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生脉饮多糖提取工艺优化及对脾虚模型大鼠肠道功能调节作用的研究
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| 篇名： | 生脉饮多糖提取工艺优化及对脾虚模型大鼠肠道功能调节作用的研究 |
| TITLE： | |
| 摘要： | 目的：优化生脉饮多糖的提取工艺，并探讨生脉饮及其多糖对脾虚模型大鼠肠道功能的调节作用。方法：采用硫酸苯酚法测定多糖含量，并计算多糖提取率；以此为考察指标，采用单因素试验和正交试验，对生脉饮多糖提取工艺的料液比、提取时间、提取温度、提取次数进行优化，并进行验证试验。将80只雄性SD大鼠随机分为空白组，模型组，生脉饮低、中、高剂量组（350、700、1 400 g/L，按生药量计）和生脉饮多糖低、中、高剂量组（24.5、49、98 g/L，按生药量计），每组10只。除空白组外，其余各组大鼠均灌胃大黄水煎液10 mL/kg复制脾虚模型，每天1次，连续15 d。自第16天起，空白组和模型组大鼠均灌胃等容水，其余各组大鼠均灌胃相应药液，每天1次，连续10 d。观察各组大鼠的一般情况，记录体质量，并采用间苯三酚法或酶联免疫吸附测定法检测其血清D-木糖、胃泌素（GAS）、血管活性肠肽（VIP）含量。结果：生脉饮多糖最优提取工艺为料液比1 ∶ 10（g/mL）、提取时间45 min、提取温度80 ℃、提取次数1次。验证试验结果显示，3次提取的多糖提取率分别为7.43％、7.64％、7.80％（RSD＝1.01％，n＝3）。造模后，除空白组外的其余各组大鼠均出现了大便溏稀、体形消瘦、食量减小等症状，且其体质量和血清D-木糖含量均较空白组显著降低（P＜0.01）。末次给药后，各给药组大鼠上述症状均有不同程度的改善，且除模型组外的其余各组大鼠的体质量以及各组大鼠血清D-木糖含量均较同组造模前或给药前显著增加（P＜0.05或P＜0.01）；与空白组比较，模型组大鼠体质量和血清GAS含量均显著降低，血清VIP含量显著增加（P＜0.01）；与模型组比较，生脉饮中剂量组和生脉饮多糖低、高剂量组大鼠体质量以及生脉饮中、高剂量组和生脉饮多糖低、中剂量组大鼠血清D-木糖、GAS含量均显著增加，生脉饮各剂量组和生脉饮多糖低、中剂量组大鼠血清VIP含量均显著降低（P＜0.05或P＜0.01）。结论：优化的生脉饮多糖提取工艺稳定、可行。生脉饮及其多糖可有助于恢复脾虚模型大鼠的肠道功能，这种作用可能与其调节肠道GAS、VIP等因子分泌有关。 |
| ABSTRACT： | OBJECTIVE： To optimize the extraction technology of Shengmaiyin polysaccharide， and to investigate the regulation effects of Shengmaiyin and its polysaccharide on intestinal function of spleen deficiency model rats. METHODS： The contents of polysaccharide were determined by phenol-sulfuric acid method， and the extraction rate of polysaccharide was calculated. Using extraction rate of Shengmaiyin polysaccharide as investigation index， singel factor and orthogonal tests were used to optimize material-liquid ratio， extraction time， extraction temperature and extraction times of Shengmaiyin polysaccharide. Validation test was also conducted. Totally 80 male SD rats were randomly divided into blank group， model group， Shengmaiyin low-dose， medium-dose and high-dose groups （350， 700， 1 400 g/L， by crude drug）， Shengmaiyin polysaccharide low-dose， medium-dose and high-dose groups （24.5， 49， 98 g/L， by crude drug）， with 10 rats in each group. Except for blank group， other groups were given Rheum palmatum water decoction 10 mL/kg to induce spleen deficiency model， once a day， for consecutive 15 d. Since the 16th day， blank group and model group were given isovolumic water intragastrically， while other groups were given corresponding drugs， once a day， for consecutive 10 d. The general status of rats and body weights were recorded in each group. The serum contents of D-xylose， gastrin （GAS） and vasoactive intestinal peptide （VIP） were detected by phloroglucinol method or ELISA. RESULTS： The optimal extraction technology of Shengmaiyin polysaccharide was material-liquid ratio 1 ∶ 10（g/mL）， extraction time 45 min， extraction temperature 80 ℃， extracting for 1 time. Results of validation test showed that extraction rates of the polysaccharide in 3 times were 7.43％， 7.64％， 7.80％ （RSD＝1.01％， n＝3）. After modeling， except for blank group， other groups suffered from loose stools， thin body and reduced food intake， and the body weight and serum level of D-xylose were decreased significantly compared with blank group （P＜0.01）. After last medication， above symptoms of administration groups were improved to different extents. Except for model group， body weight and serum contents of D-xylose in other groups were increased significantly than those before modeling or before medication （P＜0.05 or P＜0.01）. Compared with blank group， body weight and serum content of GAS were decreased significantly in model group， while serum content of VIP was increased significantly （P＜0.01）. Compared with model group， body weight of Shengmaiyin medium-dose group and Shengmaiyin polysaccharide low-dose and high-dose groups， serum contents of D-xylose and GAS in Shengmaiyin medium-dose and high-dose groups and Shengmaiyin polysaccharide low-dose and medium-dose groups were increased significantly， while serum contents of VIP in Shengmaiyin groups and Shengmaiyin polysaccharide low-dose and medium-dose groups were all decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： The optimized extraction technology of Shengmaiyin polysaccharide is stable and feasible. Shengmaiyin and its polysaccharide contribute to the recovery of intestinal function of spleen deficiency model rat， the effects of which may be associated with the secretion regulation of GAS and VIP. |
| 期刊： | 2019年第30卷第4期 |
| 作者： | 游宇，罗林，陈哲杰，林美斯，许何英，熊波，傅超美，谢恬 |
| AUTHORS： | YOU Yu，LUO Lin，CHEN Zhejie，LIN Meisi，XU Heying，XIONG Bo，FU Chaomei，XIE Tian |
| 关键字： | 生脉饮；多糖；多糖提取率；提取工艺；正交试验；脾虚模型；胃泌素；血管活性肠肽；大鼠 |
| KEYWORDS： | Shengmaiyin； Polysaccharide； Extraction rate of polysaccharide； Extraction technology； Orthogonal test； Spleen deficiency model； Gastrin； Vascoactive intestinal peptide； Rats |
| 阅读数： | 262 次 |
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