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甘草次酸修饰多西紫杉醇磁性纳米粒的制备与表征
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| 篇名： | 甘草次酸修饰多西紫杉醇磁性纳米粒的制备与表征 |
| TITLE： | Preparation and Characterization of Glycyrrhetinic Acid-modified Docetaxel Magnetic Nanoparticles |
| 摘要： | 目的：制备甘草次酸修饰多西紫杉醇磁性纳米粒（GA-DTX-NGO/IONP-NPs），并对其理化性质进行评价。方法：以磁性纳米氧化石墨烯（NGO/IONP）作为抗肿瘤药物载体，多西紫杉醇（DTX）为模型药物，甘草次酸（GA）为靶头分子。采用水热法合成NGO/IONP、酰胺化反应合成GA修饰的壳聚糖（GA-CS）后，采用傅里叶红外光谱法、差示扫描量热法及振动样品磁测量法等对两者进行表征。采用离子凝胶化法制备GA-DTX-NGO/IONP-NPs；采用透射电镜、纳米粒度分析仪等对其微观形态、粒径及Zeta电位进行观察和测定；采用超滤离心法测定其包封率和载药量；通过观察有无外加磁场时的状态考察其磁性；结合808nm激光对其进行光热转换试验。结果：成功合成了NGO/IONP和GA-CS，且NGO/IONP呈现超顺磁性。GA-DTX-NGO/IONP-NPs在透射电镜下呈圆球状，粒径为（262.8±4.23）nm，Zeta电位为（13.6±1.51）mV，包封率为（94.29±0.50）％，载药量为（17.12±0.12）％。GA-DTX-NGO/IONP-NPs的外观呈黑色，分散均匀；其在外加磁场下磁性纳米粒可定向移动，显示出良好的磁定向性。在808nm激光照射下，GA-DTX-NGO/IONP-NPs具有良好的光热转换效应，且呈浓度和时间依赖趋势。结论：本研究成功制备了一种磁性纳米载药系统GA-DTX-NGO/IONP-NPs，可为肿瘤的磁热-化疗联合治疗提供一定的理论依据。 |
| ABSTRACT： | OBJECTIVE：To pre pare Glycyrrhetinic acid-modified docetaxel magnetic nanoparticles （GA-DTX-NGO/IONP- NPs），and to evaluate its physicochemical properties. METHODS ：Magnetic nano graphene oxide （NGO/IONP）was chosen as the anti-tumor drug carrier ，docetaxel（DTX）as the model drug and glycyrrhetinic acid （GA）as the target molecule. Firstly ，NGO/ IONP was synthesized by hydrothermal method and GA-CS was synthesized by amidation reaction. Fourier IR spectrometer ，DSC and vibration sample magnetic measuring instrument were used to characterize NGO/IONP and GA-CS. GA-DTX-NGO/IONP-NPs Huperzine A in the nicotinic acetylcholine receptor alleviates Aβ -induced 1-42 treatment of Alzheimer ’s disease and vascular dementia ：a neurotoxicity via downregulation of p 38 and JNK MAPK meta-analysis[J]. Evid Based Complement Alternat Med ， signaling pathways[J]. Neurochem Int ，2018. DOI ：10. 2014. DOI ：10.1155/2014/363985. 1016/j.neuint.2018.09.005. were prepared by the ion gelation method. TEM and particle size analyzer were used to observe and determine the morphology ， particle size and Zeta potential of GA-DTX-NGO/IONP-NPs ；the ultrafiltration-centrifugation method was used to determine encapsulation efficiency and drug loading amount ；the magnetic properties were investigated by investigating the state with or without external magnetic field ；the photothermal conversion test was carried out with laser irradiation of 808 nm. RESULTS ：NGO/ IONP and GA-CS were successfully synthesized ，and NGO/IONP exhibited superparamagnetism characteristics. GA-DTX-NGO/ IONP-NPs were spherical under TEM ，the particle size was （262.8±4.23）nm and the Zeta potential was （13.6±1.51）mV. The encapsulation rate and drug loading amount were （94.29±0.50）％ and（17.12±0.12）％，respectively. GA-DTX-NGO/IONP-NPs were black in appearance and evenly dispersed. Under the external magnetic field ，the magnetic nanoparticles could move directionally，showing good magnetic properties. GA-DTX-NGO/IONP-NPs showed a good concentration- and time-dependent photothermal conversion effect under 808 nm laser irradiation. CONCLUSIONS ：GA-DTX-NGO/IONP-NPs are successfully prepared. This study could provide some theoretical basis for the combined treatment of magnetic heating-chemotherapy for liver tumors. |
| 期刊： | 2020年第31卷第19期 |
| 作者： | 王莎莎，陈家琦，王华华，黄胜楠，贾永艳，祝侠丽 |
| AUTHORS： | WANG Shasha ，CHEN Jiaqi，WANG Huahua ，HUANG Shengnan ，JIA Yongyan ，ZHU Xiali |
| 关键字： | 磁性纳米氧化石墨烯；甘草次酸；多西紫杉醇；磁性纳米粒 |
| KEYWORDS： | Magnetic nano graphene oxide ；Glycyrrhetinic acid ；Docetaxel；Magnetic nanoparticles |
| 阅读数： | 281 次 |
| 本月下载数： | 6 次 |
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