麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用及机制研究-中国药房

麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用及机制研究

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篇名：	麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用及机制研究
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摘要：	目的：研究麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用，并探究其可能的作用机制。方法：通过腋下接种Lewis肺癌细胞复制Lewis肺癌移植瘤小鼠模型。将60只小鼠随机分为模型组（生理盐水，每天灌胃1次）、顺铂组（6 mg/kg，每周腹腔注射1次）、麦门冬汤加减方组（20 g/kg，每天灌胃1次）和联合组（每周腹腔注射6 mg/kg顺铂1次+每天灌胃20 g/kg麦门冬汤加减方1次），每组15只。所有小鼠均连续给药2周。给药结束后，检测各组小鼠瘤质量和胸腺指数，苏木精-伊红（HE）染色后观察其瘤体组织病理变化，TUNEL法检测其瘤体细胞凋亡指数，Western blot法检测其瘤体组织中B细胞淋巴瘤2（Bcl-2）和促凋亡基因Bax蛋白表达。结果：与模型组比较，麦门冬汤加减方组、顺铂组和联合组小鼠瘤质量、Bcl-2蛋白表达量均显著降低（P＜0.05），胸腺指数、瘤体细胞凋亡指数、Bax蛋白表达量均显著升高（P＜0.05）；并且联合组小鼠瘤质量、Bcl-2蛋白表达量明显低于麦门冬汤加减方组和顺铂组（P＜0.05），胸腺指数、瘤体细胞凋亡指数、Bax蛋白表达量明显高于麦门冬汤加减方组和顺铂组（P＜0.05）。HE染色结果显示，麦门冬汤加减方组、顺铂组和联合组小鼠肿瘤细胞密度有所降低，肿瘤组织坏死区域增多，并且联合组小鼠的坏死区面积明显大于麦门冬汤加减方组和顺铂组。结论：麦门冬汤加减方可通过下调瘤组织中Bcl-2蛋白表达和上调Bax蛋白表达，从而抑制Lewis肺癌移植瘤模型小鼠肿瘤的生长。
ABSTRACT：	OBJECTIVE： To study the inhibitory effect of modified Maimendong decoction combined with cisplatin on Lewis lung cancer transplantation model mice， and to explore its potential mechanism. METHODS： Lewis lung cancer transplantation mice model was induced via subaxillary inoculation of Lewis lung cancer cells. 60 mice were randomly divided into model group （normal saline， once a day， i.g.）， cisplatin group （6 mg/kg， once a week， i.p.）， modified Maimendong decoction group （20 g/kg. once a day， i.g.） and combination group （cisplatin 6 mg/kg， once a week， i.p.+modified Maimengdong decoction， once a day， i.g.）， with 15 mice in each group. All mice were treated for consecutive 2 weeks. After medication， tumor weight and thymus index were detected； HE staining was used to observe the pathological change of tumor tissue. TUNEL was used to detect apoptotic index of tumor tissue. The protein expressions of Bcl-2 and Bax were detected by Western blot assay. RESULTS： Compared with model group， tumor weight and protein expression of Bcl-2 were decreased significantly in modified Maimengdong decoction， cisplatin group and combination group （P＜0.05）， and thymus index， tumor apoptotic index and the protein expression of Bax were increased significantly （P＜0.05）. Tumor weight and protein expression of Bcl-2 in combination group were significantly lower than modified Maimengdong decoction group and cisplatin group （P＜0.05）； thymus index， tumor apoptotic index and the protein expression of Bax were significantly higher than addition and subtraction of modified Maimengdong decoction group and cisplatin group （P＜0.05）. HE staining showed that the density of tumor cells was decreased to certain degree in modified Maimengdong decoction group， cisplatin group and combination group； the area of necrosis area in the combination group was significantly larger than in modified Maimendong decoction group and cisplatin group. CONCLUSIONS： Modified Maimendong decoction can inhibit the growth of Lewis lung cancer transplanted tumor in mice by down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.
期刊：	2019年第30卷第9期
作者：	方芳，吴万垠，杨春旭，宁雪坚，谢伶俐，斯韬
AUTHORS：	FANG Fang，WU Wanyin，YANG Chunxu，NING Xuejian，XIE Lingli，SI Tao
关键字：	麦门冬汤加减方；顺铂；Lewis肺癌；移植瘤；B细胞淋巴瘤2；小鼠
KEYWORDS：	Modified Maimendong decoction； Cisplatin； Lewis lung cancer； Transplanted tumor； Bcl-2； Mice
阅读数：	244 次
本月下载数：	3 次

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