（p-JAK2）and phosphorylation signal transduction and activation of transcription protein 6 （p-STAT6）positive cells in the nasal mucosa and spleen as well as the phosphorylation levels of JAK 2 and STAT 6 proteins in spleen tissue （i.e. p-JAK 2/JAK2 ratio，p-STAT6/STAT6 ratio）were also determined. RESULTS ：Compared with blank group ，the number of inflammatory cells in the nasal mucosa （eosinophils and mast cells ）in the model group ，the contents of IL- 4 and IL- 5 in the nasal lavage fluid ，and the levels of ROS in the nasal mucosa and spleen tissues in the model group ，the count of p-JAK 2 and p-STAT 6 positive cells increased significantly ，the p-JAK2/JAK2 ratio，p-STAT6/STAT6 ratio in the spleen tissue were significantly increased （P＜0.05），and the content of INF-γ in the nasal lavage fluid was significantly decreased（P＜0.05）. Compared with model group ，the count of inflammatory cells infiltrated in the nasal mucosa ，the contents of IL- 4 and IL- 5 in the nasal cavity lavage fluid ，the level of ROS and the number of p-JAK 2 and p-STAT 6 positive cellsin the nasal mucosa and spleen tissue as well as the p-JAK2/JAK2 ratio and p-STAT 6/STAT6 ratio in spleen tissue were decreased significantly （P＜0.05），and the content of INF-γ in nasal lavage fluid was significantly increased（P＜0.05）. CO NCLUSIONS：Astragaloside Ⅳ can effectively improve the inflammatory response in AR model mice ，the mechanism of which may be related to down-regulation of JAK2/STAT6 signaling pathway and ROS level. |
| 期刊： | 2021年第32卷第10期 |
| 作者： | 陈旭青，马华安，周龙云，严道南，刘书芬，吴继勇 |
| AUTHORS： | CHEN Xuqing ，MA Huaan ，ZHOU Longyun ，YAN Daonan ，LIU Shufen ，WU Jiyong |
| 关键字： | 变应性鼻炎；黄芪甲苷；活性氧簇；Janus激酶2/信号转导及转录激活蛋白6信号通路；小鼠 |
| KEYWORDS： | Allergic rhinitis ；Astragaloside Ⅳ ；ROS； |
| 阅读数： | 297 次 |
| 本月下载数： | 3 次 |
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