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草果醇提物不同极性部位的体外抗氧化活性研究
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| 篇名： | 草果醇提物不同极性部位的体外抗氧化活性研究 |
| TITLE： | Study on in vitro Antioxidant Activities of Different Polar Parts of Ethanol Extract from Amomum tsao-ko |
| 摘要： | 目的：评价草果醇提物4种不同极性部位的体外抗氧化活性，为该植物中抗氧化活性化学成分的开发研究奠定基础。方法：将草果的干燥果实粉碎后，用95％乙醇加热回流提取，经旋转蒸发浓缩、水浴蒸干后得到醇提取物浸膏；将该浸膏以水分散，再依次用石油醚、乙酸乙酯、正丁醇进行萃取，分别合并各溶剂萃取液，并收集下层水相，再经旋转蒸发浓缩、水浴蒸干后得到石油醚部位、乙酸乙酯部位、正丁醇部位和水部位。采用经典体外抗氧化活性试验，以2，6-二叔丁基-4-甲基苯酚（BHT）作为阳性对照，考察草果醇提物不同极性部位对1，1-二苯基-2-三硝基苯肼（DPPH）自由基、超氧阴离子自由基的清除能力和对三价铁离子（Fe3+）的还原能力。结果：草果醇提物4种极性部位对DPPH自由基的清除率均达到80％以上，其清除能力强弱排序为乙酸乙酯部位＞BHT＞正丁醇部位＞石油醚部位＞水部位；对超氧阴离子自由基的清除率大部分约为30％～40％，其清除能力强弱排序为正丁醇部位＞石油醚部位＞水部位＞乙酸乙酯部位＞BHT，但整体弱于其对DPPH自由基的清除能力；对Fe3+也有一定的还原能力，其还原能力强弱排序总体上为正丁醇部位＞BHT＞乙酸乙酯部位＞石油醚部位＞水部位，但当水部位质量浓度为4.0μg/mL时其还原能力升至最强。结论：草果的不同极性部位均具有一定的体外抗氧化能力，但其在不同抗氧化活性试验中的抗氧化活性能力强弱排序并不一致；乙酸乙酯部位对DPPH自由基的清除能力最强，正丁醇部位对超氧阴离子自由基的清除能力和对Fe3+的还原能力最强。 |
| ABSTRACT： | OBJECTIVE：To evaluate in vitro antioxidant activities of 4 different polar parts of ethanol extract from Amomum tsao-ko，and to lay a foundation for the research and development of antioxidant chemical components in the plant. METHODS ： The dried fruits of A. tsao-ko were crushed ，then were hearted and reflux extracted with 95％ ethanol. The extraction fluid was concentrated by rotary evaporation and evaporated in water bath to obtain the ethanol extract. The extract was dispersed in water ， and then extracted with petroleum ether ，ethyl acetate and n-butanol organic solvents one by one. Each solvent extract was combined and the lower water phase were collected. Finally ，the petroleum ether part ，ethyl acetate part ，n-butanol part and water part were obtained，after rotary evaporation concentration and water bath evaporation. Through in vitro antioxidant activity tests ，using 2， 6-di-tert-butyl-4-methylphenol（BHT）as positive control ，DPPH radical ，superoxide anion radical scavenging ability and Fe 3+ reducing ability of different polar parts of ethanol extract from A. tsao-ko were investigated. RESULTS ：The scavenging rates of 4 polar parts of ethanol extract from A. tsao-ko on DPPH radical were all over 80％；the order of scavenging ability was ethyl acetate part＞BHT＞n-butanol part ＞petroleum ether part ＞water part. Those of the 4 polar parts to superoxide anion radical were between about 30％-40％ mostly；the order of scavenging ability was n-butanol part ＞petroleum ether part ＞water part ＞ethyl acetate part ＞ BHT；but those were weaker than their scavenging ability to DPPH r adical. The polar parts of ethanol extract also had a certain reduction ability to Fe 3+；the order of the reduction ability was n-butanol part ＞BHT＞ethyl acetate part ＞petroleum ether part ＞ water part on the whole ，but that of water part rose to the stron- gest when its concentration was 4.0 μg/mL. CONCLUSIONS： The different polar parts of ethanol extract from A. tsao-kom have certain in vitro antioxidant capacity ，but the order of antioxidant activity of different polar parts was not the same in different antioxidant activity tests ；ethyl acetate part has the 163.com strongest scave nging effect on DPPH radical ，n-butanol part has the strongest scavenging ability on superoxide anion radical and reducing ability on Fe 3+. |
| 期刊： | 2020年第31卷第08期 |
| 作者： | 陈石梅，黄比翼，黄锁义 |
| AUTHORS： | CHEN Shimei ，HUANG Biyi，HUANG Suoyi |
| 关键字： | 草果；醇提物；极性部位；抗氧化活性；体外 |
| KEYWORDS： | Amomum tsao-ko ；Ethanol extract ；Polar parts ；Antioxidant activity ；in vitro |
| 阅读数： | 506 次 |
| 本月下载数： | 7 次 |
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