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祛湿化瘀方对非酒精性脂肪肝模型大鼠血清游离脂肪酸谱的影响
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| 篇名： | 祛湿化瘀方对非酒精性脂肪肝模型大鼠血清游离脂肪酸谱的影响 |
| TITLE： | |
| 摘要： | 目的：探讨祛湿化瘀方对高脂饮食诱导的非酒精性脂肪肝（NAFLD）模型大鼠血清游离脂肪酸谱的影响。方法：30只雄性SD大鼠按计算机随机数字表分为正常组、模型组、祛湿化瘀方组，每组10只。模型组和祛湿化瘀方组大鼠饲喂高脂饲料建立NAFLD模型，正常组大鼠饲喂普通饲料，持续8周；自第5周起，祛湿化瘀方组大鼠按0.1 mL/kg灌胃祛湿化瘀方药液，正常组和模型组大鼠灌胃等量生理盐水，连续给药4周。8周后处死大鼠，取肝组织样本，采用苏木精-伊红染色法观察肝组织病理学变化；取血清样本采用气相色谱-质谱联用技术，以水杨酸甲酯为内标进样测定，按标准曲线法计算血清中游离脂肪酸的含量。结果：与正常组比较，模型组大鼠肝组织多发坏死肝细胞，肝细胞出现脂肪样变性和空泡变性；血清中月桂酸、肉豆蔻酸、十五烷酸、棕榈酸、十七烷酸、硬脂酸、花生酸、二十一烷酸、榆树酸等饱和脂肪酸含量均显著升高（P＜0.05）；顺-10-十七碳烯酸、油酸、α-亚麻酸、γ-亚麻酸、芥酸、亚油酸、顺-11-二十碳烯酸、花生四烯酸、顺-11，14，17-二十碳三烯酸、顺-4，7，10，13，16，19-二十二碳六烯酸（DHA）等不饱和脂肪酸含量均显著降低（P＜0.05或P＜0.01）。与模型组比较，祛湿化瘀方组大鼠肝组织病理学变化程度明显减轻；血清中上述饱和脂肪酸含量均显著降低，不饱和脂肪酸含量均显著升高（P＜0.05）。结论：祛湿化瘀方干预能显著改善高脂饮食诱导的NAFLD模型大鼠的肝组织病理学变化，且能显著改善其血清中饱和脂肪酸和不饱和脂肪酸含量的异常变化，调节紊乱的游离脂肪酸谱趋于正常。建议通过膳食补充ω-3多不饱和脂肪酸或采用祛湿化瘀方等药物干预脂肪酸代谢，从而有效预防和治疗NAFLD。 |
| ABSTRACT： | OBJECTIVE： To investigate the effects of Qushi huayu formula on serum free fatty acid profile in non-alcoholic fatty liver disease （NAFLD） model rats. METHODS： A total of 30 SD male rats were divided into normal group， model group， Qushi huayu formula group， with 10 rats in each group according to random number table. Model group and Qushi huayu formula group were given high-lipid diet to establish NAFLD model； normal group was given common diet for 8 weeks. Since 5th week， Qushi huayu formula group was given Qushi huayu formula liquid 0.1 mL/kg intragastrically； normal group and model group were given constant volume of normal saline intragastrically for consecutive 4 weeks. 8 weeks later， the rats were sacrificed and liver tissue was collected. Histopathological changes of liver were observed by HE staining. Serum samples were collected and determined by GC/MS using menthyl salicylate as internal standard. The content of free fatty acid in serum was calculated by standard curve method. RESULTS： Compared with normal group， hepatocytes of rats in model group were necrotic， and fatty degeneration and vacuolar degeneration of hepatocytes appeared. The serum contents of saturated fatty acids as lauric acid， myristic acid， pentadecanoic acid， palmitic acid， heptadecanoic acid， stearic acid， arachidic acid， heneicosanic acid and docosanoic acid were increased significantly （P＜0.05）； the contents of unsaturated fatty acids as cis-10-heptadecenoic acid， oleic acid， α-linolenic acid， γ-linolenic acid， erucic acid， linoleic acid， cis-11-eicosenoic acid， arachidonic acid， cis-11， 14， 17-eicosatrienoic acid， cis-4， 7， 10， 13，16，19-docosahexenoic acid （DHA） were decreased significantly （P＜0.05 or P＜0.01）. Compared with model group， histopathological changes of liver was relieved significantly in Qushi huayu formula group； serum contents of above saturated fatty acids were decreased significantly， while the contents of above unsaturated fatty acids were increased significantly （P＜0.05）. CONCLUSIONS： Qushi huayu formula can significantly improve histopathological changes of liver in high-lipid diet-induced NAFLD model rats， and can significantly improve the abnormal changes in the serum contents of saturated fatty acids and unsaturated fatty acids so that free fatty acid profile return to normal. It is suggested to supplement ω-3 polyunsaturated fatty acids or using medicine like Qushi huayu formula to intervene in fatty acid metabolism so as to effectively prevent and treat NAFLD. |
| 期刊： | 2018年第29卷第24期 |
| 作者： | 苟小军，冯琴，胡义扬 |
| AUTHORS： | GOU Xiaojun， FENG Qin， HU Yiyang |
| 关键字： | 祛湿化瘀方；非酒精性脂肪肝；大鼠；脂肪酸谱；游离脂肪酸 |
| KEYWORDS： | Qushi huayu formula； Non-alcoholic fatty liver disease； Rat； Fatty acid profile； Free fatty acid |
| 阅读数： | 345 次 |
| 本月下载数： | 4 次 |
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