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3种根瘤菌对干旱和盐胁迫条件下穿心莲种子萌发的影响
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| 篇名： | 3种根瘤菌对干旱和盐胁迫条件下穿心莲种子萌发的影响 |
| TITLE： | |
| 摘要： | 目的：研究费氏中华根瘤菌、放射型根瘤菌和田菁茎瘤固氮根瘤菌对干旱和盐胁迫条件下穿心莲种子萌发的影响。方法：采用聚乙二醇6000（以下简称“PEG”）和氯化钠（NaCl）浸种处理，分别建立穿心莲种子的干旱胁迫和盐胁迫模型。采用培养皿纸上发芽法，考察不同浓度PEG（0.05、0.10、0.15、0.20、0.25 g/mL）和NaCl（50、100、150、200 mmol/L）对穿心莲种子萌发指标（发芽率、发芽势、发芽指数、活力指数）的影响；分别采用费氏中华根瘤菌、放射型根瘤菌和田菁茎瘤固氮根瘤菌对穿心莲种子进行预处理后，同法考察其在干旱胁迫或盐胁迫条件下的各项萌发指标。结果：选取0.15 g/mL PEG溶液和50 mmol/L NaCl溶液分别构建穿心莲种子干旱胁迫和盐胁迫模型。费氏中华根瘤菌预处理可显著提高干旱胁迫条件下穿心莲种子的各项萌发指标以及盐胁迫条件下的发芽指数和活力指数，但同时可显著降低盐胁迫条件下的发芽率和发芽指数（P＜0.05）；放射型根瘤菌处理可显著提高干旱胁迫条件下穿心莲种子各项萌发指标以及盐胁迫条件下的发芽势、发芽指数和活力指数（P＜0.05）；田菁茎瘤固氮根瘤菌预处理可显著提高干旱胁迫条件下穿心莲种子的发芽率、发芽指数和活力指数以及盐胁迫条件下的发芽率和发芽势（P＜0.05）。结论：3种根瘤菌处理均可在不同程度上改善穿心莲种子在干旱或盐胁迫条件下的发芽能力。本研究可为干旱条件下穿心莲的种植和栽培，以及将滩涂地区作为穿心莲的种植地提供技术支持。 |
| ABSTRACT： | OBJECTIVE： To study the effects of Sinorhizobium fredii， Rhizobium radiobacter and Azorhizobium caulinodans on germination of Andrographis paniculata seed under drought and salt stress condition. METHODS： Drought and salt stress models of A. paniculata seed were established with PEG-6000 （called “PEG” for short）and NaCl respectively. The effects of different concentrations of PEG （0.05， 0.10， 0.15， 0.20， 0.25 g/mL） and NaCl （50， 100， 150， 200 mmol/L） on the germination indicators （germination rate， germination potential， germination index， vigor index） of A. paniculata seed were investigated by the method of germination on dish paper. The seeds were soaked with S. fredii， R. radiobacter and A. caulinodans， and then germination indicators were investigated under the condition of drought and salt stress. RESULTS： Drought and salt stress models of A. paniculata seed were established with 0.15 g/mL PEG solution and 50 mmol/L NaCl solution， respectively. S. fredii pretreatment could significantly improve all the germination indicators of A. paniculata seed under drought stress condition， as well as germination index and vigor index of it under salt stress condition， but significantly reduce germination rate and germination index of it under salt stress condition （P＜0.05）. R. radiobacter treatment could significantly improve all the germination indicators of A. paniculata seed under drought stress condition， as well as germination potential， germination index and vigor index of it under salt stress condition （P＜0.05）. A. caulinodans pretreatment could significantly improve germination rate， germination index and vigor index of A. paniculata seed under drought stress condition， as well as germination rate and germination potential of it under salt stress condition （P＜0.05）. CONCLUSIONS： Three kinds of Rhizobia can improve germination ability of A. paniculata seed under drought or salt stress condition to different degrees. This research can provide technical support for planting and cultivation of A. paniculata under drought condition， and using saline-alkali soil as A. paniculata plantation area. |
| 期刊： | 2019年第30卷第22期 |
| 作者： | 石志棉，杜勤，苏雨苗，刘潇晗 |
| AUTHORS： | SHI Zhimian，DU Qin，SU Yumiao，LIU Xiaohan |
| 关键字： | 穿心莲；种子；萌芽；根瘤菌；干旱胁迫；盐胁迫 |
| KEYWORDS： | Andrographis paniculata； Seed； Germination； Rhizobia； Drought stress； Salt stress |
| 阅读数： | 286 次 |
| 本月下载数： | 2 次 |
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