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女贞子提取物对破骨细胞分化及成骨细胞增殖的影响
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| 篇名： | 女贞子提取物对破骨细胞分化及成骨细胞增殖的影响 |
| TITLE： | |
| 摘要： | 目的：探讨女贞子提取物对破骨细胞分化及成骨细胞增殖的影响。方法：以1α，25-二羟基维生素D3诱导兔原代骨髓细胞获取破骨细胞，经女贞子醇提物和水煎物低、中、高剂量（均为2、20、200 mg/L）处理后，采用抗酒石酸酸性磷酸酶（TRACP）活性测定法检测各组细胞裂解液中TRACP活性及细胞中TRACP阳性细胞数，采用甲苯胺蓝染色法检测各组细胞骨吸收陷窝数量及骨陷窝面积百分率。以L-抗坏血酸、β-甘油磷酸钠、地塞米松诱导小鼠颅顶前成骨细胞亚克隆14细胞获取成骨细胞，采用MTT法检测各组细胞的相对增殖率，采用碱性磷酸酶（APK）活性测定法检测各组细胞中APK活性。结果：经不同剂量女贞子提取物处理后，TRACP阳性细胞数、骨吸收陷窝数量及其面积均有不同程度的改变，其中女贞子醇提物各剂量组和水煎物高剂量组TRACP活性及阳性细胞数、骨吸收陷窝数量及面积百分率，以及女贞子水煎物中剂量组TRACP活性及阳性细胞数、骨陷窝面积百分率均显著降低或减少，而水煎物低剂量组细胞的骨吸收陷窝数量显著增多（P＜0.05或P＜0.01）；女贞子提取物低、中剂量组细胞的相对增殖率以及提取物各剂量组细胞的APK活性均显著升高，而女贞子提取物高剂量组细胞的相对增殖率均显著降低（P＜0.01）。结论：女贞子提取物可抑制破骨细胞的TRACP活性，改变破骨细胞的骨吸收功能和成骨细胞的增殖行为，并增加成骨细胞的APK活性。 |
| ABSTRACT： | OBJECTIVE： To investigate the effects of Ligustri lucidi Fructus extract on the differentiation of osteoclasts and the proliferation of osteoblasts. METHODS： Rabbit primary bone marrow cells were induced with 1a，25-Dihydroxyvitamin D3 to obtain osteoclasts. After treated with low-dose， medium-dose and high-dose of L. lucidi Fructus ethanol and water extract （both 2， 20， 200 mg/L）， TRACP activity determined method was used to detect the activity of TRACP in cell lysis solution and the number of TRACP positive cells. The number of celluar bone resorption lacunae and the percentage of bone lacunae area were detected by toluidine blue staining. Osteoblasts were obtained by L-ascorbic acid， β-sodium glycerophosphate and dexamethasone-induced subcloning 14 of cranial parietal anterior osteocytes in mice. MTT assay and APK activity assay were used to detect relative proliferation rate of cells and the activity of APK. RESULTS： After treated with different doses of L. lucidi Fructus extract， the number of TRACP positive cells， the number of bone resorption lacunae and their area were changed in varying degrees. TRACP activity， the number of its positive cells， the number of bone resorption lacunae and the percentage of bone lacunae area in different dosage groups of L. lucidi Fructus ethanol extract and high-dose of water extract； the TRACP activity， the number of its positive cells and the percentage of bone lacunae area in L. lucidi Fructus water extract medium-dose group were decreased significantly， while the number of bone resorption lacunae in L. lucidi Fructus water extract low-dose group was increased significantly （P＜0.05 or P＜0.01）. The relative proliferation rate of cells in L. lucidi Fructus extract low-dose and medium-dose groups and APK activity of cells in extract groups were significantly increased， while the relative proliferation rate of cells were decreased significantly in L. lucidi Fructus ethanol extract and water extract high-dose groups （P＜0.01）. CONCLUSIONS： L. lucidi Fructus extract can inhibit TRACP activity of osteoclasts， change the bone resorption function of osteoclasts and the proliferation behavior of osteoblasts and increase APK activity of osteoclasts. |
| 期刊： | 2019年第30卷第16期 |
| 作者： | 雷丽娟，杨晓琴，周英，王慧娟，陈丽珍，俸婷婷 |
| AUTHORS： | LEI Lijuan，YANG Xiaoqin，ZHOU Ying，WANG Huijuan，CHEN Lizhen，FENG Tingting |
| 关键字： | 女贞子；醇提物；水煎物；破骨细胞；成骨细胞；骨吸收；分化；增殖 |
| KEYWORDS： | Ligustri lucidi Fructus； Ethanol extract； Water decoction； Osteoclasts； Osteoblasts； Bone resorption； Differentiation； Proliferation |
| 阅读数： | 306 次 |
| 本月下载数： | 2 次 |
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