牡丹皮炒炭前后颜色与有效成分含量的相关性分析
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篇名: 牡丹皮炒炭前后颜色与有效成分含量的相关性分析
TITLE:
摘要: 目的:研究牡丹皮炒炭前后颜色与有效成分含量的相关性,为牡丹皮和牡丹皮炭饮片的鉴别和质量评价提供参考。方法:使用色差仪测定牡丹皮炒炭前后饮片粉末颜色的色度空间参数[明度值(L*)、红绿分量值(a*)、黄蓝分量值(b*)及总色差值(E*ab)],采用高效液相色谱法测定牡丹皮和牡丹皮炭饮片粉末中10种有效成分(没食子酸、5-羟甲基糠醛、儿茶素、氧化芍药苷、芍药苷、槲皮素、苯甲酰芍药苷、异鼠李素、丹皮酚、山柰素)的含量,并应用SPSS 21.0软件对颜色的色度空间参数和有效成分含量进行相关性分析。结果:没食子酸含量与色度空间参数均无相关性(P>0.05);5-羟甲基糠醛含量与L*、b*、E*ab呈极显著性负相关(P<0.01),与a*呈极显著性正相关(P<0.01);芍药苷含量与L*、b*、E*ab呈极显著性正相关(P<0.01),与a*无相关性(P>0.05);氧化芍药苷含量与L*、E*ab呈极显著性正相关(P<0.01),与a*呈极显著性负相关(P<0.01),与b*无相关性(P>0.05);儿茶素、槲皮素、苯甲酰芍药苷、异鼠李素、丹皮酚、山柰素含量与L*、b*、E*ab均呈显著性正相关(P<0.05),与a*呈显著性负相关(P<0.05)。结论:色差技术可以量化牡丹皮和牡丹皮炭饮片的颜色,牡丹皮炒炭前后颜色与其有效成分含量具有显著的相关性。
ABSTRACT: OBJECTIVE: To study the correlation between the color of Moutan Cortex and the contents of effective components before and after carbonized, and to provide reference for identification and quality evaluation of Moutan Cortex and its charcoal piece decoction. METHODS: Using colorimeter determination of Moutan Cortex and Moutan Cortex charcoal decoction pieces powder chroma space parameter [lightness value (L*), red, green and component values (a*), yellow and blue component value (b*) and total color difference value (E*ab)], HPLC method for content determination of 10 effective components (gallic acid, 5-HMF, catechins, oxypaeoniflorin, paeoniflorin, quercetin, benzoyl paeoniflorin, isorhamnetin, paeonol, kaempferide) content in Moutan Cortex and Moutan Cortex charcoal decoction pieces. On this basis, the correlation between the content of effective components and the chromaticity space parameter of a color was studied by SPSS 21.0 software. RESULTS: There was no correlation between gallic acid content and the chromaticity space parameter of a color (P>0.05). There was an extremely significant negative correlation between 5-HMF content and L*, b*, E*ab (P<0.01), and an extremely significant positive correlation between 5-HMF content and a* (P<0.01). The content of paeoniflorin was positively correlated with L*, b* and E*ab, but not with a* (P>0.05). The content of oxidized paeoniflorin was positively correlated with L* and E*ab (P<0.01), and negatively correlated with a* (P<0.01), but not with b* (P>0.05). The content of catechins, quercetin, benzoyl paeoniflorin, isorhamnin, paeonol and kaempferol were positively correlated with L*, b* and E*ab (P>0.05), and negatively correlated with a* (P>0.05). CONCLUSIONS: Chromatic aberration technology can quantify the color of Moutan Cortex and its charcoal decoction pieces, and there is a significant correlation between the color of Moutan Cortex before and after processing and the content of its active ingredients.
期刊: 2019年第30卷第17期
作者: 陈昕,黄茂胜,孟江,王淑美
AUTHORS: CHEN Xin,HUANG Maosheng,MENG Jiang,WANG Shumei
关键字: 牡丹皮;牡丹皮炭;含量;颜色;相关性分析
KEYWORDS: Moutan Cortex; Moutan Cortex charcoal; Content; Color; Correlation analysis
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