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UPLC-Q-TOF-MS/MS分析苗药云实皮的化学成分
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| 篇名： | UPLC-Q-TOF-MS/MS分析苗药云实皮的化学成分 |
| TITLE： | Analysis of Chemical Compositions in Miao Medicine Caesalpinia decapetala by UPLC-Q-TOF-MS/MS |
| 摘要： | 目的：建立全面、快速分析苗药云实皮化学成分的方法，为该药质量控制和药效物质基础研究提供参考。方法：采用超高效液相色谱-四极杆-飞行时间串联质谱联用技术（UPLC-Q-TOF-MS/MS）。色谱柱为AgilentSB-C18，流动相为0.1％甲酸水溶液-0.1％甲酸乙腈溶液（梯度洗脱），流速为0.25mL/min，柱温为30℃，进样量为2µL；电喷雾离子源，正、负离子模式全程扫描，扫描范围为m/z50～1500，毛细管电压为4.5kV，雾化气（氮气）压力为1.2Bar，去溶剂气为氮气，去溶剂气流速为8L/min，去溶剂气温度为200℃。使用DataAnalysis4.2软件分析各成分峰的碎片离子信息，结合相关文献和对照品质谱图指认云实皮的化学成分。结果：在正离子模式下，共确定并指认9个化合物，峰1、2、3、4、5、6、7、8、9分别为儿茶素、原苏木素B、表儿茶素、没食子酸乙酯、槲皮素、木犀草素、3-去氧苏木查耳酮、异甘草素、亚油酸；在负离子模式下，共确定21个色谱峰，并指认了其中13个，峰3、4、5、6、7、8、9、10、11、12、13、15、21分别为儿茶素、短叶苏木酚酸、原苏木素B、表儿茶素、没食子酸乙酯、表儿茶素没食子酸酯、槲皮素、白藜芦醇、苏木查耳酮、木犀草素、3-去氧苏木查耳酮、异甘草素、亚油酸。结论：成功建立了可分析云实皮化学成分的UPLC-Q-TOF-MS/MS法。 |
| ABSTRACT： | OBJECTIVE：To estab lish a method that can comprehensively and rapidly analyze the chemical compositions of Miao medicine Caesalpinia decapetala，and to providing reference for quality control and pharmacodynamic material basis study of C. decapetala . METHODS ：UPLC-Q-TOF-MS/MS was adopted . The determination was performed on Agilent SB-C 18 column with mobile phase consisted of 0.1％ formic acid solution- 0.1％ formic acid acetonitrile （gradient elution ）at the flow rate of 0.25 mL/min. The column temperature was 30 ℃，and sample size was 2 µL. ESI source was applied in negative and positive scanning ion mode and data collection range of m/z 50-1 500. The capillary voltage was 4.5 kV，the atomizing gas （nitrogen）pressure was 1.2 Bar， the solvent removal gas was nitrogen ，the flow rate of solvent removal gas was 8 L/min and the solvent removal gas temperature was 200 ℃. Data Analysis 4.2 software was adopted to analyze fragment ion information of each peak ，and identify chemica l compositions on the basis of relevant literature and mass spectograms of reference substance. RESULTS ：Under positive ion mode ， 9 chemical compounds were identified ；peak 1，2，3，4，5，6，7，8，9 were catechin ，protohematoxylin B ，epicatechin，ethyl gallate，quercetin，luteolin，3-deoxy-hematoxylin chalcone ， isoliquiritigenin and linoleic acid. Under negative ion mode ， U1812403）， totally 21 peaks were confirmed and 13 compounds were identified；peak 3，4，5，6，7，8，9，10，11，12，13，15， 21 were catechins ， brevifolin carboxylic acid ， proto- hematoxylin B ，epicatechin，ethyl gallate ，epicatechin gallate ， quercetin，resveratrol，hematoxylin，luteolin，3-deoxy-hema- toxylin， isoliquiritigenin， linoleic acid. CONCLUSIONS UPLC-Q-TOF-MS/MS method is established successfully for analysis of chemical compositions in C. decapetala . |
| 期刊： | 2020年第31卷第20期 |
| 作者： | 罗媛，王昌权，巩仔鹏，李月婷，王爱民，陈思颖，黄勇，郑林 |
| AUTHORS： | LUO Yuan， WANG Changquan，GONG Zipeng，LI Yueting，WANG Aimin，CHEN Siying，HUANG Yong，ZHENG Lin |
| 关键字： | 苗药；云实皮；超高效液相色谱-四极杆-飞行时间串联质谱联用技术；化学成分；分析鉴定 |
| KEYWORDS： | Miao medicine ； Caesalpinia decapetala； UPLC-Q-TOF-MS/MS； Chemical composition ； Analysis and |
| 阅读数： | 331 次 |
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