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不同产地茯苓中总蛋白的含量差异比较
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| 篇名： | 不同产地茯苓中总蛋白的含量差异比较 |
| TITLE： | Comparison of Total Protein Content Difference of Poria cocos from Different Producing Areas |
| 摘要： | 目的：比较不同产地茯苓中总蛋白的含量差异。方法：以牛血清白蛋白为对照，0.4mol/L氢氧化钠溶液为提取溶剂，考马斯亮蓝G-250为显色剂，采用紫外分光光度法于595nm波长处测定茯苓中总蛋白含量，并通过聚类分析对34批（S1～S34）不同产地的茯苓进行分类。结果：牛血清白蛋白检测质量浓度的线性范围为1.45～17.40μg/mL（r＝0.9996）；精密度、稳定性（20min）、重复性试验的RSD均小于3％；加样回收率为100.14％～104.26％（RSD＝1.43％，n＝9）；34批不同产地茯苓中总蛋白的含量为0.3884％～1.1297％。聚类分析结果显示，34批茯苓中，湖北省英山县产茯苓（S2、S3）的总蛋白含量＞1％，归为一类；湖北、云南、安徽和湖南产茯苓（S1、S5～S10、S12、S13、S16、S17、S19～S21、S23～S25、S28、S30、S31）的总蛋白含量为0.6535％～0.9461％归为一类；剩余批次含量为0.3884％～0.6012％，归为一类。结论：所建方法可用于获苓中总蛋白的含量测定。34批不同产地茯苓总蛋白含量以湖北省英山县产茯苓最高，云南、安徽等地次之。 |
| ABSTRACT： | OBJECTIVE：Compare the difference of total protein content of Poria cocos from different producing areas. METHODs：Using bovine serum albumin （BSA） as control ，0.4 mol/L sodium hydroxide solution as exctraction solution ， Coomassie brilliant blue G- 250 as chromogenic reagent ，visible spectrophotometry at 595 nm was used to determine the contents of total protein of P. cocos ；cluster analysis was used to classify 34 batches（S1-S34）of P. cocos from different producing areas. RESULTS：The linear range of BSA was 1.45-17.40 μ g/mL （r＝0.999 6）. RSDs of precision ，stability （20 min） and reproducibility tests were all lower than 3％；recoveries were 100.14％-104.26％（RSD＝1.43％，n＝9）. The contents of total protein in 34 batches of P. cocos from different producing areas were 0.388 4％-1.129 7％. The results of cluster analysis showed that among 34 batches of P. cocos ，the total protein content of P. cocos produced in Yingshan county of Hubei province （S2，S3） was higher than 1％，clustered into one category ；the total protein contents of P. cocos produced in Hubei ，Yunnan，Anhui and Hunan（S1，S5-S10，S12，S13，S16，S17，S19-S21，S23-S25，S28，S30，S31）were 0.653 5％-0.946 1％，clustered into one categony，and the remaining batch content were 0.388 4％-0.601 2％，clustered into one category. CONCLUSIONS ：Established method is suitable for the content determination of total protein content of P. cocos . The protein content of P. cocos from Yingshan county of Hubei province is the highest ，followed by Yunnan and Anhui in 34 batches of P. cocos from different producing areas. |
| 期刊： | 2020年第31卷第06期 |
| 作者： | 罗心遥，刘俊，李慧君，王天合，杨玉莹，夏和元，叶晓川 |
| AUTHORS： | LUO Xinyao ，LIU Jun，LI Huijun ，WANG Tianhe ，YANG Yuying ，XIA Heyuan ，YE Xiaochuan |
| 关键字： | 茯苓；蛋白；含量测定；差异；聚类分析 |
| KEYWORDS： | Poria cocos ；Protein；Content determination；Difference；Cluster analysis |
| 阅读数： | 322 次 |
| 本月下载数： | 3 次 |
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