雷公藤乙酸乙酯部位和水部位化学成分的分离与鉴定
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篇名: 雷公藤乙酸乙酯部位和水部位化学成分的分离与鉴定
TITLE:
摘要: 目的:分离并鉴定雷公藤乙酸乙酯部位和水部位化学成分,为其后续药理研究提供基础。方法:采用MCI GEL-CHP 20P柱层析、C18反相硅胶柱层析、Sephadex LH-20凝胶柱层析、高效液相色谱等方法对雷公藤乙酸乙酯部位和水部位提取物进行分离、纯化,通过核磁共振氢谱、核磁共振碳谱及理化性质对分离的化合物进行结构鉴定。结果:从雷公藤乙酸乙酯部位中分离得到2个化合物,分别鉴定为直楔草酸(化合物1)、邻苯二甲酸二丁酯(化合物2);从雷公藤水部位中分离得到8个糖苷类化合物,分别鉴定为2,6-二甲氧基-4-羟甲基-苯基-1-O-β-D-吡喃葡萄糖苷(化合物3)、2,6-二甲氧基-4-羟基苯酚-1-O-葡萄糖苷(化合物4)、4-羟基-1-(2-羟乙基)-苯基-3-O-β-D-吡喃葡萄糖苷(化合物5)、3,4-二甲氧基-苯基-1-O-β-D-吡喃葡萄糖苷 (化合物6)、β-腺苷(化合物7)、丁香苷(化合物8)、表儿茶素-8-C-β-D-半乳糖苷(化合物9)、2-羟基柚皮素-7-O-β-葡萄糖苷(化合物10)。结论:本研究分离并鉴定了雷公藤乙酸乙酯部位和水部位化学成分。
ABSTRACT: OBJECTIVE: To separate and identify chemical components in ethyl acetate fraction and water fraction from Tripterygium wilfordii, and to provider basis for further pharmacological study. METHODS: The ethyl acetate fraction and water fraction from T. wilfordii were separated and purified by MCI GEL-CHP 20P column chromatography, C18 RP silica gel column chromatography, Sephadex LH-20 gel column chromatography and HPLC. The structures of compounds were analyzed and identified by 1H-NMR, 13C-NMR and physicochemical properties. RESULTS: Two compounds were isolated from ethyl acetate fraction of T. wilfordii, namely orthosphenic acid (compound 1), dibutylphthalate (compound 2). Eight glucosides were isolated from water extract of T. wilfordii, namely 2,6-dimethoxy-4-hydroxymethyl-phenyl-1-O-beta-D-glucopyranoside (compound 3), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucoside(compound 4), 4-hydroxy-1-(2-hydroxyethyl)-phenyl-3-O-β-D-glucopyranoside (compound 5), 3,4-dimethoxy-phenyl-1-O-β-D-glucopyranoside (compound 6),β-adenosine (compound 7), ligustrin (compound 8), epicatechin-8-C-β-D-galactoside (compound 9) and 2-hydroxynaringenin-7-O-β-glucoside (compound 10). CONCLUSIONS: Chemical components of ethyl acetate fraction and water fraction are separated and identified from T. wilfordii.
期刊: 2019年第30卷第5期
作者: 杨瑞昆,邬思芳,闫君,舒积成,张锐,张升林,曹天佑,刘建群
AUTHORS: YANG Ruikun,WU Sifang,YAN Jun,SHU Jicheng,ZHANG Rui,ZHANG Shenglin,CAO Tianyou,LIU Jianqun
关键字: 雷公藤;分离;鉴定;高效液相色谱法;糖苷类化合物
KEYWORDS: Tripterygium wilfordii; Separation; Identification; HPLC; Glucosides compound
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