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钩吻总碱抑制人结肠癌细胞增殖及血管新生的作用研究
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| 篇名： | 钩吻总碱抑制人结肠癌细胞增殖及血管新生的作用研究 |
| TITLE： | Study on Inhibitory Effects of Total Alkaloids of Gelsemium elegans on the Proliferation and Angiogenesis of Human Colon Cancer Cells |
| 摘要： | 目的：探讨钩吻总碱（TAG）抑制人结肠癌细胞增殖及血管新生的作用。方法：体外培养人结肠癌细胞HT-29和人脐静脉内皮细胞（HUVEC），经低、中、高剂量TAG（40、80、120μg/mL）干预后，使用倒置荧光显微镜观察两种细胞的形态，采用CCK-8法检测两种细胞的存活率，采用流式细胞术检测HT-29细胞的周期变化情况，采用划痕试验、Transwell侵袭试验和管腔形成试验检测HUVEC的迁移率、侵袭率和管腔数量。结果：与空白组比较，TAG各剂量组HT-29细胞和HUVEC均有不同程度的减少，并可见死亡细胞，两者存活率均显著降低（P＜0.05或P＜0.01）；TAG各剂量组G2/M期HT-29细胞比例以及中剂量组G0/G1期细胞比例均显著升高，而TAG各剂量组S期细胞比例以及高剂量组G0/G1期细胞比例均显著降低（P＜0.05或P＜0.01）；TAG各剂量组HU-VEC的存活率、迁移率、侵袭率均显著降低，4～24h各时间点管腔数量均显著减少（P＜0.01）。结论：TAG可抑制人结肠癌HT-29细胞和HUVEC的增殖，可改变HT-29细胞的周期，并抑制HUVEC的迁移、侵袭及管腔形成。 |
| ABSTRACT： | OBJECTIVE：To investigate the inhibitory effects of total alkaloids of Gelsemium elegans （TAG） on the proliferation and angiogenesis of human colon cancer cells. METHODS ：Human colon cancer cell line HT- 29 and HUVEC were cultured in vitro . After the intervention of low- ，medium-，high-dose TAG （40，80，120 μg/mL），the morphology of the two cells was observed by fluorescence inversion microscope. The survival rate of HT- 29 cells and HUVEC was detected by CCK- 8 assay. Flow cytometry was used to detect HT- 29 cell cycle. The migration rate ，invasion rate and tube number of HUVEC were observed by scratching test ，Transwell invasion experiment and tube formation experiment. RESULTS ：Compared with blank group ，HT-29 cells and HUVEC were decreased to different extents in TAG groups ；dead cells were observed ，and the survival rate of both decreased significantly （P＜0.05 or P＜0.01）. The proportion of HT- 29 cells at G 2/M phase in TAG groups as well as those at G 0/G1 phase in medium-dose group were increased significantly ；the proportion of HT- 29 cells at S phase in TAG groups as well as those at G 0/G1 phase in high-dose group were decreased significantly （P＜0.05 or P＜0.01）. Survival rate ，migration rate and invasion rate of HUVEC were decreased significantly in TAG groups ，and tube number was also decreased significantly at each time point during 4-24 h（P＜0.01）. CONCLUSIONS ：TAG have inhibitory effect on the proliferation of human colon cancer HT- 29 cells and HUVEC，can change HT- 29 cell cycle ，inhibit the migration ，invasion and tube formation of HUVEC. |
| 期刊： | 2020年第31卷第08期 |
| 作者： | 王文义，檀兴慧，张萍萍，杨宇珂，黄梓泓，李德森，吴水生 |
| AUTHORS： | WANG Wenyi ，TAN Xinghui ，ZHANG Pingping ，YANG Yuke，HUANG Zihong ，LI Desen，WU Shuisheng |
| 关键字： | 钩吻总碱；结肠癌；HT-29细胞；人脐静脉内皮细胞；增殖；迁移；侵袭；血管新生 |
| KEYWORDS： | Total alkaloid of Gelsemium elegans ；Colon cancer ；HT-29 cells；HUVEC；Proliferation；Migration；Invasion； |
| 阅读数： | 236 次 |
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