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伊维菌素对人胃癌细胞BGC-823、MGC-803迁移和侵袭的影响及机制研究
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| 篇名： | 伊维菌素对人胃癌细胞BGC-823、MGC-803迁移和侵袭的影响及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究伊维菌素对人胃癌细胞BGC-823、MGC-803迁移和侵袭能力的影响及其作用机制。方法：0、2.5、5、10、20、40 μmol/L伊维菌素分别作用于BGC-823、MGC-803细胞24 h后用MTT法检测细胞抑制率，再采用Transwell小室侵袭实验观察5 μmol/L伊维菌素和含0.67‰二甲基亚砜的磷酸盐缓冲液（对照组）作用24 h对BGC-823、MGC-803细胞迁移和侵袭的影响，Western blot法分别检测5、10 μmol/L伊维菌素和含0.67‰二甲基亚砜的磷酸盐缓冲液（对照组）作用于BGC-823、MGC-803细胞24 h后上皮-间质转化（EMT）标记物E-cadherin、N-cadherin、Vimentin、Snail和EMT转导通路转化生长因子β（TGF-β）/Smad中TGF-β1、TGF-βR、Smad2、Smad3 蛋白的表达水平。结果：伊维菌素对BGC-823、MGC-803细胞生长均有抑制作用，其细胞抑制率与其浓度呈正相关。与对照组比较，5 μmol/L伊维菌素作用后BGC-823、MGC-803细胞的迁移数和侵袭数均明显减少（P＜0.01或P＜0.001）；5、10 μmol/L伊维菌素作用后BGC-823、MGC-803细胞中E-cadherin蛋白表达明显增强（P＜0.05或P＜0.01或P＜0.001），N-cadherin、Vimentin、Snail、TGF-βR、Smad2、Smad3蛋白表达均明显减弱（P＜0.05或P＜0.01或P＜0.001），TGF-β1蛋白仅在10 μmol/L伊维菌素作用后明显减弱（P＜0.05）。结论：伊维菌素能显著抑制BGC-823、MGC-803细胞的迁移和侵袭，其可能与抑制TGF-β/Smad活性从而影响EMT过程有关。 |
| ABSTRACT： | OBJECTIVE： To study the effects of ivermectin on the migration and invasion of human gastric cancer cell lines BGC-823 and MGC-803 and its mechanism. METHODS： After treated with 0， 2.5， 5， 10， 20， 40 μmol/L ivermectin for 24 h， inhibitory rate of human gastric cancer cell lines BGC-823 and MGC-803 were detected by MTT assay. Effects of 5 μmol/L ivermectin and phosphate buffercontaining 0.67‰ dimethyl sulfoxide （control group） for 24 h on the migration and invasion of` gastric cancer cells BGC-823 and MGC-803 were observed by Transwell chamber invasion assay.Western blot assay was used to detect the protein expression of TGF-β1， TGF-βR， Smad2 and Smad3 in epithelial-mesenchymal transition （EMT） markers E-cadherin， N-cadherin， Vimentin， Snail and EMT transduction pathway TGF-β/smad of BGC-823 and MGC-803 cells after treated with 5， 10 μmol/L ivermectin and phosphate buffercontaining 0.67‰ dimethyl sulfoxide （control group） for 24 h. RESULTS： Ivermectin could inhibit the growth of BGC-823 and MGC-803， inhibitory rate of it was positively correlated with its concentration. Compared with control group， the number of migration and invasion BGC-823 and MGC-803 cells were decreased significantly after treated with 5 μmol/L ivermectin （P＜0.01 or P＜0.001）； the expression of E-cadherin protein was enhanced significantly in BGC-823 and MGC-803 cells after treated with 5 and 10 μmol/L ivermectin （P＜0.05 or P＜0.01 or P＜0.001）； the protein expression of N-cadherin， Vimentin， Snail， TGF-βR， Smad2 and Smad3 were decreased significantly （P＜0.05， P＜0.01 or P＜0.001）； protein expression of TGF-β1 was decreased significantly after treated with 10 μmol/L ivermectin （P＜0.05）. CONCLUSIONS： Ivermectin can significantly inhibit the migration and invasion of gastric cancer cells BGC-823 and MGC-803， and inhibiting the biological activity of EMT by reducing the expression of TGF-β/smad pathway is one of the mechanisms that inhibit the migration and invasion of gastric cancer cells. |
| 期刊： | 2019年第30卷第5期 |
| 作者： | 谢燕娇，邝少轶，邓慧鸣，虞道锐，樊好飞，贾皓，刘嫱 |
| AUTHORS： | XIE Yanjiao，KUANG Shaoyi，DENG Huiming，YU Daorui，FAN Haofei，JIA Hao，LIU Qiang |
| 关键字： | 伊维菌素；人胃癌细胞BGC-823；人胃癌细胞MGC-803；转化生长因子β/Smad；细胞迁移；细胞侵袭 |
| KEYWORDS： | Ivermectin； Human gastric cancer cell BGC- 823； Human gastric cancer cell MGC-803； TGF-β/Smad； Cell migration； Cell invasion |
| 阅读数： | 597 次 |
| 本月下载数： | 3 次 |
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