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川菊止痛胶囊对偏头痛模型大鼠的改善作用及机制研究
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| 篇名： | 川菊止痛胶囊对偏头痛模型大鼠的改善作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究川菊止痛胶囊对偏头痛模型大鼠的改善作用及机制。方法：将SD大鼠随机分为正常组、模型组、化学药阳性对照组（佐米曲普坦片，0.004 05 g/kg）、中药阳性对照组（复方羊角颗粒，4.32 g/kg）和川菊止痛胶囊高、中、低剂量组（1.6、0.8、0.4 g/kg），每组10只。每天灌胃给药1次，连续给药5 d。末次给药30 min后，除正常组外，其余各组大鼠头颈部皮下注射硝酸甘油（10 mg/kg）复制偏头痛模型。以挠头次数为指标评价造模后2 h内（30 min为一个时间段）各组大鼠行为学变化；造模4 h后，采用全自动血液流变仪检测各组大鼠全血黏度（低切、中切、高切）、血浆黏度及红细胞聚集指数、红细胞刚性指数等血液流变学参数；酶联免疫吸附试验（ELISA）检测各组大鼠血清中一氧化氮（NO）、一氧化氮合成酶（NOS）、内皮素1（ET-1）、降钙素（CGRP）和脑组织中5-羟色胺（5-HT）、5-羟基吲哚乙酸（5-HIAA）、多巴胺（DA）、去甲肾上腺素（NE）水平。结果：与正常组比较，模型组大鼠在各时间段挠头次数显著增加（P＜0.01）；血清中NO、NOS和CGRP水平显著升高（P＜0.01），ET-1水平显著降低（P＜0.01）；脑组织中5-HT、DA和NE水平显著降低（P＜0.01），5-HIAA水平显著升高（P＜0.01）；全血黏度（低切）、红细胞聚集指数、红细胞刚性指数均显著升高（P＜0.05），血浆黏度显著降低（P＜0.01）。与模型组比较，化学药阳性对照组（0～120 min）、中药阳性对照组（60～90 min）和川菊止痛胶囊高（30～120 min）、中（60～90 min）剂量组大鼠挠头次数显著降低（P＜0.05或P＜0.01）；中药阳性对照组和川菊止痛胶囊高、中剂量组大鼠全血黏度（低切）和红细胞聚集指数显著降低（P＜0.05或P＜0.01）；化学药阳性对照组和川菊止痛胶囊高、中剂量组大鼠血清中NO、NOS、CGRP水平显著降低（P＜0.05或P＜0.01），ET-1水平显著升高（P＜0.01），脑组织5-HT、DA、NE水平显著升高（P＜0.05或P＜0.01），5-HIAA水平显著降低（P＜0.05）。结论：川菊止痛胶囊对偏头痛模型大鼠的改善作用与降低血清中NO、NOS、CGRP水平及升高脑组织中5-HT、DA、NE水平有关。 |
| ABSTRACT： | OBJECTIVE： To study the improvement effect and mechanism of Chuanju zhitong capsules （CZC） on migraine model rats. METHODS： SD rats were randomly divided into normal group， model group， chemical drug positive control group （Zolmitriptan tablet， 0.004 05 g/kg）， TCM positive control group （Compound yangjiao capsule， 4.32 g/kg）， CZC high-dose， medium-dose and low-dose groups （1.6， 0.8， 0.4 g/kg）， 10 rats in each group. All rats were relevant medicine intragastrically once a day， for successive 5 days. 30 min after last medication， except for normal group， rats in other groups were given glyceryl trinitrate subcutaneously （10 mg/kg） via head and neck to induce migraine model. The behavior changes of rats were evaluated using the times of scratching head as indexes within 2 h after modeling （30 min as a period of time）. 4 h after modeling， hemorheological parameters as whole blood viscosity （low-shearing， medium-shearing， high-shearing）， plasma viscosity， erythrocyte aggregation indexes and erythrocyte rigidity indexes were determined by automatic hemorheology instrument. The levels of NO， NOS， ET-1， CGRP in serum and the levels of 5-HT， 5-HIAA， DA and NE in cerebral tissue were determined by ELISA. RESULTS： Compared with normal group， the times of scratching head was increased significantly in model group at different periods （P＜0.01）. The serum levels of NO， NOS and CGRP were increased significantly （P＜0.01）， while ET-1 level was decreased significantly （P＜0.01）. The levels of 5-HT， DA and NE in cerebral tissue were decreased significantly （P＜0.01）， while 5-HIAA level was increased significantly （P＜0.01）. Whole blood viscosity （low-shearing）， plasma viscosity， erythrocyte aggregation index and erythrocyte rigidity indexes were increased significantly （P＜0.05），plasma viscosity were decreased significantly （P＜0.05） . Compared with model group， the times of scratching head were decreased significantly in TCM positive control group （0-120 min）， TCM positive control group （60-90 min）， CZC high-dose （30-120 min） and medium-dose （60-90 min） groups （P＜0.05 or P＜0.01）. The whole blood viscosity （low-shearing） and erythrocyte aggregation indexes of TCM positive control group， CZC high-dose and medium-dose groups were decreased significantly （P＜0.05 or P＜0.01）； the serum levels of NO， NOS and CGRP in chemical drug positive control group and CZC high-dose and medium-dose groups were decreased significantly （P＜0.05 or P＜0.01）， while the serum level of ET-1 was increased significantly （P＜0.01）； the levels of 5-HT， DA and NE were increased significantly in cerebral tissue （P＜0.05 or P＜0.01）， while the level of 5-HIAA was decreased significantly （P＜0.05）. CONCLUSIONS： The improvement effect of CZC on migraine model rats is associated with decreasing the serum levels of NO， NOS and CGRP and increasing the levels of 5-HT， DA and NE in cerebral tissue. |
| 期刊： | 2019年第30卷第7期 |
| 作者： | 李凤金，霍金海，张树明，王顺 |
| AUTHORS： | LI Fengjin，HUO Jinhai，ZHANG Shuming，WANG Shun |
| 关键字： | 川菊止痛胶囊；偏头痛；改善作用；血液流变学；大鼠 |
| KEYWORDS： | Chuanju zhitong capsule； Migraine； Improvement effect； Hemorheology； Rats |
| 阅读数： | 279 次 |
| 本月下载数： | 2 次 |
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