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理气散结颗粒对肝气郁结模型大鼠的改善作用研究
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| 篇名： | 理气散结颗粒对肝气郁结模型大鼠的改善作用研究 |
| TITLE： | |
| 摘要： | 目的：考察理气散结颗粒对肝气郁结模型大鼠的改善作用。方法：将80只大鼠按体质量随机分为空白对照组（生理盐水）、模型对照组（生理盐水）、逍遥丸对照组（阳性对照a，以生药量计为750 mg/kg）、小金丸对照组（阳性对照b，以丸质量计为200 mg/kg）、理气散结丸对照组（原型对照，以生药量计为1 957 mg/kg）和理气散结颗粒低、中、高剂量组（以生药量计分别为978.5、 1 957、3 914 mg/kg），每组10只。各组大鼠均每天灌胃给药1次，灌胃体积均为20 mL/kg，连续给药21 d；每次给药1 h后，除空白对照组外的其余各组大鼠均采用束缚法复制肝气郁结模型。通过设计糖水偏嗜度实验测定大鼠的糖水偏嗜度、鼠尾悬吊实验测定大鼠悬吊不动时间和挣扎次数、旷场行为学实验测定大鼠行为总得分判断大鼠的肝郁程度以及药物的作用效果。结果：与空白对照组比较，模型对照组大鼠的悬吊不动时间显著延长，糖水偏嗜度和旷场行为学实验中行为总得分显著降低，差异均有统计学意义（P＜0.05或P＜0.01）。与模型对照组比较，小金丸对照组、理气散结丸对照组和理气散结颗粒中剂量组大鼠的挣扎次数显著增加（P＜0.05或P＜0.01）；逍遥丸对照组、小金丸对照组、理气散结丸对照组和理气散结颗粒低、中剂量组大鼠的悬吊不动时间显著缩短，糖水偏嗜度和旷场行为学实验中行为总得分显著升高（P＜0.05或P＜0.01）。与理气散结丸对照组比较，仅理气散结颗粒高剂量组大鼠的挣扎次数显著减少，悬吊不动时间显著延长（P＜0.05或P＜0.01），理气散结颗粒低、中剂量组大鼠上述指标差异均无统计学意义（P＞0.05）。结论：理气散结颗粒对束缚法致肝气郁结模型大鼠有明显的改善作用，且低、中剂量理气散结颗粒与理气散结丸的作用效果相当。 |
| ABSTRACT： | OBJECTIVE： To investigate the improvement effects of Liqi sanjie granule on liver-qi stagnation model rats. METHODS： According to the weight， totally 80 rats were randomly divided into blank control group （normal saline）， model control group （normal saline）， Xiaoyao pill control group （positive control a， 750 mg/kg ，calculated by crude drug）， Xiaojin pill control group （positive control b， 200 mg/kg， calculated by pill weight）， Liqi sanjie pill control group （prototype control， 1 957 mg， calculated by crude drug） and Liqi sanjie granule low-dose， medium-dose and high-dose groups （978.5， 1 957， 3 914 mg/kg， calculated by crude drug）， with 10 rats in each group. Each group was given medicine 20 mL/kg intragastrically once a day， for consecutive 21 d. 1 h after per medication， liver-qi stagnation model was established in those groups by binding method except for blank control group. The syrup preference of rats was determined by designing syrup preference test. Rattail suspension test was adopted to determine the hanging immobility time and struggling times of mice. Open-field behavior test was used to determine total behavior score so as to judge the extent of liver-qi stagnation and effect of the drug in rats. RESULTS： Compared with blank control group， hanging immobility time of model control group was significantly prolonged， the syrup preference and the total behavior score of open field test were decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. Compared with model control group， the struggling times of rats were increased significantly in Xiaojin pill control group， Liqi sanjie pill control group and Liqi sanjie granule medium-dose group （P＜0.05 or P＜0.01）； the hanging immobility time of Xiaoyao pill control group， Xiaojin pill control group， Liqi sanjie pill control group， Liqi sanjie granule low-dose and medium-dose groups were shortened significantly； syrup preference and total behavior score of open-field behavior test were increased significantly （P＜0.05 or P＜0.01）. Compared with Liqi sanjie pill control group， the struggling times of rats were decreased significantly and hanging immobility time were prolonged significantly only in Liqi sanjie granule high-dose group （P＜0.05 or P＜0.01）； there was no statistical significance in above indexes of rats in Liqi sanjie granule low-dose and medium-dose groups （P＞0.05）. CONCLUSIONS： Liqi sanjie granule can significantly improve liver-qi stagnation caused by binding method， and the effects of low-dose and medium-dose Liqi sanjie granule are similar to those of Liqi sanjie pill. |
| 期刊： | 2019年第30卷第3期 |
| 作者： | 肖成，胡凤娇，魏谭军，周殿儒，梁源，王张，孙位军，王毅 |
| AUTHORS： | XIAO Cheng，HU Fengjiao，WEI Tanjun，ZHOU Dianru，LIANG Yuan，WANG Zhang，SUN Weijun，WANG Yi |
| 关键字： | 理气散结颗粒；肝气郁结模型；束缚法；行为学；抑郁；大鼠 |
| KEYWORDS： | Liqi sanjie granule； Liver-qi stagnation model； Binding method； Open-field behavior； Depression； Rat |
| 阅读数： | 285 次 |
| 本月下载数： | 2 次 |
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