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松果菊苷对低压低氧环境下小鼠空间认知功能的改善作用及机制研究
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| 篇名： | 松果菊苷对低压低氧环境下小鼠空间认知功能的改善作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究松果菊苷（ECH）对低压低氧环境下小鼠空间认知功能的改善作用及其作用机制。方法：将60只小鼠随机分为空白组（生理盐水）、模型组（生理盐水）、阳性组（银杏叶提取物片，100 mg/kg）和ECH低、中、高剂量组（50、75、100 mg/kg），每组10只。除空白组外，其余各组小鼠均饲养于低压氧仓模拟低压低氧，各组小鼠每天灌胃给药1次，连续给药7 d（给药后立即放入低压氧仓内）；以2 min内小鼠水平与垂直活动次数为指标，评价其负面情绪与空间认知功能；苏木精-伊红染色后显微镜观察小鼠海马组织病理学变化；并检测小鼠海马组织中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）的水平。结果：与空白组比较，模型组小鼠水平活动次数、MDA水平均显著增加/升高（P＜0.05）；垂直活动次数，SOD、CAT、GSH-Px水平均显著减少/降低（P＜0.05）；海马组织CA1区锥体细胞排列疏松，多数锥体细胞紧缩深染。与模型组比较，阳性组和ECH高剂量组小鼠水平活动次数、MDA水平显著减少/降低（P＜0.05）；垂直活动次数，SOD、CAT、GSH-Px水平显著增加/升高（P＜0.05）；海马组织CA1区锥体细胞数量丰富，排列紧密，少量锥体细胞紧缩深染。结论：ECH对低压低氧环境下小鼠空间认知功能障碍具有一定的改善作用，其机制可能与升高海马组织中SOD、CAT、GSH-Px水平及降低MDA水平有关。 |
| ABSTRACT： | OBJECTIVE： To study the improving effects of echinacoside （ECH） on spatial cognitive function in mice under hypobaric hypoxia environment and its mechanism. METHODS： Totally 60 mice were randomly divided into blank group （normal saline）， model group （normal saline）， positive group （Ginkgo leaf extract tablet，100 mg/kg） and ECH low-dose， medium-dose and high-dose groups （50， 75， 100 mg/kg）， with 10 mice in each group. Except for blank group， other groups were cultured in hypobaric oxygen chamber to simulate hypobaric hypoxia； they were given relevant medicine intragastrically once a day， for consecutive 7 d （Placing into hypobaric oxygen chamber immediately after medication）. Using the times of horizontal and vertical activities of mice in 2 min as index， negative emotions and spatial cognitive function were evaluated. Histopathological changes of hippocampus in mice were observed by microscopy after HE staining. The levels of SOD， CAT， GSH-Px and MDA in hippocampal tissue of mice were detected. RESULTS： Compared with blank group， the times of horizontal activities， MDA level were increased significantly in model group （P＜0.05）， while the times of vertical activities， the levels of SOD， CAT and GSH-Px were decreased significantly （P＜0.05）； the pyramidal cells in the CA1 area of the hippocampal tissue were arranged loosely， and many pyramidal cells were compressed and stained deeply. Compared with model group， the times of horizontal activities and MDA level were decreased significantly in positive group and ECH high-dose group （P＜0.05）， while the times of vertical activities， the levels of SOD， CAT and GSH-Px were increased significantly （P＜0.05）； the pyramidal cells in the CA1 region of the hippocampal tissue were abundant and closely arranged， and a few of them are constricted and deeply stained. CONCLUSIONS： ECH can improve spatial cognitive impairment of mice under hypobaric hypoxia environment， the mechanism of which may be associated with up-regulation of SOD， CAT and GSH-Px， down-regulation of MDA in the hippocampal tissue. |
| 期刊： | 2019年第30卷第3期 |
| 作者： | 郑洪南，苏钰婷，赫雪锋，王剑波 |
| AUTHORS： | ZHENG Hongnan，SU Yuting，HE Xuefeng，WANG Jianbo |
| 关键字： | 松果菊苷；低压低氧；空间认知功能；海马组织；小鼠 |
| KEYWORDS： | Echinacoside； Hypobaric hypoxia； Spatial cognitive function； Hippocampal tissue； Mice |
| 阅读数： | 448 次 |
| 本月下载数： | 5 次 |
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