金骨莲胶囊对炎症模型大鼠的抗炎作用及机制研究-中国药房

金骨莲胶囊对炎症模型大鼠的抗炎作用及机制研究

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篇名：	金骨莲胶囊对炎症模型大鼠的抗炎作用及机制研究
TITLE：
摘要：	目的：研究金骨莲胶囊对炎症模型大鼠的抗炎作用及机制。方法：将48只大鼠随机分为空白对照组、模型组、金骨莲胶囊低、中、高剂量组（0.66、1.32、2.64g/kg）和地塞米松组（阳性对照，0.945mg/kg），每组8只。空白对照组和模型组大鼠灌胃等体积水，其余各组大鼠灌胃相应药物，每天给药2次，连续3天。末次给药后30min，模型组和给药组大鼠腹腔注射脂多糖（10mg/kg）以复制炎症模型。腹腔注射6h后，于大鼠尾静脉取血，采用酶联免疫吸附试验检测大鼠血清中肿瘤坏死因子（TNF-α）、白细胞介素1β（IL-1β）、IL-6、前列腺素E（2PGE2）的含量；测定大鼠肺组织湿质量/干质量（W/D）比值；采用苏木精-伊红染色法观察大鼠肺组织的病理学形态变化；采用逆转录-聚合酶链式反应法检测大鼠肺组织中TNF-α、IL-6、PGE2、IL-1βmRNA的表达水平；采用Westernblot法检测大鼠肺组织中核因子κB（NF-κB）p65蛋白的磷酸化水平和NF-κB抑制蛋白α（IκBα）蛋白的表达水平。结果：与空白对照组比较，模型组大鼠血清中TNF-α、IL-1β、IL-6、PGE2的含量，肺组织W/D比值，肺组织中TNF-α、IL-1β、IL-6、PGE2mRNA的表达水平和NF-κBp65蛋白磷酸化水平均显著升高，IκBα蛋白表达水平显著降低（P＜0.05或P＜0.01）；大鼠肺组织中大量肺泡萎缩塌陷、壁增厚，可见肺实质化及大量炎症细胞浸润。与模型组比较，金骨莲胶囊各剂量组大鼠血清中TNF-α、IL-1β（低剂量组除外）、IL-6、PGE2的含量，以及肺组织中TNF-α（低剂量组除外）、IL-1β、IL-6（低、高剂量组除外）、PGE2mRNA的表达水平均显著降低（P＜0.05或P＜0.01）；金骨莲胶囊高剂量组大鼠肺组织W/D比值显著降低（P＜0.05或P＜0.01）；金骨莲胶囊中剂量组大鼠肺组织中NF-κBp65蛋白磷酸化水平均显著降低（P＜0.05或P＜0.01），IκBα蛋白表达水平显著升高（P＜0.05）；大鼠肺泡结构较为清晰、壁轻度增厚，并见少量炎症细胞浸润。结论：金骨莲胶囊对炎症模型大鼠具有良好的抗炎作用，其作用机制可能与抑制NF-κB信号通路有关。
ABSTRACT：	OBJECTIVE：To study the anti- inflammatory effect and mechanism of Jingulian capsule on inflammatory model rats. METHODS ：Totally 48 rats were randomly divided into blank control group ，model group ，Jingulian capsule low-dose ， medium-dose and high-dose groups （0.66，1.32，2.64 g/kg），dexamethasone group （positive control ，0.945 mg/kg），with 8 rats in each group. Blank control group and model group were given constant volume of water intragastrically ，and other groups were given relevant medicine intragastrocally ，twice a day ，for consecutive 3 days. Thirty minutes after last administration ，model group and administration groups were given lipopolysaccharide （10 mg/kg）intraperitoneally to induce inflammatory model. Six hours after intraperitoneal injection ，the contents of TNF-α，IL-1β，IL-6，PGE2 in serum were detected by ELISA. The wet to dry weight （W/D）ratio of lung tissue were determined. HE staining was used to observe the pathological changes of lung tissue . RT-PCR was used to detect the mRNA expression of TNF-α，IL-6，PGE2 and IL- 1β in lung tissue. Western blot assay was used to detect the phosphorylation of NF-κB p65 protein and the expression of IκBα protein in lung tissue. RESULTS：Compared with blank ； control group ，the contents of TNF-α，IL-1β，IL-6 and PGE 2 in serum ，the W/D ratio of lung tissue ，the expression of TNF-α，IL-1β，IL-6 and PGE 2 mRNA and the phosphorylation level of NF-κB p65 protein in lung tissue of model group weresignificantly increased ，and the expression of IκBα proteinwas significantly decreased （P＜0.05 or P＜0.01）；a large number of alveolar atrophy and collapse ，alveolar wall thickening ，lung consolidation ，and a large number of inflammatory cell infiltration could be seen in lung tissue. Compared with model group ，the contents of TNF-α，IL-1β（except for low-dose group ）， IL-6 and PGE 2 in serum ，as well as the expression of TNF-α（except for high-dose group ），IL-1β，IL-6（except for low-dose ， high-dose groups ）and PGE 2 mRNA in lung tissue were decreased significantly in Jingulian capsule groups （P＜0.05 or P＜0.01）； the W/D ratio of lung tissue was decreased significantly in Jingulian high-dose group （P＜0.05 or P＜0.01）；the expression of phosphorylation level of NF-κB p65 protein in lung tissue of Jingulian medium-dose group were decreased significantly （P＜0.05 or P＜0.01），while the expression of IκBα protein was increased significantly（P＜0.05）；the alveolar structure was clear ，the alveolar wall was slightly thickened ， and a small amount of inflammatory cell infiltration was seen in lung tissue. CONCLUSIONS：Jingulian capsule has good anti-inflammatory effect on inflammatory model rats ，the mechanism of which may be related to the inhibition of NF-κB signal pathway.
期刊：	2021年第32卷第09期
作者：	付志丽，蒲健，李龚路，谭丹，郑林，李勇军，黄勇
AUTHORS：
关键字：	金骨莲胶囊；抗炎作用；炎症因子；核因子κB信号通路
KEYWORDS：	Jingulian capsule ；Anti-inflammatory effect ；Inflammatory cytokines ；NF-κB signal pathway
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