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紫草素对人结肠癌细胞HCT116自噬和凋亡的影响
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| 篇名： | 紫草素对人结肠癌细胞HCT116自噬和凋亡的影响 |
| TITLE： | Effects of Shikonin on Autophagy and Apoptosis of Human Colon Cancer HCT 116 Cells |
| 摘要： | 目的：研究紫草素对人结肠癌细胞HCT116自噬和凋亡的影响。方法：以0（空白对照）、10、20、40μg/mL紫草素作用于HCT116细胞48h后，采用MTT法检测细胞增殖抑制率；采用流式细胞术检测细胞凋亡率；分别采用实时荧光定量-聚合酶链式反应法和Westernblotting法检测细胞中微管相关蛋白轻链3（LC3）和自噬相关蛋白Beclin-1、p62的mRNA及其蛋白表达水平。结果：与空白对照比较，10、20、40μg/mL紫草素作用48h后HCT116细胞的增殖抑制率和凋亡率均显著升高（P＜0.05或P＜0.01）。10、20、40μg/mL紫草素作用于HCT116细胞48h后均可不同程度地升高细胞中LC3、Beclin-1、p62mRNA及其蛋白的表达水平，除10μg/mL紫草素作用后细胞中LC3mRNA表达水平升高不显著外，其余指标水平与空白对照比较差异均有统计学意义（P＜0.05或P＜0.01）。结论：紫草素可诱导人结肠癌细胞HCT116发生凋亡，并激活其自噬途径。 |
| ABSTRACT： | OBJECTIVE：To study the effects of shikonin on autophagy and apoptosis of human colon cancer HCT 116 cells. METHODS：After treating HCT 116 cells for 48 h with shikonin at 0（blank control ）10，20，40 μg/mL，MTT method was used to detect inhibitory rate of cell proliferation. Flow cytometry was used to detect cell apoptosis rate. RT-qPCR assay and Western blotting were respectively used to detect mRNA and protein expressions of microtubule associated protein light chain 3（LC3）and autophagy-related protein Beclin- 1 and p 62. RESULTS ：Compared with blank control ，after treated with 10，20，40 μ g/mL shikonin for 48 h，proliferation inhibitory rate and apoptosis rate of HCT 116 cells were increased significantly （P＜0.05 or P＜ 0.01）. After treated with 10，20，40 μg/mL shikonin for 48 h，mRNA and protein expressions of LC 3，Beclin-1 and p 62 in HCT116 cells were increased to different extents ；except that mRNA expression of LC 3 was not increased significantly after treated with 10 μg/mL shikonin，the difference were statistically significant in other indexes ，compared with blank control （P＜0.05 or P＜0.01）. CONCLUSIONS ：Shikonin can induce the apoptosis of human colon cancer HCT 116 cells and activate its autophagy pathway. |
| 期刊： | 2021年第32卷第01期 |
| 作者： | 邵鑫，蒋先虹，王瑞，蒲强红，韩彬，刘福 |
| AUTHORS： | SHAO Xin，JIANG Xianhong，WANG Rui，PU Qianghong，HAN Bin，LIU Fu |
| 关键字： | 紫草素；人结肠癌细胞HCT116；自噬；凋亡 |
| KEYWORDS： | Shikonin；Human colon cancer HCT 116 cells；Autophagy；Apoptosis |
| 阅读数： | 289 次 |
| 本月下载数： | 16 次 |
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