目的：探讨肺移植术后受体细胞色素P45（0 CYP）3A5和白介素（IL）-10基因多态性与他克莫司血药浓度/给药剂量（C/D）的

关系。方法：利用Sequenom MassArray iPLEX GOLD 系统检测51 例肺移植患者外周血CYP3A5 rs776746 和IL-10 rs1800896 位

点单核苷酸多态性，分析基因多态性与他克莫司代谢表型C/D的相关性。结果：肺移植术后，受体CYP3A5 rs776746 GG基因型患

者他克莫司C/D明显高于AA+AG型患者，差异有统计学意义（P＜0.01）。在术后第1 周和第2 周受体IL-10 rs1800896 AA基因型

患者他克莫司C/D明显高于AG基因型患者，差异有统计学意义（P＜0.05）。联合分析这两个基因，携带快代谢等位基因数目多的

患者他克莫司C/D 明显低于不携带的患者，差异有统计学意义（P＜0.01）。结论：联合检测受体CYP3A5 rs776746 和IL-10

rs800896 基因型有助于指导他克莫司个体化用药。

OBJECTIVE：To investigate the association of Cytochrome P4503A family polypeptide 5 [（CYP）3A5] and IL-10 polymorphisms

with the concentration/dose（C/D） ratio of tacrolimus in lung transplantation recipients. METHODS：CYP3A5 polymorphisms

at rs774746 and IL-10 polymorphisms at rs1800896 from peripheral blood cell of 51 lung transplant recipients was detected by Sequenom

MassArray iPLEX GOLD system. The relationship between gene polymorphism and tacrolimus C/D ratio was analyzed. RESULTS：

The tacrolimus C/D ratio of the recipients who carried CYP3A5 rs776746 GG genotype were higher than those with AA+GG

genotype，with statistical significance（P＜0.01）. The tacrolimus C/D ratio of the recipients who carried IL-10 rs1800896 AA genotype

were higher than those with AG genotype during 1 week and 2 weeks after transplantation，with statistical significance（P＜0.05）. Combined

analysis of these two genes，the tacrolimus C/D ratio of the recipients who carried more fast metabolic alleles were higher than

those who didn’t carry fast metabolic alleles，with statistical significance（P＜0.01）. CONCLUSIONS：Determination of recipients

CYP3A5 at rs 776746and IL-10 at rs800896 genotype may be performed prospectively to help individualizing tacrolimus dose regimen.