石菖蒲中生物碱成分的分离与鉴定
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篇名: | 石菖蒲中生物碱成分的分离与鉴定 |
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摘要: | 目的:分离并鉴定石菖蒲的生物碱成分,为进一步研究其药理活性奠定基础。方法:利用阳离子交换树脂分离石菖蒲的生物碱成分;采用超高效液相色谱串联质谱(UPLC-MS)法进行鉴定,色谱柱为Agilent Zorbax Eclipse Plus-C18,流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),流速为0.3 mL/min,柱温为30 ℃,进样量为1 μL;离子源为电喷雾离子源,采用正离子模式检测,并结合Peakview 1.2等软件对生物碱成分进行分析。结果:石菖蒲中共检测到86个成分,并鉴定出5个生物碱成分,分别为菖蒲碱甲、N-反式阿魏酰酪胺、N-月桂基二乙醇胺、4-羟双氢鞘氨醇和5-丁基尿苷。结论:该试验结果可为石菖蒲生物碱成分的药理活性研究奠定基础。 |
ABSTRACT: | OBJECTIVE: To isolate and identify the compositions of alkaloids in Acorus tatarinowii, and to lay the foundation for further study of its pharmacological activity. METHODS: The compositions of alkaloids from A. tatarinowii was separated by cation exchange resin. UPLC-MS method was used to identify the alkaloids. The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1% formic acid-acetonitrile (gradient elution) at a flow rate of 0.3 mL/min. The column temperature was 30 ℃, and the injection volume was 1 μL. ESI ion source was used to detect the fragments, the ion mode scan was positive. Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids. RESULTS: 86 compositions were separated and 5 alkaloids were identified, such as tatarine A, N-trans-feruloyltyramine, N-lauryldiethanolamine, phytosphingosine and 5-butyluridine. CONCLUSIONS: The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A. tatarinowii. |
期刊: | 2019年第30卷第5期 |
作者: | 胡小玲,唐怡,袁金斌 |
AUTHORS: | HU Xiaoling,TANG Yi,YUAN Jinbin |
关键字: | 石菖蒲;生物碱;超高效液相色谱串联质谱法;分离;鉴定 |
KEYWORDS: | Acorus tatarinowii; Alkaloids; UPLC-MS; Isolation; Identification |
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