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异黄绵马酸PB对红色毛癣菌生物被膜黏附及甾醇代谢相关酶基因表达的影响
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| 篇名： | 异黄绵马酸PB对红色毛癣菌生物被膜黏附及甾醇代谢相关酶基因表达的影响 |
| TITLE： | Study on Effects and Active Ingredients of Tibetan Medicine Lamiophlomis rotata against Rheumatoid Arthritis ZHANG Yanfei ，CHEN Ruixin ，YUAN Maohua ，YANG Zhizhong ，HUANG Ting ，CHEN Suxian ，JIANG Yunbin （578） Effects of Isoflavaspidic Acid PB on the Biofil |
| 摘要： | 目的：研究异黄绵马酸PB（简称“PB”）对红色毛癣菌生物被膜黏附及甾醇代谢相关酶基因表达的影响。方法：采用M38-A2法测定PB对红色毛癣菌的最小抑菌浓度（MIC）；采用MTT法筛选红色毛癣菌生物被膜的构建条件和初黏附时间；采用XTT法评价不同质量浓度PB（40、80、160µg/mL）对红色毛癣菌黏附过程的影响（另设置不加此药物的生长对照组，下同），并计算相对黏附率；采用XTT法并结合倒置显微镜观察不同质量浓度PB（20、40、80µg/mL）对不同初黏附时间下（3、5、9h）红色毛癣菌生物被膜形成的影响并计算黏附抑制率；采用实时荧光定量聚合酶链式反应法检测PB（320µg/mL）对红色毛癣菌生物被膜中甾醇代谢相关酶基因ERG6、ERG11mRNA表达的影响。结果：PB对红色毛癣菌的MIC为20µg/mL。红色毛癣菌生物被膜在含10％FBS的RPMI-1640培养基中且初黏附时间为6h时的代谢活性最强。与生长对照组比较，红色毛癣菌经不同质量浓度PB作用后，相对黏附率和生物被膜中ERG6、ERG11mRNA的相对表达量均显著降低（P＜0.01）；菌丝减少甚至消失，黏附抑制率（初黏附5、9h时）显著升高（P＜0.05或P＜0.01）。结论：PB能抑制红色毛癣菌的黏附过程，减少菌丝形成；该作用机制可能与抑制生物被膜中甾醇代谢相关酶基因ERG6、ERG11mRNA的表达有关。 |
| ABSTRACT： | OBJECTIVE：To study the effect of isofla vaspidicacid PB （called PB for short ）on the biofilm adhesion and the gene expression of ergosterol metabolism related enzymes in Trichophyton rubrum . METHODS ：M38-A2 method was adopted to determine MIC of PB to T. rubrum . MTT assay was used to screen the biolfilm condition and initial adhesion period of T. rubrum . The effects of different concentrations of PB （40，80，160 µg/mL）on the adhesion duration of T. rubrum （growth control group without PB was set up ，similarly hereinafter ）were evaluated and the adhesion rate was calculated by using XTT assay ；the effects of different concentrations of PB （20，40，80 µg/mL）on the biofilm formation of T. rubrum at different initial adhesion periods （3，5，9 h）were observed and the adhesion rate was calculated by using XTT assay combined with inverted microscope ；qRT-PCR method was used to detect the effects of PB （320 µg/mL）on the mRNA expression of ergosterol metabolism related enzyme gene ERG6 and ERG11 in biofilm of T. rubrum . RESULTS ：MIC of PB to T. rubrum was 20 µg/mL. The biofilm of T. rubrum in RPMI-1640 medium containing 10％ FBS was the most metabolism activity at 6 h of initial adhesion. Compared with growth control group ，after treated with different concentrations of PB ，adhesion rate and mRNA expression of ERG6 and ERG11 in biofilm were decreased significantly （P＜0.01）. Hyphae decreased or even disappeared ，and the adhesion inhibition rate （at 5 and 9 h of initial adhesion ）increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：PB can inhibit the adhesion of T. rubrum and reduce the hyphae ；the mechanism may be associated with the inhibition of the biofilm adhesion and mRNA expression of ergosterol metabolism related enzyme gene ERG6 and ERG11. |
| 期刊： | 2021年第32卷第05期 |
| 作者： | 侯捷，唐春萍，沈志滨，陈艳芬，丁慎，张祉思，李小英，江涛 |
| AUTHORS： | HOU Jie，TANG Chunping ，SHEN Zhibin ，CHEN Yanfen ，DING Shen，ZHANG Zhisi，LI Xiaoying ，JIANG Tao |
| 关键字： | 异黄绵马酸PB；红色毛癣菌；生物被膜；黏附；甾醇代谢 |
| KEYWORDS： | Isoflavaspidic acid PB ； Trichophyton |
| 阅读数： | 320 次 |
| 本月下载数： | 14 次 |
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