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基于斑马鱼模型的西洋参提取物心脏保护作用研究
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| 篇名： | 基于斑马鱼模型的西洋参提取物心脏保护作用研究 |
| TITLE： | Study on the Heart Protective Effect of Panax quinquefolium Extract Based on Zebra Fish Model |
| 摘要： | 目的：研究西洋参提取物的心脏保护作用。方法：通过耐受性实验确定西洋参提取物的给药剂量以及特非那定的最优造模剂量后，将受精后24h（24hpf）的斑马鱼分为空白对照组（0.5％二甲基亚砜）、模型组（2.5μg/mL特非那定）、阳性对照组（2.5μg/mL特非那定+30μg/mL白藜芦醇）和不同质量浓度西洋参提取物组（2.5μg/mL特非那定+5、10、25μg/mL西洋参提取物，以生药量计）；孵育48h后，以斑马鱼心率、心脏静脉窦-动脉球（SV-BA）间距为指标，并计算其心脏损伤修复率，以考察西洋参提取物的心脏保护作用。另取24hpf的斑马鱼分为空白对照组、模型组、阳性对照组和20批不同产地西洋参提取物组（5μg/mL，以生药量计），同法给药和孵育48h后，测定其心率、心脏SV-BA间距，并计算其心脏损伤修复率和心脏损伤相对修复率，进一步验证西洋参提取物的心脏保护作用。结果：与空白对照组比较，模型组斑马鱼心率显著降低（P＜0.01），心脏SV-BA间距显著增大（P＜0.01）；与模型组比较，阳性对照组和5、10、25μg/mL西洋参提取物组斑马鱼心率显著升高（P＜0.01），5、10μg/mL西洋参提取物组斑马鱼心脏SV-BA间距显著缩短（P＜0.05或P＜0.01），其中5μg/mL西洋参提取物组斑马鱼的心脏损伤修复率达73.77％。验证实验显示，与模型组比较，20批不同产地西洋参提取物组斑马鱼心率均显著升高（P＜0.01），心脏SV-BA间距均显著缩短（P＜0.01）；其心脏损伤修复率在70.45％～85.78％之间，心脏损伤相对修复率在75.48％～98.12％之间。结论：西洋参提取物具有较好的心脏保护作用。 |
| ABSTRACT： | OBJECTIVE：To study the heart protective effect of Panax quinquefolium extract of Zebra fish. METHODS ：The drug dosage of P. quinquefolium extract and the optimal modeling dose of terfenadine were determined by tolerance test ；24 hours post fertilization （24 hpf），Zebra fish were divided into control group （0.5％ dimethyl sulfoxide ），model group （2.5 μg/mL terfenadine），positive control group （2.5 μ g/mL terfenadine + 30 μ g/mL resveratrol） and different mass concentration P. quinquefolium extract groups （2.5 μg/mL terfenadine+5，10，25 μg/mL P. quinquefolium extract，by raw material ）. 48 h after incubation，the heart rate and sinus venous-bulbus arteriosus （SV-BA）interval of Zebra fish were used as indexes ，and the heart injury repair rate was calculated to investigate the heart protection of P. quinquefolium extract. Another 24 hpf Zebra fish were divided into blank control group ，model group ，positive control group ，20 batches of P. quinquefolium extract from different producing area groups （5 μg/mL，by raw material ），with same administration route and 48 h of incubation. The heart rate ，SV-BA interval of heart was determined ，heart injury repair rate and heart injury relative repair rate were calculated ；the heart protective effect of P. quinquefolium extract was further validated. RESULTS ：Compared with blank control group ，heart rate was decreased significantly（P＜0.01），while SV-BA interval was increased significantly in model group （P＜0.01）. Compared with model group，heart rate of Zebra fish was increased significantly in positive control group and 5，10，25 μg/mL P. quinquefolium extract groups（P＜0.01），while SV-BA interval of Zebra fish was shortened significantly in 5，10 μg/mL P. quinquefolium extract groups （P＜0.05 or P＜0.01）；heart injury repair rate of Zebra fish in 5 μg/mL P. quinquefolium extract group reached 73.77％. In validation test ，compared with model group ，heart rate was increased significantly （P＜0.01），while SV-BA interval of Zebra fish was decreased significantly in 20 batches of P. quinquefolium extract groups （P＜0.01）；heart injury repair rate ranged 70.45％-85.78％，and heart injury relative repair rate ranged 75.48％-98.12％. CONCLUSIONS ：P. quinquefolium extract has good heart protective effects. |
| 期刊： | 2020年第31卷第03期 |
| 作者： | 吕婧，李晨，杨龙飞，高燕，赵渤年 |
| AUTHORS： | LYU Jing，LI Chen，YANG Longfei ，GAO Yan，ZHAO Bonian |
| 关键字： | 西洋参；斑马鱼；心脏保护；静脉窦-动脉球；间距；心率 |
| KEYWORDS： | Panax quinquefolium ；Zebra fish ；Heart protection ；Sinus venous-bulbus arteriosus ；Interval；Heart rate |
| 阅读数： | 412 次 |
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