蒲桃不同药用部位乙醇提取物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用研究-中国药房

蒲桃不同药用部位乙醇提取物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用研究

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篇名：	蒲桃不同药用部位乙醇提取物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用研究
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摘要：	目的：比较蒲桃不同药用部位（根、茎、叶、种子、花和果肉）乙醇提取物对α-葡萄糖苷酶、α-淀粉酶活性的抑制作用。方法：以半数抑制浓度（IC50）为评价指标，阿卡波糖为阳性对照，采用体外抑制模型方法评价蒲桃不同药用部位乙醇提取物对α-葡萄糖苷酶（酵母菌源和小鼠小肠源）和α-淀粉酶活性的抑制作用，并采用酶促动力学与Lineweaver-Burk双倒数法分析作用最强的药用部位对α-葡萄糖苷酶和α-淀粉酶活性的抑制类型。结果：蒲桃不同药用部位乙醇提取物对酵母菌源α-葡萄糖苷酶活性抑制作用的强弱顺序为蒲桃种子＞蒲桃茎＞蒲桃叶＞蒲桃根＞蒲桃花＞蒲桃果肉＞阿卡波糖，对小鼠小肠源α-葡萄糖苷酶活性抑制作用的强弱顺序为蒲桃种子＞蒲桃茎＞蒲桃根＞蒲桃叶＞蒲桃花＞蒲桃果肉＞阿卡波糖，对α-淀粉酶活性抑制作用的强弱顺序为阿卡波糖＞蒲桃种子＞蒲桃茎＞蒲桃根＞蒲桃叶＞蒲桃果肉＞蒲桃花。其中，蒲桃种子乙醇提取物对酵母菌源α-葡萄糖苷酶、小鼠小肠源α-葡萄糖苷酶和α-淀粉酶活性的抑制作用[IC50分别为（6.64±0.24）、（32.77±2.46）和（41.18±1.63） μg/mL]显著强于其他药用部位，并且对α-葡萄糖苷酶活性的抑制作用显著强于阿卡波糖[对酵母菌源α-葡萄糖苷酶和小鼠小肠源α-葡萄糖苷酶的IC50分别为（2 833.33±5.48）、（1 304.21±6.45） μg/mL]（P＜0.05），但其对α-淀粉酶活性的抑制作用不及阿卡波糖[IC50为（27.27±1.24） μg/mL]（P＜0.05）。酶促动力学研究结果表明，蒲桃种子乙醇提取物对α-葡萄糖苷酶、α-淀粉酶的抑制作用均为可逆竞争性抑制类型。结论：在蒲桃根、茎、叶、种子、花和果肉等不同部位中，以蒲桃种子对α-葡萄糖苷酶、α-淀粉酶活性的抑制作用最强，具有开发成辅助降糖的药品或保健食品的价值。
ABSTRACT：	OBJECTIVE： To compare inhibitory effects of ethanol extract of different medicinal parts （root， stem， leaf， seed， flower and flesh） from Syzygium jambos on the activities of α-glycosidase and α-amylase. METHODS： Using half-inhibitory concentration value （IC50） as evaluation index， acarbose as positive control， inhibitory effects of ethanol extract of different medicinal parts from S. jambos on the activities of α-glycosidase （from yeast and small instestine in mice） and α-amylase were evaluated with in vitro inhibition model. The enzymatic dynamics and Lineweaver-Burk methods were used to analyze the inhibitory type of the best medicinal part on the activities of α-glycosidase and α-amylase. RESULTS： In the yeast α-glucosidase inhibitory activity test， the order of inhibitory activity was S. jambos seed＞S. jambos stem＞S. jambos leaf＞S. jambos root＞S. jambos flower＞S. jambos flesh＞acarbose. In the mice intestine α-glucosidase inhibitory activity test， the order of inhibitory activity was S. jambos seed＞S. jambos stem＞S. jambos root＞S. jambos leaf＞S. jambos flower＞S. jambos flesh＞acarbose. In the α-amylase inhibitory activity test， the order of inhibitory activity was acarbose＞S. jambos seed＞S. jambos stem＞S. jambos root＞S. jambos leaf＞S. jambos flesh＞S. jambos flower. Ethanol extract of S. jambos seed had the stronger inhibition activity against α-glucosidase from yeast，α-glucosidase from small intestine in mice and α-amylase than other medicinal parts [IC50 were（6.64±0.24），（32.77±2.46） and （41.18±1.63） μg/mL]. Ethanol extract of S. jambos seed had the stronger inhibition activity against α-glucosidase than acarbose [IC50 to α-glucosidase from yeast and α-glucosidase from small intestine in mice were （2 833.33±5.48），（1 304.21±6.45） μg/mL] （P＜0.05）. The inhibitory effect of ethanol extract from S. jambos on the activity of α-amylase was less than that of acarbose [IC50 was （27.27±1.24） μg/mL] （P＜0.05）. Enzymatic dynamics showed that the inhibitory type of ethanol extract from S. jambos seed on α-glucosidase and α-amylase were both reversible competitive inhibition. CONCLUSIONS： Among different parts of S. jambos such as root， stem， leaf， seed， flower and flesh， S. jambos seed shows the strongest inhibitory effects on the activities of α-glucosidase and α-amylase， which has the value of being developed for the treatment of diabetes or health food.
期刊：	2019年第30卷第23期
作者：	温正辉，凌梅娣，余思萍，庄远杯，罗晓东，潘增烽，林大都，张声源
AUTHORS：	WEN Zhenghui，LING Meidi，YU Siping，ZHUANG Yuanbei，LUO Xiaodong，PAN Zengfeng，LIN Dadu，ZHANG Shengyuan
关键字：	蒲桃；药用部位；乙醇提取物；α-葡萄糖苷酶；α-淀粉酶；酶促动力学
KEYWORDS：	Syzygium jambos； Medicinal part； Ethanol extract； α-glucosidase； α-amylase； Enzymatic dynamics
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