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菊花水提物的质量控制成分筛选及其网络药理学作用研究
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| 篇名： | 菊花水提物的质量控制成分筛选及其网络药理学作用研究 |
| TITLE： | |
| 摘要： | 目的：基于多成分代谢筛选菊花的质量控制成分，并研究其网络药理学作用。方法：制备菊花水提物样品溶液。选取1只大鼠，腹腔麻醉后取其空肠段灌流菊花水提物，采用双灌流采血法收集血浆样品1；另选3只大鼠灌胃菊花水提物，采用腹主动脉采血法收集血浆样品2。采用超高效液相色谱-串联质谱（UPLC-MS/MS）法分析菊花水提物及血浆样品成分，并鉴定菊花水提物经代谢后的原型入血成分。采用中药系统药理学数据库及分析平台（TCMSP）和Swiss Target Prediction数据库检索筛选原型入血成分的核心靶点，并利用DAVID数据库对核心靶点的相关通路进行富集，再根据拓扑参数筛选出质量控制成分，并采用Cytoscape 3.7.1 软件分析菊花质量控制成分的药理作用。结果：经UPLC-MS/MS分析后，菊花水提物中共鉴定27个化合物，其中鉴定出原型入血成分共12个。经网络药理学分析后共确定7个质量控制成分，分别是大波斯菊苷、芹菜素-7-O-葡萄糖醛酸苷、木犀草素、田蓟苷、芹菜素、橙皮素、金合欢素，其可通过作用于代谢通路、癌症相关通路、信号转导相关通路、脂肪细胞脂解调控作用通路等治疗癌症、心脑血管疾病、神经疾病等。结论：本研究筛选了菊花水提物的可能质量控制成分，并通过网络药理学初步明确其药理作用，可为菊花药材的利用提供新的思路。 |
| ABSTRACT： | OBJECTIVE： To screen the quality control components of Chrysanthemum morifolium based multiple component metabolism， and study its network pharmacology effect. METHODS： The water extract of C. morifolium was prepared. A total of one rats were selected， water extract of C. morifolium was perfused in jejunum segment after abdominal anesthesia； plasma sample 1 was collected by double perfusion collection. Other 3 rats were given water extract of C. morifolium intragastrically， and plasma sample 2 was collected by abdominal aorta blood collection. UPLC-MS/MS was used to analyze water extract of C. morifolium and plasma sample component， and prototype blood-entry component in water extract of C. morifolium was identified after metabolism. TCMSP and Swiss Target Prediction database were used to screen the core target of prototype blood-entry component. DAVID database was used to enrich the related pathways of core target. The quality control components were screened according to topological parameters. Cytoscape software was used to analyze pharmacological effect of quality control components of C. morifolium. RESULTS： After UPLC-MS/MS analysis， 27 compounds were identified in water extract of C. morifolium， among which there were 12 prototype blood-entry components. After network pharmacology analysis， 7 quality control components were identified， i.e. cosmosiin， apigenin-7-O-glucuronide， luteolin， tilianin， apigenin， hesperetin， acacetin. It was possible to treat cancer， cardiovascular and cerebrovascular diseases， and neurological diseases by acting on metabolic pathway， cancer related pathway， signal transduction related pathway， adipocyte lipolysis regulatory pathway， etc. CONCLUSIONS： The study screen the possible quality control components of water extract of C. morifolium. The theoretical pharmacological effect of it can be clarified through network pharmacology， which can provide a new idea for the utilization of C. morifolium. |
| 期刊： | 2019年第30卷第23期 |
| 作者： | 韩星，李雪岩，杨海洋，江晓泉，温浩然，冀艳华，汪国鹏，刘洋 |
| AUTHORS： | HAN Xing，LI Xueyan，YANG Haiyang，JIANG Xiaoquan，WEN Haoran，JI Yanhua，WANG Guopeng，LIU Yang |
| 关键字： | 多成分代谢；网络药理学；质量控制成分；菊花水提物；药理作用 |
| KEYWORDS： | Multiple component metabolism； Network pharmacology； Quality control component； Water extract of Chrysanthemum morifolium； Pharmacological effect |
| 阅读数： | 384 次 |
| 本月下载数： | 4 次 |
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