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一测多评法同时测定扶正固本颗粒中6种成分的含量
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| 篇名： | 一测多评法同时测定扶正固本颗粒中6种成分的含量 |
| TITLE： | Simultaneous Determination of the Contents of 6 Components in Fuzheng Guben Granules by QAMS |
| 摘要： | 目的：建立扶正固本颗粒中2，3，5，4′-四羟基二苯乙烯基葡萄糖苷、黄芩苷、淫羊藿苷、汉黄芩苷、黄芩素、汉黄芩素等6种成分含量的测定方法。方法：采用高效液相色谱法。色谱柱为DikmaDiamonsilC18，流动相为乙腈-0.1％磷酸水溶液（梯度洗脱），检测波长为275nm（0～8min）、320nm（8～9min）和275nm（9～33min），流速为1.0mL/min，柱温为25℃，进样量为10μL。以黄芩苷为基准物，采用多点校正法和斜率校正法分别计算另外5种成分的相对校正因子（fk/s），并采用保留时间差值法对待测成分进行色谱峰定位，比较上述两种一测多评法所得计算值与外标法实测值的差异。结果：2，3，5，4′-四羟基二苯乙烯基葡萄糖苷、黄芩苷、淫羊藿苷、汉黄芩苷、黄芩素、汉黄芩素检测进样量的线性范围分别为0.053～2.12、0.163～6.52、0.059～2.36、0.0216～0.864、0.03～1.2、0.021～0.84μg（r＞0.999），精密度、稳定性（12h）、重复性试验的RSD均小于3％；平均加样回收率为98.72％～99.82％（RSD为0.89％～1.24％，n＝9）。以黄芩苷为基准物，2，3，5，4′-四羟基二苯乙烯基葡萄糖苷、淫羊藿苷、汉黄芩苷、黄芩素、汉黄芩素多点校正法的fk/s分别为1.172、0.528、1.479、1.820、2.534，斜率校正法的fk/s分别为1.234、0.550、1.559、1.939、2.664；3种方法测得10批扶正固本颗粒中6种成分含量的RSD为0.29％～2.77％（n＝10）；两种一测多评法测得结果与外标法的Pearson相关系数均不低于0.9999（P＜0.001）。结论：成功建立了可用于同时测定扶正固本颗粒中6种成分含量的一测多评法。 |
| ABSTRACT： | OBJECTIVE：To establish the method for content determination of 6 components in Fuzheng guben granules ，such as 2，3，5，4′-tetrahydroxystilbene glucoside ，baicalin，icariin，scutellarin，baicalein and wogonin. METHODS ：HPLC method was adopted. The determination was performed on Dikma Diamonsil C 18 column with mobile phase consisted of acetonitrile- 0.1％ phosphoric acid aqueous solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelengths were set at 275 nm （0-8 min），320 nm（8-9 min）and 275 nm（9-33 min）. The column temperature was set at 25 ℃，and sample size was 10 μL. With baicalin as reference material ，the relative corr ection factors （fk/s） of other five components were calculated by multi-point correction method and slope correction method ；the retention time difference method was used to locate the chromatographic peaks ； the calculation values obtained by above 2 QAMS were compared with measured values of external standard method. RESULTS ： The linear range of 2，3，5，4′-tetrahydroxystilbene glucoside ，baicalin，icariin，scutellarin，baicalein and wogonin were 0.053-2.12， 0.163-6.52，0.059-2.36，0.021 6-0.864，0.03-1.2，0.021-0.84 μg（r＞0.999），respectively. RSDs of precision ，stability（12 h）and reproducibility tests were all lower than 3％. Average recoveries were 98.72％-99.82％（RSDs were 0.89％-1.24％，n＝9）. Using baicalin as reference material ，fk/s of multi-point correction method for 2，3，5，4′-tetrahydroxystilbene glucoside ，icariin，scutellarin， baicalein and wogonin were 1.172，0.528，1.479，1.820 and 2.534，respectively；fk/s of slope correction method were 1.234， 0.550，1.559，1.939，2.664. RSDs of 6 components in 10 batches of Fuzheng guben granules by 3 methods were 0.29％-2.77％ （n＝10），respectively. Pearson correlation coefficient was not lower than 0.999 9（P＜0.001）in measured values between QAMS and external standard method. CONCLUSIONS ：QAMS method is established successfully for simultaneous determination of 6 components in Fuzheng guben granules. |
| 期刊： | 2021年第32卷第02期 |
| 作者： | 仝立国，牛艳艳，王若瑜，吉海杰，宋美卿，冯玛莉，夏召弟，汪欣文 |
| AUTHORS： | TONG Liguo，NIU Yanyan ，WANG Ruoyu ，JI Haijie，SONG Meiqing ，FENG Mali，XIA Zhaodi ，WANG Xinwen |
| 关键字： | 一测多评法；扶正固本颗粒；2，3，5，4′-四羟基二苯乙烯基葡萄糖苷 ；黄芩苷；淫羊藿苷；汉黄芩苷；黄芩素；汉黄芩素； |
| KEYWORDS： | QAMS；Fuzheng guben granules ；2，3，5，4′-tetrahydroxystilbene glucoside ；Baicalin；Icariin；Scutellarin；Bai- |
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