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贵州产红孩儿药材的质量标准提升研究
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| 篇名： | 贵州产红孩儿药材的质量标准提升研究 |
| TITLE： | Study on Quality Standard Improvement of Rhizoma Begoniae from Guizhou |
| 摘要： | 目的：为提升贵州产红孩儿药材的质量标准提供参考。方法：收集5批贵州产红孩儿药材，观察药材粉末的显微特征，参照2015年版《中国药典》（四部）通则相关方法，采用薄层色谱法（TLC）对红孩儿药材进行定性鉴别并测定其水分、总灰分及水溶性浸出物的含量；采用高效液相色谱法测定红孩儿药材中芦丁的含量。结果：红孩儿药材粉末呈棕褐色，石细胞类方形、类多角形或不规则形；淀粉粒众多，复粒稀少；可见导管、草酸钙方晶/簇晶；在与红孩儿对照药材TLC图谱相同位置处显相同荧光斑点。5批红孩儿药材中水分含量为10.15％～11.41％，总灰分含量为8.70％～12.59％，水溶性浸出物含量为16.91％～19.58％。芦丁检测质量浓度的线性范围为18.47～147.8μg/mL（r＝0.9998），重复性、中间精密度、稳定性试验（8h）的RSD均小于3.0％，平均加样回收率为99.39％～100.29％（RSD为0.23％～2.59％，n＝3）；5批红孩儿药材中芦丁的含量为0.102％～0.198％。结论：药材水分、总灰分含量分别不得超过13.0％、14.0％，水溶性浸出物、芦丁含量分别不得低于15.0％、0.080％。本研究所建质量标准可用于红孩儿药材的质量控制。 |
| ABSTRACT： | OBJECTIVE：To provide reference for improving the quality standard of Rhizoma Bego niae from Guizhou . METHODS：Five batches of Rhizoma Begoniae from Guizhou were collected ，and the microscopic characteri stics of the Rhizoma Begoniae powder were observed. According to the corresponding methods in 2015 edition of Chinese Pharmacopoeia （part Ⅳ）， potent adenosine 50-triphosphate competitive phosphati - dylinositol-3-kinase/mammalian target of rapamycin inhibitors：discovery of compound 26（PKI-587），a highly effi cacious dual inhibitor Morpholine as a privileged structure ：a review on the me - dicinal chemistry and pharmacological activity of morpho - line containing bioactive molecules[J]. Med Res Rev ， qq.com 2019. DOI ：10.1002/med.21634. qualitative identification of Rhizoma Begoniae was conducted by TLC ，and the contents of moisture ，total ash and water-soluble extract in Rhizoma Begoniae were determined. The contents of rutin were determined by HPLC. RESULTS ：The powder of Rhizoma Begoniae medicinal materials was brown ，stone cells were square ，polygonal-like or irregular. There were many starch grains and few complex grains. The conduit ，calcium oxalate square crystal/cluster crystal were visible. The same fluorescence spots were found in the same location of TLC atlas of Rhizoma Begoniae control herb. The moisture ，total ash ，water-soluble extract contents were 10.15％-11.41％，8.70％-12.59％ and 16.91％-19.58％，respectively. The linear range of rutin were 18.47-147.8 μg/mL （r＝0.999 8）；RSDs of reproducibility ，intermediate precision and stability tests （8 h）were all lower than 3.0％；the average recoveries were 99.39％-100.29％（RSDs were 0.23％-2.59％，n＝3）；the contents of rutin in 5 batches of Rhizoma Begoniae were 0.102％-0.198％. CONCLUSIONS ：The contents of moisture and total ash shall not exceed 13.0％ and 14.0％ respectively， and the contents of water-soluble extract and rutin shall not be less than 15.0％ and 0.080％. The quality standard established in this study can be used for the quality control of Rhizoma Begoniae from Guizhou. |
| 期刊： | 2020年第31卷第12期 |
| 作者： | 刘利琴，彭潇，马雪，李勇军，刘春花，潘洁，王永林，王爱民 |
| AUTHORS： | LIU Liqin，PENG Xiao，MA Xue，LI Yongjun ，LIU Chunhua ，PAN Jie，WANG Yonglin ，WANG Aimin |
| 关键字： | 红孩儿；质量标准；显微观察；水分；总灰分；水溶性浸出物；芦丁；含量测定 |
| KEYWORDS： | Rhizoma Begoniae ；Quality standard ；Microscopic observation ；Moisture；Total ash ；Water-soluble extract ； |
| 阅读数： | 469 次 |
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