星点设计-响应面法优选高良姜的砂烫炮制工艺
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篇名: 星点设计-响应面法优选高良姜的砂烫炮制工艺
TITLE:
摘要: 目的:优选高良姜的砂烫炮制工艺,为高良姜的规范化炮制提供科学依据。方法:采用高效液相色谱法(HPLC)测定炮良姜中高良姜素和姜黄素含量。在单因素试验基础上,以炮制温度、炮制时间为考察因素,以高良姜素和姜黄素含量的综合评分为指标,采用星点设计-响应面法优选高良姜的砂烫炮制工艺,并进行工艺验证。结果:优选的工艺为炮制温度200 ℃、炮制时间5.5 min。3次验证试验中,平均综合评分为94.38(RSD=1.02%),与预测值93.74的相对误差为0.68%。结论:优选的炮制工艺方法简便、预测性良好,可用于高良姜的砂烫炮制。
ABSTRACT: OBJECTIVE:To optimize the hot sand processing technology of Alpinia officinarum,and to provide scientific evidence for the standardized processing of A. officinarum. METHODS:The contents of galangin and curcumin in processed A. officinarum were determined by HPLC. Based on single factor test,using processing temperature and processing time as factors,comprehensive score of galangin and curcumin contents as index,central composite design-response surface method was used to optimize hot sand processing technology of A. officinarum,and the processing technology was validated. RESULTS:The optimal processing technology included processing temperature of 200 ℃ and processing time of 5.5 min. In validation tests,average comprehensive score was 94.38 (RSD=1.02%),relative deviation of which to predicted value 93.74 was 0.68%. CONCLUSIONS:The optimized processing technology is simple and predictable. It can be used for hot sand processing technology of A. officinarum.
期刊: 2019年第30卷第7期
作者: 邓仙梅,黄斯敏,陈玉玲,孟江,赵斌
AUTHORS: DENG Xianmei,HUANG Simin,CHEN Yuling,MENG Jiang,ZHAO Bin
关键字: 高良姜;炮良姜;砂烫炮制;星点设计-效应面法;高良姜素;姜黄素
KEYWORDS: Alpinia officinarum; Processed Alpinia officinarum; Hot sand processing; Central composite design-response surface method; Galangin; Curcumin
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