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不同品种及采收期党参药材中5种有效成分含量的比较研究
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| 篇名： | 不同品种及采收期党参药材中5种有效成分含量的比较研究 |
| TITLE： | Comparative Study on Contents of 5 Active Ingredients in Different Varieties and Harvesting Periods of Codo- nopsis Radix |
| 摘要： | 目的：建立同时测定党参药材中党参炔苷、丁香苷、苍术内酯Ⅰ、苍术内酯Ⅱ和苍术内酯Ⅲ含量的方法，并比较不同品种及采收期党参药材中上述5种成分的含量。方法：采用高效液相色谱法（HPLC）。色谱柱为InertsilODS-3，流动相为乙腈-水（梯度洗脱），检测波长为210nm（党参炔苷）、220nm（苍术内酯Ⅱ、苍术内酯Ⅲ、丁香苷）、276nm（苍术内酯Ⅰ），流速为0.8mL/min，柱温为30℃，进样量为20μL。结果：党参炔苷、丁香苷、苍术内酯Ⅰ、苍术内酯Ⅱ和苍术内酯Ⅲ检测质量浓度的线性范围为44.30～886.00μg/mL（r＝0.9997）、6.50～130.03μg/mL（r＝0.9996）、4.47～89.46μg/mL（r＝0.9995）、2.53～50.50μg/mL（r＝0.9994）、5.64～112.80μg/mL（r＝0.9995）；定量限分别为2.4460、0.1680、0.2481、0.0657、0.0998μg/mL，检测限分别为1.3520、0.0672、0.0054、0.0063、0.0073μg/mL；精密度、稳定性（24h）、重复性、耐用性试验的RSD均小于2％；加样回收率分别为98.87％～100.62％（RSD＝0.73％，n＝6）、98.46％～101.54％（RSD＝1.15％，n＝6）、98.32％～101.12％（RSD＝1.19％，n＝6）、96.83％～104.16％（RSD＝2.62％，n＝6）、97.87％～100.99％（RSD＝1.07％，n＝6）。含量分别为33.78～431.82、0～20.60、0.44～3.68、0～10.83、0.27～73.40μg/g。党参炔苷含量由高到低为党参＞川党参＞素花党参＞洛党参；丁香苷为党参＞素花党参＞川党参，在洛党参中未检出；苍术内酯Ⅰ为素花党参＞洛党参＞党参＞川党参；苍术内酯Ⅱ为党参＞素花党参，在川党参和洛党参中未检出；苍术内酯Ⅲ为党参＞素花党参＞洛党参＞川党参。6～10月采收的党参药材中均以党参炔苷含量最高；6～10月苍术内酯Ⅰ含量均较低；8月苍术内酯Ⅱ未检出；9月苍术内酯Ⅰ、苍术内酯Ⅱ含量较低，且丁香苷、苍术内酯Ⅱ在部分样品中未检出。结论：所建HPLC法操作简单、准确、灵敏度高、重复性好，可用于同时测定党参药材中5种有效成分的含量；不同品种及采收期党参药材中5种有效成分含量存在较大差异。 |
| ABSTRACT： | OBJECTIVE：To establish a method for simultaneous determination of the contents of codonopatin ，syringin， atractylenolide Ⅰ，atractylenolide Ⅱ and atractylenolide Ⅲ，and to compare the contents of above 5 components in different varieties and harvesting periods of Codonopsis Radix. METHODS ：HPLC method was used. The column was Inertsil ODS- 3 with mobile phase consisted of acetonitrile-water （gradient elution ）at the flow rate of 0.8 mL/min. The detection wavelengths were 210 nm （codonopatin），220 nm （syringin，atractylenolide Ⅱ ，atractylenolide Ⅲ），276 nm （atractylenolide Ⅰ）. The column temperature was set at 30 ℃ ，and the sample size was 20 μ L. RESULTS：The linear range of codonopatin ，syringin， atractylenolide Ⅰ，atractylenolide Ⅱ and atractylenolide Ⅲ were 44.30-886.00 μg/mL（r＝0.999 7），6.50-130.03 μg/mL（r＝0.999 6）， 4.47-89.46 μg/mL（r＝0.999 5），2.53-50.50 μg/mL（r＝0.999 4），5.64-112.80 μg/mL（r＝0.999 5）；the limits of quantification were 2.446 0，0.168 0，0.248 1，0.065 7，0.099 8 μg/mL，and detection limits were 1.352 0，0.067 2，0.005 4，0.006 3，0.007 3 μ g/mL；RSDs of precision ，stability（24 h），repeatability and durability tests were all less than 2％；the recoveries were 98.87％-100.62％（RSD＝0.73％，n＝6），98.46％-101.54％ （RSD＝1.15％，n＝6），98.32％-101.12％（RSD＝1.19％，n＝ 96.83％-104.16％（RSD＝2.62％，n＝6），97.87％-100.99％ （RSD＝1.07％，n＝6）. The average contents were 33.78-431.82， 0-20.60，0.44-3.68，0-10.83，0.27-73.40 μ g/g. The content of 1271985629@qq.com codonopatin was in descending order was as follows as Codonopsis pilosula ＞C. tangshen ＞C. pilosula Nannf. var. modesta （Nannf.） L. T. Shen ＞ecotypic variety of C.·1677· tangshen. The content of syringin in descending order was C. pilosula ＞C. pilosula Nannf. var. modesta（Nannf.）L. T. Shen ＞C. tangshen，but it was not detected in ecotypic variety of C. tangshen . The content of atractylenolide Ⅰ in descending order was C. pilosula Nannf. var. modesta（Namf.）L. T. Shen ＞ecotypic variety of C. tangshen ＞C. pilosula ＞C. tangshen . The content of atractylenolide Ⅱ in C. pilosula was higher than C. pilosula Nannf. var. modesta（Nannf.）L. T. Shen ，but was no detected in C. tangshen and ecotypic variety of C. tangshen . The content of atractylenolide Ⅲ in descending order was C. pilosula ＞C. pilosula Nannf. var. modesta（Nannf.）L. T. Shen ＞ecotypic variety of C. tangshen ＞C. tangshen . In Codonopsis Radix collected from Jul. to Oct. ，the content of codonopatin was the highest ；the content of atractylenolide Ⅰ was lower in sample collected from Jun. to Oct.；atractylenolide Ⅱ was not detected in sample collected in Aug. ；the contents of atractylenolide Ⅰ and atractylenolide Ⅱ were the lower in sample collected in Sept. ，and syringin and atractylenolide Ⅱ were not detected in some samples. CONCLUSIONS ： The established HPLC method is simple ，accurate，highly sensitive and reproducible. It can be used to simultaneously determine 5 active ingredients contents of Codonopsis Radix ；there are great difference in contents of 5 active ingredients in different varieties and harvesting periods of Codonopsis Radix. |
| 期刊： | 2020年第31卷第14期 |
| 作者： | 刘付松，陈翠莎，孙佩，刘莎，韦中兰，刘小琪，吴发明 |
| AUTHORS： | LIU Fusong ，CHEN Cuisha ，SUN Pei，LIU Sha，WEI Zhonglan ，LIU Xiaoqi ，WU Faming |
| 关键字： | 党参；党参炔苷；丁香苷；苍术内酯Ⅰ；苍术内酯Ⅱ；苍术内酯Ⅲ；高效液相色谱法；品种；采收期；含量测定 |
| KEYWORDS： | Codonopsis Radix ；Codonopatin；Syringin；Atractylenolide Ⅰ；Atractylenolide Ⅱ；Atractylenolide Ⅲ；HPLC； |
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