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参附益心方对缺氧原代心肌细胞脂肪酸利用的影响
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| 篇名： | 参附益心方对缺氧原代心肌细胞脂肪酸利用的影响 |
| TITLE： | Effects of Shenfu Yixin Decoction on the Utilization of Fatty Acid in Primary Hypoxic Cardiomyocytes |
| 摘要： | 目的：探讨参附益心方对缺氧原代心肌细胞脂肪酸利用的影响及其可能机制。方法：取新生1～3d的SD大鼠心尖部组织，进行原代心肌细胞的分离、培养与鉴定，并将细胞随机分为正常组、模型组、辅酶Q10组（阳性对照，1×10－4mol/L）和参附益心方低、高剂量组（0.25、0.5mg/mL）。除正常组外，其余各组细胞均于5％O2、5％CO2、90％N2条件下培养6h以复制缺氧损伤模型。缺氧6h后，采用荧光素酶发光法检测各组细胞中三磷酸腺苷（ATP）的含量，采用Westernblotting法检测其脂肪酸转位酶（FAT/CD36）、过氧化物酶体增殖物激活受体（PPARα、PPARβ/δ）的表达情况。结果：与正常组比较，模型组细胞中ATP含量以及FAT/CD36蛋白的相对表达量均显著降低（P＜0.05）。与模型组比较，辅酶Q10组和参附益心方高剂量组细胞中ATP含量均显著升高，而辅酶Q10组细胞中FAT/CD36、PPARα蛋白，参附益心方高剂量组细胞中FAT/CD36蛋白以及参附益心方各剂量组细胞中PPARα、PPARβ/δ蛋白的相对表达量均显著降低（P＜0.05）。结论：参附益心方可通过抑制FAT/CD36、PPARα、PPARβ/δ蛋白的表达来抑制缺氧原代心肌细胞的脂肪酸利用，改善其能量代谢。 |
| ABSTRACT： | OBJECTIVE：To investigate the effects of Shenfu yixin decoction on the utilization of fatty acid in primary hypoxic cardiomyocytes and its potential mechanism. METHODS ：The apical tissue of neonatal SD rats with 1-3 days old were collected ， and the primary cardiomyocytes were isolated ，cultured and identified. The cardiomyocytes were randomly divided into normal group，model group ，coenzyme Q 10 group（positive control ，1×10－4 mol/L），Shenfu yixin decoction low-dose and high-dose groups（0.25，0.5 mg/mL）. Except for normal group ，cells in other groups were cultured under 5％O2，5％CO2 and 90％N2 for 6 hours to induce hypoxic injury model. After 6 hours of hypoxia ，the content of ATP was detected by luciferase luminescence assay. Western blotting assay was adopted to detect the expression of FAT/CD 36，PPARα and PPARβ/δ. RESULTS：Compared with normal group ，the content of ATP and relative expression of FAT/CD 36 protein were decreased significantly in model group （P＜ 0.05）. Compared with model group ，the content of ATP was increased significantly in coenzyme Q 10 group and Shenfu yixin decoction high-dose group ，while the relative expression of FAT/CD 36 and PPARα protein in coenzyme Q10 group，the relative expression of FAT/CD 36 protein in Shenfu yixin decoction high-dose group as well as the relative expression of PPARα and PPARβ/δ protein in Shenfu yixin decoction groups were decreased significantly （P＜0.05）. CONCLUSIONS ：Shenfu yixin decoction can inhibit the utilization of fatty acid of primary hypoxic cardiomyocytes and improve their energy metabolism by inhibiting the expression of FAT/CD 36，PPARα and PPARβ/δ protein. |
| 期刊： | 2020年第31卷第02期 |
| 作者： | 乔利杰，李彬，王新陆，谢世阳，高原，朱明军，王永霞 |
| AUTHORS： | QIAO Lijie，LI Bin，WANG Xinlu，XIE Shiyang ，GAO Yuan，ZHU Mingjun ，WANG Yongxia |
| 关键字： | 缺氧；原代心肌细胞；脂肪酸；代谢；参附益心方 |
| KEYWORDS： | Hypoxia；Primary cardiomyocytes ；Fatty acid ；Metabolism；Shenfu yixin decoction |
| 阅读数： | 309 次 |
| 本月下载数： | 6 次 |
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