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七参连湿疹膏对特应性皮炎模型豚鼠的改善作用研究
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| 篇名： | 七参连湿疹膏对特应性皮炎模型豚鼠的改善作用研究 |
| TITLE： | |
| 摘要： | 目的：考察七参连湿疹膏对特应性皮炎（AD）模型豚鼠的改善作用，并探讨其可能机制。方法：将雌性豚鼠随机分为正常对照组，模型对照组，基质对照组（七参连湿疹膏基质，1 g/kg），七参连湿疹膏低、中、高剂量组（0.5、1、2 g/kg）和丁酸氢化可的松乳膏组（0.5 g/kg），每组10只。以卵清蛋白为抗原，激发豚鼠皮肤变态反应以复制AD模型。末次激发48 h后，正常对照组和模型对照组豚鼠涂以生理盐水，基质对照组豚鼠涂以七参连湿疹膏基质，各给药组豚鼠涂以相应药物；每天2次，连续14 d。记录各组豚鼠给药前后的红斑、水肿、抓痕等症状评分；采用苏木精-伊红染色法观察豚鼠皮肤组织形态学特征；采用TUNEL法检测豚鼠皮肤组织角质形成细胞凋亡情况；采用酶联免疫吸附测定法检测豚鼠皮肤组织干扰素γ（IFN-γ）水平。结果：与正常对照组比较，模型对照组和基质对照组豚鼠皮肤组织中均可见炎症细胞浸润、表皮角化过度、棘层增厚等症状，其红斑、水肿、抓痕评分及总评分和皮肤组织中IFN-γ水平均显著升高，角质形成细胞凋亡数均显著减少（P＜0.01）。与模型对照组和基质对照组比较，各给药组豚鼠上述症状均有不同程度的减轻，其中各给药组豚鼠给药后的红斑、水肿、抓痕评分及总评分以及七参连湿疹膏中、高剂量组和丁酸氢化可的松乳膏组豚鼠皮肤组织中IFN-γ水平均显著降低，且给药组上述评分均显著低于同组给药前（P＜0.05或P＜0.01）；各给药组豚鼠角质形成细胞凋亡数均显著增加（P＜0.05或P＜0.01）。结论：七参连湿疹膏对AD模型豚鼠具有一定的改善作用，这种作用可能与促进角质形成细胞凋亡、降低皮肤组织INF-γ水平有关。 |
| ABSTRACT： | OBJECTIVE： To investigate the improvement effects of Qishenlian eczema cream on the atopic dermatitis （AD） model guinea pigs， and to investigate its possible mechanism. METHODS： Female guinea pigs were randomly divided into normal control group， model control group， matrix control group （Qishenlian eczema cream matrix， 1 g/kg）， Qishenlian eczema cream low-dose， medium-dose and high-dose groups （0.5， 1， 2 g/kg） and Hydrocortisone butyrate cream group （0.5 g/kg）， with 10 guinea pigs in each group. Using ovalbumin as antigen， the skin allergy of guinea pigs was stimulated to induce AD model. Forty-eight hours after the last excitation， guinea pigs were smeared with normal saline in normal control group and model control group， while those were smeared with Qishenlian eczema cream matrix in matrix control group， and administration groups were treated with relevant medicine， twice a day， for consecutive 14 d. The scores of erythema， edema and scratch before and after administration were recorded in each group. The morphological characteristics of skin were observed by HE staining. TUNEL method was used to detect the apoptosis of keratinocytes in skin tissue. The level of IFN-γ in skin lesions was detected by ELISA. RESULTS： Compared with normal control group， inflammatory cell infiltration， hyperkeratosis of epidermis and thickening of spinous layer were observed in skin tissue of guinea pigs in model control group and matrix control group； the scores of erythema， edema and scratch and their total score， the level of IFN-γ in skin tissue were increased significantly； the apoptosis number of keratinocytes was decreased significantly （P＜0.01）. Compared with model control group and matrix control group， above symptoms of medication groups were relieved to different extents； the scores of erythema， edema and scratch and their total score after medication in medication groups， the level of IFN-γ in skin tissue in Qishenlian eczema cream medium-dose and high-dose groups and Hydrocortisone butyrate cream group were decreased significantly， and above scores in medication groups were significantly lower than before medication （P＜0.05 or P＜0.01）； the apoptosis number of keratinocytes was increased significantly in medication groups （P＜0.05 or P＜0.01）. CONCLUSIONS： Qishenlian eczema cream has certain improvement effect on AD model guinea pigs， the mechanism of which may be associated with promoting the keratinocyte apoptosis， reducing the level of INF-γ in skin tissue. |
| 期刊： | 2019年第30卷第12期 |
| 作者： | 姚荣成，刘韬，何波，杨仁华，沈志强，陈鹏 |
| AUTHORS： | YAO Rongcheng，LIU Tao2，HE Bo，YANG Renhua，SHEN Zhiqiang，CHEN Peng |
| 关键字： | 七参连湿疹膏；特应性皮炎；症状评分；角质形成细胞；干扰素γ；豚鼠；机制 |
| KEYWORDS： | Qishenlian eczema cream； Atopic dermatitis； Symptom score； Keratinocyte apoptosis； IFN-γ； Guinea pigs； Mechanism |
| 阅读数： | 400 次 |
| 本月下载数： | 7 次 |
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