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二氢槲皮素对局灶性脑缺血损伤模型大鼠血液流变学等相关指标的影响
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| 篇名： | 二氢槲皮素对局灶性脑缺血损伤模型大鼠血液流变学等相关指标的影响 |
| TITLE： | |
| 摘要： | 目的：观察二氢槲皮素（DHQ）对局灶性脑缺血损伤模型大鼠血液流变学等相关指标的影响。方法：将SD大鼠随机分为假手术组、模型组、尼莫地平组（阳性对照，20 mg/kg）和DHQ低、中、高剂量组（15、30、60 mg/kg），每组10只。各给药组大鼠均灌胃相应药物，假手术组大鼠灌胃等体积0.4％羧甲基纤维素钠溶液，每天1次，连续14 d。末次给药后，除假手术组外的其余各组大鼠均采用传统双侧颈总动脉结扎法复制局灶性脑缺血损伤模型。缺血24 h后，观察各组大鼠脑组织病理学变化，并检测其血液流变学指标[全血黏度（低、中、高切）、全血还原黏度（低、中、高切）、血浆黏度]、红细胞参数[红细胞压积、红细胞聚集指数（EAI）、红细胞变性指数（DI）、红细胞刚性指数（IR）]、凝血功能指标[活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、凝血酶时间（TT）、血浆纤维蛋白原（FIB）]水平。结果：与假手术组比较，模型组大鼠脑组织细胞疏松，间隙明显，缺血区周围神经元损伤明显，且全血黏度、全血还原黏度、血浆黏度、红细胞压积、EAI、IR、FIB水平均显著升高，DI、APTT、PT、TT均显著降低或缩短（P＜0.05或P＜0.01）。与模型组比较，各给药组大鼠上述症状均有不同程度的改善，且尼莫地平组大鼠全血黏度、血浆黏度、EAI、IR，DHQ高剂量组大鼠全血黏度、红细胞积压，DHQ各剂量组大鼠血浆黏度、EAI，DHQ中、高剂量组大鼠IR均显著降低；尼莫地平组大鼠DI、APTT、PT、TT，DHQ各剂量组大鼠DI、APTT、TT，DHQ高剂量组大鼠PT均显著升高或延长（P＜0.05或P＜0.01）。而其余指标组间比较，差异均无统计学意义（P＞0.05）。结论：DHQ对局灶性脑缺血损伤模型大鼠具有一定的保护作用，其作用机制可能与改善其血液流变学指标以及凝血功能异常有关。 |
| ABSTRACT： | OBJECTIVE： To observe the effects of dihydroquercetin （DHQ） on hemorheology and other relevant related indexes in local cerebral ischemic injury model rats. METHODS： SD rats were randomly divided into sham operation group， model group， nimodipine group （positive control， 20 mg/kg） and DHQ low-dose， medium-dose and high-dose groups （15， 30， 60 mg/kg）， with 10 rats in each group. Administration groups were given relevant medicine intragastrically， sham operation group and model group were given constant volume of 0.4％ Sodium carboxymethyl cellulose solution， once a day， for consecutive 14 d. After last administration， local cerebral ischemic injury model was induced by bilateral common carotid artery ligation in other groups except for sham operation group. After 24 h of cerebral ischemia， histopathological changes of brain tissue in rats of each group were observed； the levels of hemorheology indexes [whole blood viscosity （low， medium and high shear）， whole blood reduced viscosity （low， medium and high shear）， plasma viscosity]， erythrocyte parameters （hematocrit， EAI， DI， IR）， coagulation function indexes （APTT， PT， TT， FIB） were detected. RESULTS： Compared with sham operation group， the cells in the brain tissue of model group were loose， the gap was obvious， and the neurons around the ischemic area were damaged obviously； the levels of whole blood viscosity， whole blood reduced viscosity， plasma viscosity， hematocrit， EAI， IR and FIB were increased significantly， while the levels of DI， APTT， PT and TT were decreased or shortened significantly （P＜0.05 or P＜0.01）. Compared with model group， above symptoms of administration groups were improved to different extents， whole blood viscosity， plasma viscosity， EAI and IR of nimodipine group， whole blood viscosity and hematocrit of DHQ high-dose group， plasma viscosity and EAI of DHQ groups， and IR of DHQ medium-dose and high-dose groups were decreased significantly； DI， APTT， PT and TT of nimodipine group， DI， APTT and TT of DHQ groups and PT of DHQ high-dose group were increased or prolonged significantly （P＜0.05 or P＜0.01）. There was no statistical significance in other indexes among those groups （P＞0.05）. CONCLUSIONS： DHQ can protect against local cerebral ischemic injury model rats， the mechanism of which may be associated with improving hemorheology indexes and coagulation function disorder. |
| 期刊： | 2019年第30卷第6期 |
| 作者： | 顾媛媛，姜波，田明，韩玉生，刘旭，尚艳琦，梁慧，董晓红，代巧妹，周忠光 |
| AUTHORS： | GU Yuanyuan，JIANG Bo，TIAN Ming，HAN Yusheng，LIU Xu，SHANG Yanqi，LIANG Hui，DONG Xiaohong，DAI Qiaomei，ZHOU Zhongguang |
| 关键字： | 二氢槲皮素；血液流变学；红细胞参数；凝血功能；局灶性脑缺血损伤模型；大鼠 |
| KEYWORDS： | Dihydroquercetin； Hemorheology； Erythrocyte parameters； Coagulation function； Local cerebral ischemia injury model； Rats |
| 阅读数： | 388 次 |
| 本月下载数： | 7 次 |
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