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甘草水提物对雷公藤甲素致大鼠急性肝损伤的改善作用及对其体内IL-10、TNF-α水平的影响
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| 篇名： | 甘草水提物对雷公藤甲素致大鼠急性肝损伤的改善作用及对其体内IL-10、TNF-α水平的影响 |
| TITLE： | |
| 摘要： | 目的：观察甘草水提物对雷公藤甲素致大鼠急性肝损伤的改善作用及对其体内IL-10、TNF-α水平的影响。方法：60只健康雄性SD大鼠随机分为空白对照组、模型组、阳性对照组（异甘草酸镁）和甘草水提物低、中、高剂量组。空白对照组、模型组大鼠灌胃等容生理盐水，阳性对照组大鼠灌胃异甘草酸镁药液（13.5 mg/kg），甘草水提物低、中、高剂量组大鼠灌胃甘草水提物药液（120、240、480 mg/kg，以提取物量计），给药体积均为1 mL，每天1次，连续给药7 d。第8 天时，模型组、阳性对照组和甘草水提物各剂量组大鼠均一次性灌胃雷公藤甲素药液（0.6 mg/kg）以建立急性肝损伤模型。采用苏木精-伊红染色法检查大鼠肝组织病理学变化；采用酶偶联反应法检测各组大鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）的活性；采用Western blotting法检测大鼠肝组织中白细胞介素10（IL-10）、肿瘤坏死因子α（TNF-α）的蛋白表达水平。结果：与空白对照组比较，模型组大鼠肝组织出现明显病理学变化，血清中ALT、AST活性均显著升高，肝组织中TNF-α蛋白表达水平显著升高、IL-10蛋白表达水平显著降低（P＜0.01）。与模型组比较，甘草水提物各剂量组大鼠肝组织病理学变化程度明显减轻；除甘草水提物低剂量组大鼠血清中AST活性外，甘草水提物各剂量组大鼠血清中ALT、AST活性和肝组织中TNF-α蛋白表达水平均显著降低，肝组织中IL-10蛋白表达水平均显著升高（P＜0.05或P＜0.01）。结论：甘草水提物能减轻雷公藤甲素所致大鼠急性肝损伤，其机制可能与下调促炎因子TNF-α的表达、上调抗炎因子IL-10的表达有关。 |
| ABSTRACT： | OBJECTIVE： To observe the improvement effects of water extract of Glycyrrhiza uralensis on acute hepatic injury caused by triptolide and its effects on the levels of IL-10 and TNF-α in rats. METHODS： A total of 60 healthy male SD rats were randomized into blank control group， model group， positive control group （magnesium isoglycyrrhizinate） and G. uralensis water extract low-dose， middle-dose and high-dose groups. Blank control group and model group were given isochoric normal saline intragastrically； positive control group was given Magnesium isoglycyrrhizinate liquid intragastrically （13.5 mg/kg）； G. uralensis water extract low-dose， middle-dose and high-dose groups were given G. uralensis water extract intragastrically 1 mL （120， 240， 480 mg/kg， by the amount of extract）， qd， for consecutive 7 d. At 8th day of administration， model group， positive control group and G. uralensis water extract groups were given Triptolide liquid intragastrically （0.6 mg/kg） to establish acute liver injury model. HE staining was used to detect the pathological changes of liver tissue in rats. The activities of ALT and AST in serum were determined by enzyme coupling reaction. The protein expression of IL-10 and TNF-α in liver tissue were analyzed by Western blotting. RESULTS： Compared with blank control group， obvious pathological changes of liver tissue in rats were observed in model group； serum activities of ALT and AST， the protein expression level of TNF-α in liver tissue were increased significantly， while the protein expression level of IL-10 was decreased significantly （P＜0.01）. Compared with model group， pathological changes of liver tissue were relieved significantly. Except for the activity of AST in serum of rats in G. uralensis water extract low-dose group， the activities of ALT and AST in serum， the protein expression level of TNF-α in liver tissue were decreased significantly in G. uralensis groups， while the protein expression level of IL-10 in liver tissue was increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： G. uralensis water extract can relieve triptolide-induced acute liver injury in rats， the mechanism of which may be associated with expression down-regulation of pro-inflammatory factor TNF-α and expression up-regulation of anti-inflammatory factor IL-10. |
| 期刊： | 2019年第30卷第2期 |
| 作者： | 朱胜楠，张靖，谭亲友，胡骞，苏华珍，黄少远，刘芳 |
| AUTHORS： | ZHU Shengnan，ZHANG Jing，TAN Qinyou，HU Qian，SU Huazhen，HUANG Shaoyuan，LIU Fang |
| 关键字： | 甘草水提物；雷公藤甲素；大鼠；急性肝损伤；白细胞介素10；肿瘤坏死因子α |
| KEYWORDS： | Glycyrrhiza uralensis water extract； Triptolide； Rats； Acute liver injury； IL-10； TNF-α |
| 阅读数： | 246 次 |
| 本月下载数： | 5 次 |
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