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紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响研究
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| 篇名： | 紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响研究 |
| TITLE： | Study on the Protective Effects of Butein on Oxidative Stress Injury of PC 12 Cell and Its Effects on Mitochondrial Function |
| 摘要： | 目的：研究紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响。方法：将大鼠PC12细胞分为正常对照组、模型组、溶剂对照组（1‰二甲基亚砜）和紫铆花素高、中、低浓度组（2、1、0.5μmol/L）。后4组给予相应试剂/药物干预24h后，除正常对照组外的其余组均以100mU/mL葡萄糖氧化酶诱导氧化应激模型。培养4h后，检测细胞存活率、凋亡率和细胞内活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、三磷酸腺苷（ATP）、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）水平或活性以及线粒体膜电位（MMP）变化。结果：与正常对照组比较，模型组细胞存活率和SOD、CAT、GSH-Px、ATP活性或水平均显著降低，凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著增加（P＜0.05或P＜0.01），MMP明显降低。与模型组比较，溶剂对照组上述指标均无明显变化（P＞0.05），紫铆花素高、中、低浓度组细胞存活率和SOD（除中、低浓度组外）、CAT、GSH-Px（除中、低浓度组外）、ATP（除低浓度组外）活性或水平均显著升高，凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著降低（P＜0.05或P＜0.01），MMP明显增加。结论：紫铆花素可通过增强抗氧化酶活性，稳定线粒体功能，抑制氧化应激和炎症反应，增加能量生成，进而抑制神经细胞凋亡，最终发挥神经保护的作用。 |
| ABSTRACT： | OBJECTIVE：To study the protective effects of butein on oxidative stress injury of PC12 cell and its effects on mitochondrial function. METHODS：Rats PC12 cells were divided into normal control group，model group，solvent control group（1 ‰ dimethyl sulfoxide），butein high，medium and low concentration groups（2，1，0.5 μmol/L）. The latter 4 groups were given relevant reagent/medicine for intervention；24 h later，other groups were given 100 mU/mL glucose oxidase to induce oxidant stress model except for normal control group. After 4 h culture，cell survival rate，apoptosis rate，the levels or activities of ROS，MDA，SOD，CAT，GSH-Px，ATP，IL-1β and TNF-α as well as the change of MMP were detected. RESULTS：Compared with normal control group，cell survival rate，the levels or activities of SOD，CAT，GSH-Px and ATP were all decreased significantly，and apoptotic rate，the content of ROS，the levels of MDA，IL-1β and TNF-α were all increased significantly（P＜0.05 or P＜0.01），while the MMP was decreased significantly. Compared with model group，above indexes of solvent control group had no significant change （P＞0.05），cell survival rates，the levels or activities of SOD （except for medium and low concentration groups），CAT，GSH-Px（except for medium and low concentration groups），ATP（except for low concentration group）were increased significantly in butein high，medium and low concentration groups，while apoptotic rates，the content of ROS，the levels of MDA，IL-1 β and TNF-α were decreased significantly（P＜0.05 or P＜0.01），while the MMP were increased significantly. CONCLUSIONS：Butein can increase the antioxidant enzyme activity， stabilize mitochondrial function， inhibit oxidative stress and inflammationthus， increase energy generation inhibiting neuronal cell apoptosis ultimately exerting a neuroprotective effect. |
| 期刊： | 2020年第31卷第24期 |
| 作者： | 刘珂娣，段佳林，苏晶，陶星茹，赵石，白杨，卫培峰，奚苗苗 |
| AUTHORS： | LIU Kedi，DUAN Jialin，SU Jing，TAO Xingru ，ZHAO Shi，BAI Yang，WEI Peifeng ，XI Miaomiao |
| 关键字： | 紫铆花素；PC12细胞；氧化应激；线粒体功能；炎症；细胞凋亡；神经保护 |
| KEYWORDS： | Butein； PC12 cell； Oxidative stress； Mitochondrial function；Inflammation；Apoptosis；Neuroprotection |
| 阅读数： | 286 次 |
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