Box-Behnken设计-响应面法优化芡实中总维生素E的提取工艺研究
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篇名: Box-Behnken设计-响应面法优化芡实中总维生素E的提取工艺研究
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摘要: 目的:优化芡实中总维生素E的提取工艺。方法:以芡实总维生素E提取量为考察指标,提取时间、提取次数、超声功率、粉碎度为考察因素,采用单因素试验和Box-Behnken设计-响应面法优化芡实总维生素E的提取工艺,并进行3次(芡实用量分别为2.0、20.0、40.0 g)验证试验。结果:芡实中维生素E的最佳提取工艺参数为提取时间80 min,提取次数3次,超声功率240 W,粉碎度80目。在验证试验中,总维生素E的提取量分别为2.063、2.103、2.085 mg/g(RSD分别为2.6%、1.5%、1.3%,n=3),与预测值(2.092 mg/g)的相对误差分别为0.14%、0.53%、0.33%。结论:优化的提取工艺的方法合理、稳定、可行,可用于芡实中总维生素E的提取。
ABSTRACT: OBJECTIVE: To optimize the extraction technology of total vitamin E in Euryale ferox. METHODS: With the extraction amount of total vitamin E as reference index, using extraction time, extraction times, ultrasound power and comminution degree as reference factors, single fator test and Box-Behnken design-response surface methodology was used to optimize the extraction technology of total vitamin E from E. ferox. The validation tests were conducted for 3 times (the amounts of E. ferox were 2.0, 20.0, 40.0 g). RESULTS: The optimal extraction technology of vitamin E included that extraction time of 80 min, extraction times of 3 times, ultrasound power of 240 W, comminution degree of 80 mesh. In validation test, extraction rates of total vitamin E were 2.063, 2.103, 2.085 mg/g (RSD=2.6%, 1.5%, 1.3%, n=3), the relative errors of which to predicted value (2.092 mg/g) were 0.14%, 0.53% and 0.33%, respectively. CONCLUSIONS: The optimal extraction technology is reasonable, stable and feasible, and can be used for the extraction of total vitamin E in E. ferox.
期刊: 2019年第30卷第11期
作者: 王红,吴启南,崔永伟,伍城颖,沈蓓,樊修和
AUTHORS: WANG Hong,WU Qinan,CUI Yongwei,WU Chengying,SHEN Bei,FAN Xiuhe
关键字: 芡实;维生素E;优化;提取工艺;Box-Behnken设计-响应面法
KEYWORDS: Euryale ferox; Vitamin E; Optimization; Extraction technology; Box-Behnken design-response surface methodology
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