目的：建立同时测定参芪颗粒中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花素含量的方法。方法：采用高效液相色谱法。

色谱柱为Welch Ultimate XB-C18，流动相为乙腈-0.2％甲酸溶液（梯度洗脱），检测波长为250 nm，柱温为35 ℃，流速为1.0 ml/min，

进样量为10 μl。结果：毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花素进样量线性范围分别为0.008～0.32、0.005～0.20、0.007～0.28

μg（r 为0.999 9、0.999 7、0.999 8）；精密度、稳定性、重复性试验的RSD≤1.59％；加样回收率范围分别为98.17％～99.71％、

98.03％～99.66％、98.16％～99.12％，RSD分别为0.67％、0.57％、0.43％（n＝6）。结论：该方法简便、准确、可靠，可用于参芪颗粒

中黄酮类成分的含量测定。

OBJECTIVE：To establish a method for simultaneous determination of the confents of calycosin-7-glucoside，calycosin

and formononetin in Shenqi granule. METHODS：HPLC was performed on the column of Welch Ultimate XB-C18 with mobile

phase of acetonitrile-0.2％ Formic acid solution（gradient elution）at flow rate of 1.0 ml/min，detection wavelength was 250 nm，

column temperature was 35 ℃，and injection volume was 10 μl. RESULTS：The linear range of sample quantity was 0.008-0.32 μg

for calycosin-7-glucoside（r＝0.999 9），0.005-0.20 μg for calycosin（r＝0.999 7）and 0.007-0.28 μg for formononetin（r＝0.999 8）；

RSDs of precsion，stability and reproducibility tests were no more than 1.59％；recoveries were 98.17％-99.17％（RSD＝0.67％，

n＝6），98.03％-99.66％（RSD＝0.57％，n＝6）and 98.16％-99.12％（RSD＝0.43％，n＝6）. CONCLUSIONS：The method is simple，

accurate and reliable，and can be used for the content determination of Shenqi granule ofiso flavones.