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蒿甲醚自微乳化给药系统对小鼠人脑胶质瘤皮下移植瘤的抑瘤作用研究
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| 篇名： | 蒿甲醚自微乳化给药系统对小鼠人脑胶质瘤皮下移植瘤的抑瘤作用研究 |
| TITLE： | Study on Anti-tumor Effects of Artemether Self-microemulsifying Drug Delivery System on Subcutaneous Trans- plantation of Human Glioma in Mice |
| 摘要： | 目的：研究蒿甲醚（ARM）自微乳化给药系统（SMEDDS）对人脑胶质瘤皮下移植瘤模型小鼠的抑瘤效果。方法：以人脑胶质细胞瘤细胞株SHG44接种，并以组织块移植传代，建立裸小鼠皮下移植瘤模型。分别于接种后的第5、10、15、20、25天时测量小鼠肿瘤组织体积，绘制肿瘤生长曲线，确定肿瘤快速增殖初期。取裸小鼠30只，同法建立皮下移植瘤模型，并于肿瘤快速增殖初期时分为对照组（生理盐水）、ARM混悬液组[60mg/（kg·d）]和ARM-SMEDDS低、中、高剂量组[10、20、30mg/（kg·d）]并灌胃给予生理盐水或相应药液，每日1次，连续给药30d。记录小鼠体质量变化及一般情况，测定肿瘤组织体积变化并计算相对肿瘤增殖率。结果：皮下移植肿瘤块组织后第10天左右即进入肿瘤快速增殖初期。给药期间各组小鼠一般情况正常，未见明显异常反应。自给药第10天起，ARM-SMEDDS各剂量组小鼠的肿瘤组织体积均较对照组显著缩小（P＜0.05）；自给药第15天起，ARM-SMEDDS各剂量组小鼠的肿瘤组织体积均较ARM混悬液组显著缩小（P＜0.05）。末次给药后，ARM-SMEDDS各剂量组小鼠的相对肿瘤增殖率均较ARM混悬液组均显著降低（P＜0.05）。结论：ARM-SMEDDS具有明显的抑制人脑胶质瘤增长的效果，抑瘤效果优于剂量更高的ARM混悬液剂型。 |
| ABSTRACT： | OBJECTIVE：To study the anti-tumor effect of artemether （ARM）self-microemulsifying drug delivery system （SMEDDS） on human glioma subcutaneously transplanted model mice. METHODS ：Human glioma cell line SHG 44 was inoculated and passed on to establish subcutaneous transplanted tumor model of nude mice. At the 5th，10th，15th，20th and 25th day after inoculation ，the tumor tissue volume was measured and the growth curve was drawn to confirm the initial stage of rapid tumor proliferation. Thirty nude mice was collected to establish subeutaneously transplanted tumor nude model ，and then divided into control group （normal saline ），ARM suspension group [ 60 mg/（kg·d）]，ARM-SMEDDS low-dose ，medium-dose and high-dose groups [ 10，20，30 mg/（kg·d）] at the initial stage of rapid tumor proliferation. They were given normal saline and relevant solution intragastrically once a day ，for consecutive 30 d. The weight change and general sibuation of mice were recorded. The change of tumor volume was determined and relative tumor proliferation rate was calculated. RESULTS ：The subcutaneously transplanted tumor tissue entered the initial stage of rapid tumor proliferation from the 10th day after transplantation. The general situation was normal ，and there was no obvious abnormal reaction in mice of each group during treatment. Since 10th day of administration，tumor tissue volume of mice in ARM-SMEDDS groups were shortened significantly than control group （P＜0.05）. At 15th day of administration ，tumor volume of mice in ARM-SMEDDS groups were shortened significantly than ARM suspension group（P＜0.05）. After last administration ，relative tumor proliferation rates of mice in ARM-SMEDDS groups were decreased significantly，compared with ARM suspension group （P＜0.05）. CONCLUSIONS ：ARM-SMEDDS show significant inhibitory effect on the proliferation of human glioma ，and are better than suspension with higher dosage. |
| 期刊： | 2020年第31卷第04期 |
| 作者： | 张亚红，王丽娟，林凤云，兰作平，甘淋玲 |
| AUTHORS： | ZHANG Yahong ，WANG Lijuan ，LIN Fengyun ，LAN Zuoping ，GAN Linling |
| 关键字： | 蒿甲醚；自微乳化给药系统；人脑胶质瘤细胞；皮下移植瘤；小鼠；抑瘤作用 |
| KEYWORDS： | Artemether；Self-microemulsifying drug delivery system ；Human glioma cells ；Subcutaneous transplanted tumor ； |
| 阅读数： | 418 次 |
| 本月下载数： | 5 次 |
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