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紫荆叶提取物对酪氨酸酶活性抑制作用的谱效关系研究
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| 篇名： | 紫荆叶提取物对酪氨酸酶活性抑制作用的谱效关系研究 |
| TITLE： | |
| 摘要： | 目的：研究紫荆叶提取物对酪氨酸酶活性抑制作用的谱效关系。方法：取不同采收时期的紫荆叶样品16批，以70％乙醇进行加热提取。采用高效液相色谱法对紫荆叶提取物进行分析，色谱柱为InertSustain C18，流动相为甲醇-0.1％冰醋酸溶液（梯度洗脱），流速为0.8 mL/min，柱温为30 ℃，检测波长为254 nm，进样量为10 μL。将所获得的16个批次紫荆叶提取物色谱图采用《中药色谱指纹图谱相似度评价系统2004（A版）》进行匹配，获得量化特征色谱图并确定共有峰。采用体外酪氨酸酶多巴速率氧化微量法测定紫荆叶提取物对酪氨酸酶活性的抑制率。采用偏最小二乘回归分析法，以紫荆叶提取物特征共有峰峰面积为自变量、其对酪氨酸酶活性的抑制率为因变量进行谱效关系分析，筛选出与酪氨酸酶活性抑制作用有显著相关性的色谱峰。结果与结论：匹配得到紫荆叶提取物的16个特征共有峰；酪氨酸酶活性抑制试验结果显示，紫荆叶提取物质量浓度为100 mg/mL（按生药计）时对酪氨酸酶活性的抑制作用最强；谱效关系分析结果显示，紫荆叶提取物特征色谱图中P5、P8、P10、P12、P15、P16号共有峰与其对酪氨酸酶活性的抑制作用呈正相关（相关系数R＞0.1），P2、P4号共有峰与其对酪氨酸酶活性的抑制作用呈负相关（相关系数绝对值│R│＞0.1），提示上述特征共有峰代表的化合物可能对酪氨酸酶活性具有抑制或激活作用。 |
| ABSTRACT： | OBJECTIVE： To study the spectrum-effect relationship of inhibitory effects of the extract of Cercis chinensis leaves on the activity of tyrosinase. METHODS： Totally 16 batches of samples of C. chinensis leaves were collected at different harvesting periods， and were heated and extracted with 70％ ethanol. The extract of C. chinensis leaves was analyzed by HPLC. The determination was performed on InertSustain C18 column with mobile phase consisted of methanol-0.1％ glacial acetic acid （gradient elution） at the flow rate of 0.8 mL/min. The column temperature was 30 ℃， and the detection wavelength was set at 254 nm. The sample size was 10 μL. The chromatograms of 16 batches of extract of C. chinensis leaves were matched with Similarity Evaluation System for Fingerprint Chromatogram of TCM 2004 （A edition） to obtain characteristic chromatogram and define common peak. The inhibitory rate of the extract of C. chinensis leaves on the activity of tyrosinase was detected by in vitro tyrosinase dopamine rate oxidation microassay. Partial least squares regression analysis was used to analyze the spectrum-effect relationship of the extract of C. chinensis leaves using common peak area as independent variable， inhibitory rate to tyrosinase as dependent variable. The chromatographic peaks with significant correlation with tyrosinase inhibition effects were screened out. RESULTS & CONCLUSIONS： Totally 16 characteristic common peaks were obtained for the extract of C. chinensis leaves. Tyrosinase activity test showed that the inhibition effect of the extract of C. chinensis leaves was the strongest to tyrosinase when the concentration of the extract was 100 mg/mL （by crude drug）. The common peaks P5， P8， P10， P12， P15， P16 had positive correlation with tyrosinase inhibition effects （correlation coefficient R＞0.1）， while the common peaks P2 and P4 had negative correlation with tyrosinase inhibition effects （absolute correlation coefficient │R│＞0.1）， indicating compounds represented by these common peaks may inhibit or activate tyrosinase activity. |
| 期刊： | 2018年第29卷第24期 |
| 作者： | 孙慧玲，何楠，史梦珺，Adel Fahmi Ahmed，李昌勤 |
| AUTHORS： | SUN Huiling，HE Nan，SHI Mengjun，Adel Fahmi Ahmed，LI Changqin |
| 关键字： | 紫荆叶；提取物；活性成分；酪氨酸酶；抑制作用；谱效关系 |
| KEYWORDS： | Cercis chinensis leaves； Extract； Active component； Tyrosinase； Inhibition effect； Spectrum-effect relationship |
| 阅读数： | 403 次 |
| 本月下载数： | 10 次 |
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