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金荞麦中优效组分的网络药理学研究
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| 篇名： | 金荞麦中优效组分的网络药理学研究 |
| TITLE： | Study on Network Pharmacology of Superior Components of Fagopyrum dibotrys |
| 摘要： | 目的：研究金荞麦中优效组分的药理作用及可能的分子机制。方法：基于网络药理学，以类药性（DL）＞0.18和生物利用度（OB）＞30％为标准，借助中药整合药理学计算平台（TCMSP）筛选金荞麦中的优效组分；利用药效基团匹配与潜在识别靶标数据库（PharmMapper）获取金荞麦优效组分的潜在作用靶点；利用生物学信息注释数据库（DAVID）对获得的靶点蛋白进行京都基因与基因组数据库（KEGG）信号通路分析和基因本体（GO）生物过程（包括生物过程、分子功能及细胞组分）富集分析（均以P＜0.05为标准）。结果：从金荞麦中共筛选出15种优效组分[如槲皮素、木犀草素、原矢车菊素B1、（+）儿茶素、β-谷甾醇等]和114个潜在作用靶蛋白[如雌二醇17-β-脱氢酶1、环腺苷酸（cAMP）特异性3，5-环磷酸二酯酶4D、维生素D3受体、尿苷胞苷激酶2、HRAS基因编码的三磷酸尿苷酶等]；涉及34条重要通路，如丝裂原活化蛋白激酶（MAPK）信号通路、血管内皮生长因子（VEGF）信号通路、趋化因子信号通路和胰岛素信号通路，其中与癌症相关的通路11条，与代谢相关的通路7条，与内分泌系统相关的通路4条；涉及的分子功能有类固醇激素受体活性、配体依赖的核受体活性、蛋白激酶活性等，细胞组分有细胞液、细胞碎片、可溶性部分等，生物过程有细胞凋亡的调节、有机物反应的过程、内源性刺激反应的过程等。结论：金荞麦可能通过MAPK信号通路、VEGF信号通路、趋化因子通路、胰岛素信号通路等发挥抗肿瘤、抗炎及调节糖脂代谢的作用。 |
| ABSTRACT： | OBJECTIVE：To study the pharmacological effects and possible molecular mechanism of the superior components in Fagopyrum dibotrys . METHODS ：Based on network pharmacology ，by selecting DL ＞0.18 and OB ＞30％ as criteria ，superior components of F. dibotrys were screened out ，using the traditional Chinese medicine integrated pharmacology （TCMIP）platform. Pharm Mapper database was utilized to obtain the potential targets of each components ；Kyoto gene and genome database （KEGG） signal pathway analysis and gene ontology （GO）bioprocess enrichment analysis were performed for target protein with DAVID database（all using P＜0.05 as criteria ）. RESULTS ：15 kinds of superior components [such as quercetin ，luteolin，procyanidin B 1， （+）catechin and β-sitosterol，etc.] and 114 target proteins （such as estradiol 17-β-dehydrogenase 1，cAMP specific 3，5-cyclic phosphodiesterase 4D，vitamin D 3 receptor，uridine cytidine kinase 2，urokinase triphosphate encoded by HRAS gene ，etc.）were screened out ，involving 34 important pathways ，like MAPK signaling pathway ，VEGF signaling pathway ，chemokine signaling pathway and insulin signaling pathway ；among them ，11 were cancer-related pathways ，7 were metabolic-related pathways ，4 were endocrine-related pathways. The involved molecular functions included steroid receptor activity ，ligand dependent nuclear receptor activity，protein kinase activity ，etc.；cell components included cell fluid ，cell fragment ，soluble part ，etc.；biological processes included the regulation of apoptosis ，the process of organic response ，the process of endogenous stimulation response ，etc. CONCLUSIONS：F. dibotrys may play anti-tumor effects ，anti-inflammatory effects ，regulation of glycolipid metabolism by acting on MAPK signaling pathway ，VEGF signaling pathway ，chemokine signaling pathway and insulin signaling pathway. |
| 期刊： | 2020年第31卷第05期 |
| 作者： | 颜学玲，时政，张云 |
| AUTHORS： | YAN Xueling ，SHI Zheng，ZHANG Yun |
| 关键字： | 金荞麦；优效组分；网络药理学；靶点蛋白；信号通路；分子机制 |
| KEYWORDS： | Fagopyrum dibotrys ； Superior components ； Network pharmacology ；Target proteins ； Signaling pathways ； |
| 阅读数： | 336 次 |
| 本月下载数： | 9 次 |
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