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黄芪总皂苷对气虚模型大鼠的补气作用及机制研究
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| 篇名： | 黄芪总皂苷对气虚模型大鼠的补气作用及机制研究 |
| TITLE： | Study on “Qi-invigorating”Effect and Its Mechanism of Total Saponins of Astragalus membranaceus on Rats with Qi-deficiency |
| 摘要： | 目的：研究黄芪总皂苷对气虚模型大鼠的补气作用及可能机制，为阐明黄芪补气的药效物质基础提供参考。方法：将40只雄性Wistar大鼠按体质量随机分为正常组、模型组、阳性对照组[补中益气丸，4.5g（/kg·d），以生药量计]和黄芪总皂苷高、低剂量组[252、28g/（kg·d），以总皂苷量计]，每组8只。除正常组外，其余各组大鼠均采用“饮食不节+疲劳”法复制气虚模型。于造模同时，各给药组大鼠灌胃相应药物，正常组和模型组大鼠灌胃等体积水，每天1次，连续给药21d。末次给药后，观察大鼠的一般情况；检测大鼠的体质量、胸腺指数、脾指数；记录其负重力竭游泳时间；测定其脾T淋巴细胞亚群CD3、CD4，肝组织中三磷酸腺苷（ATP）、二磷酸腺苷（ADP），血清中白蛋白（ALB），全血中红细胞计数（RBC）、血红蛋白（HBG）以及血清中超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸、乳酸脱氢酶（LDH）、肌酸激酶（CK）、白细胞介素2（IL-2）、IL-12、肿瘤坏死因子α（TNF-α）的水平。结果：与正常组比较，模型组大鼠体质量、胸腺指数、脾指数、负重力竭游泳时间以及脾T淋巴细胞亚群CD3、ATP、ADP、ALB和IL-12水平均显著降低或缩短（P＜0.05或P＜0.01），MDA、乳酸、CK和TNF-α水平均显著升高（P＜0.05）。与模型组比较，黄芪总皂苷高剂量组大鼠的体质量、脾指数、负重力竭游泳时间以及T淋巴细胞亚群CD3、ATP、ADP、ALB、RBC水平均显著升高或延长（P＜0.05），MDA、乳酸、CK、TNF-α水平显著降低（P＜0.05或P＜0.01）；黄芪低剂量组大鼠的负重力竭游泳时间、ATP、ADP、IL-2水平均显著升高或延长（P＜0.05或P＜0.01），MDA、乳酸、CK、TNF-α水平均显著降低（P＜0.05或P＜0.01）。与阳性对照组比较，黄芪总皂苷高剂量组大鼠脾指数、脾T淋巴细胞亚群CD3水平、负重力竭游泳时间、ATP水平显著升高或延长（P＜0.05或P＜0.01），黄芪总皂苷高、低剂量组大鼠MDA水平均显著降低（P＜0.05或P＜0.01）。结论：黄芪总皂苷可通过减少机体乳酸的积累，降低CK活性、体内脂质过氧化物水平以及调节免疫，从而起到补气、延缓疲劳发生、增强运动能力的作用。 |
| ABSTRACT： | OBJECTIVE：To study “Qi-invigorating”effect and its possible mechanism of total saponins of Astragalus membranaceus on rats with Qi-deficiency ，and to provide reference for elucidating the material basis of “Qi-invigorating”effect of A. membranaceus . METHODS ：Forty male Wistar rats were randomly divided into normal group ，model group ，positive control group [Buzhong yiqi pills ，4.5 g/（kg·d）]，A. membranaceus total saponins high-dose and low-dose groups [ 252，28 g/（kg·d），by the amount of total saponins] according to body weight ，with 8 rats in each group. Except for normal group ，the model of Qi-deficiency was made in other groups by the method of “diet disorder+fatigue ”. At the same time ，administration groups were given relevant medicine intragastrically ，and normal group and model group were given constant volume of water，once a day ，for consecutive 21 days. After last administration ，the general situation of rats was observed ；the body weight ，spleen index and thymus index of rats were detected ；weight-bearing swimming time was recorded ；the levels of spleen T lymphocyte subsets CD 3 and CD 4，the levels of ATP and ADP in liver tissue ，serum levels of ALB ，RBC and HBG in blood as well as the serum levels of SOD，MDA，lactate，LDH，CK，IL-2，IL-12 and TNF-α were all detected. RESULTS：Compared with normal group ，body weight，thymus index ，spleen index ，weight-bearing swimming time ，the level of spleen T lymphocyte subsets CD 3，ATP，ADP， ALB，IL-2 and IL- 12 were decreased or shortened significantly in model group （P＜0.05 or P＜0.01）. The levels of MDA ， lactate，CK and TNF-α were increased significantly （P＜ 0.05）. Compared with model group ，body weight ，spleen index，weight-bearing swimming time ，the level of spleen T lymphocyte subsets CD 3 and the levels of ATP ，ADP，ALB， RBC and IL- 2 were increased significantly or prolonged（P＜0.05）；while the levels of MDA ，lactate，CK and TNF-α were decreased significantly in A. membranaceus total saponins high-dose group（P＜0.05 or P＜0.01）.Weight-bearing swimming time ，the levels of ATP ，ADP and IL- 2 in A. membranaceus total saponins low-dose group were increased significantly or prolonged （P＜0.05 or P＜0.01），while the levels of MDA ，lactate，CK and TNF-α were decreased significantly （P＜0.05 or P＜0.01）. Compared with positive control group ，spleen index ，spleen T lymphocyte subsets CD 3，weight-bearing swimming time and ATP level of A. membranaceus total saponins high-dose group were increased significantly or prolonged （P＜0.05 or P＜0.01），while MDA levels of A. membranaceus total saponins high-dose and low-dose groups were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：A. membranaceus total saponins can reduce the body ’s accumulation of blood lactic acid ，the activity of CK ，the level of lipid peroxide and regulate immunity to tonify Qi ，delay fatigue and improve exercise ability. |
| 期刊： | 2020年第31卷第24期 |
| 作者： | 张丹丹，王天合，余意，胡明华，李慧君，夏和元，罗心遥，杨玉莹，叶晓川 |
| AUTHORS： | ZHANG Dandan， WANG Tianhe，YU Yi，HU Minghua，LI Huijun，XIA Heyuan，LUO Xinyao，YANG Yuying，YE Xiaochuan |
| 关键字： | 黄芪总皂苷；补气作用；机制；气虚模型；大鼠 |
| KEYWORDS： | Astragalus membranaceus total saponins ；“Qi-invigorating”effect；Mechanism；Qi-deficiency model ；Rat |
| 阅读数： | 275 次 |
| 本月下载数： | 1 次 |
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