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基于网络药理学的羌活-独活药对抗炎作用机制研究
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| 篇名： | 基于网络药理学的羌活-独活药对抗炎作用机制研究 |
| TITLE： | |
| 摘要： | 目的：预测羌活-独活药对抗炎活性成分及其作用机制。方法：根据口服生物利用度≥30％和类药性≥0.18的原则分别筛选羌活、独活的活性成分，通过中药系统药理学分析平台（TCMSP）对羌活、独活的潜在作用靶点进行预测和筛选，然后以“Anti-inflammatory”为关键词在人类基因数据库Genecards中检索炎症相关靶基因，并与羌活、独活中活性成分靶基因映射筛选出共同靶点，再利用Cytoscape 3.5.1软件建立活性成分-靶点网络。将筛选得到的靶点在相互作用基因/蛋白质搜索工具平台STRING V 10.5构建其靶蛋白相互作用（PPI）网络，并进行京都基因与基因组百科全书（KEGG）信号通路和基因本体（GO）富集分析，以研究其抗炎机制。结果：从羌活、独活药对中共筛选得到香豆素、β-谷甾醇、欧前胡素、紫花前胡苷等15个活性成分，作用于转录因子AP-1、磷脂酰肌醇激酶3r亚基、雌激素受体等49个靶点，主要涉及乙型肝炎、细胞凋亡等19条信号通路，参与炎症反应调节、前列腺素类生物合成等47个生物过程。结论：预测了羌活-独活药对活性成分多靶点、多通路、多生物过程的抗炎机制，为其进一步的抗炎机制研究指明了方向。 |
| ABSTRACT： | OBJECTIVE： To predict the anti-inflammatory active components and mechanism of couplet medicine of Notopterygium incisum-Angelica pubescens. METHODS： According to the principle of oral bioavailability≥30％ and drug- likeness≥0.18， active components of N. incisum and A. pubescens were screened； TCMSP was used to predict and screen the potential target of them. Using “Anti-inflammatory” as keyword， inflammatory related target genes were retrieved from human gene database Genecards. Common target was screened by mapping the target genes of active ingredients from couplet medicine of N. incisum-A. pubescens. The active ingredient-target network was established by using Cytoscape 3.5.1 software. The screened targets were used to construct the target protein interaction （PPI） network on the STRING V 10.5 platform. Its anti-inflammatory mechanism was studied by KEGG signaling pathway and GO biological enrichment analysis. RESULTS： Totally 15 active components such as coumarin， beta-sitosterol， ammidin， nodakenin were selected from couplet medicine of N. incisum-A. pubescens. Acting on 49 targets such as transcription factor AP-1， PI3-kinase subunit gamma， estrogen receptor， they mainly involved 19 signaling pathways such as hepatitis B and cell apoptosis， and were involved in 47 biological processes such as regulating inflammatory response and prostaglandin biosynthesis. CONCLUSIONS： The anti-inflammatory mechanism of active components of couplet medicine of N. incisum-A. pubescens on multi-target， multi-channel and multi-biological processes is predicted， and it points out the direction for further anti-inflammatory mechanism study. |
| 期刊： | 2019年第30卷第9期 |
| 作者： | 段凯旋，李跃文，刘和波，王博龙 |
| AUTHORS： | DUAN Kaixuan，LI Yuewen，LIU Hebo，WANG Bolong |
| 关键字： | 羌活；独活；药对；抗炎机制；靶蛋白相互作用；信号通路 |
| KEYWORDS： | Notopterygium incisum； Angelica pubescens； Couplet medicine； Anti-inflammatory mechanism； Target protein interaction； Signaling pathway |
| 阅读数： | 458 次 |
| 本月下载数： | 7 次 |
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